Direct detection of aptamer-thrombin binding via surface-enhanced Raman spectroscopy

Cynthia V. Pagba, Stephen M. Lane, Hansang Cho, Sebastian Wachsmann-Hogiu

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

In this study, we exploit the sensitivity offered by surfaceenhanced Raman scattering (SERS) for the direct detection of thrombin using the thrombin-binding aptamer (TBA) as molecular receptor. The technique utilizes immobilized silver nanoparticles that are functionalized with thiolated thrombin-specific binding aptamer, a 15-mer (5'-GGTTGGTGTGGTTGG-3') quadruplex forming oligonucleotide. In addition to the Raman vibrational bands corresponding to the aptamer and blocking agent, new peaks (mainly at 1140, 1540, and 1635 cm-1) that are characteristic of the protein are observed upon binding of thrombin. These spectral changes are not observed when the aptamer-nanoparticle assembly is exposed to a nonbinding protein such as bovine serum albumin (BSA). This methodology could be further used for the development of label-free biosensors for direct detection of proteins and other molecules of interest for which aptamers are available.

Original languageEnglish (US)
Article number047006
JournalJournal of Biomedical Optics
Volume15
Issue number4
DOIs
StatePublished - Jul 2010

Fingerprint

thrombin
Thrombin
Raman spectroscopy
Proteins
proteins
Nanoparticles
Oligonucleotides
Bovine Serum Albumin
Silver
Biosensors
nanoparticles
Raman scattering
oligonucleotides
Labels
albumins
bioinstrumentation
serums
Molecules
assembly
silver

Keywords

  • Aptamer
  • Direct detection
  • Label-free assay
  • Quadruplex
  • Surface-enhanced raman scattering
  • Thrombin

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

Direct detection of aptamer-thrombin binding via surface-enhanced Raman spectroscopy. / Pagba, Cynthia V.; Lane, Stephen M.; Cho, Hansang; Wachsmann-Hogiu, Sebastian.

In: Journal of Biomedical Optics, Vol. 15, No. 4, 047006, 07.2010.

Research output: Contribution to journalArticle

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