Direct detection of double-stranded DNA: Molecular methods and applications for DNA diagnostics

Indraneel Ghosh, Cliff I. Stains, Aik T. Ooi, David Segal

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Methodologies to detect DNA sequences with high sensitivity and specificity have tremendous potential as molecular diagnostic agents. Most current methods exploit the ability of single-stranded DNA (ssDNA) to base pair with high specificity to a complementary molecule. However, recent advances in robust techniques for recognition of DNA in the major and minor groove have made possible the direct detection of double-stranded DNA (dsDNA), without the need for denaturation, renaturation, or hybridization. This review will describe the progress in adapting polyamides, triplex DNA, and engineered zinc finger DNA-binding proteins as dsDNA diagnostic systems. In particular, the sequence-enabled reassembly (SEER) method, involving the use of custom zinc finger proteins, offers the potential for direct detection of dsDNA in cells, with implications for cell-based diagnostics and therapeutics.

Original languageEnglish (US)
Pages (from-to)551-560
Number of pages10
JournalMolecular BioSystems
Volume2
Issue number11
DOIs
StatePublished - 2006

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Zinc Fingers
Zinc
Denaturation
Molecular Pathology
DNA
Nylons
Base Pairing
Carrier Proteins
Sensitivity and Specificity
Molecules
Proteins
Therapeutics

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

Cite this

Direct detection of double-stranded DNA : Molecular methods and applications for DNA diagnostics. / Ghosh, Indraneel; Stains, Cliff I.; Ooi, Aik T.; Segal, David.

In: Molecular BioSystems, Vol. 2, No. 11, 2006, p. 551-560.

Research output: Contribution to journalArticle

Ghosh, Indraneel ; Stains, Cliff I. ; Ooi, Aik T. ; Segal, David. / Direct detection of double-stranded DNA : Molecular methods and applications for DNA diagnostics. In: Molecular BioSystems. 2006 ; Vol. 2, No. 11. pp. 551-560.
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