Abstract
In this study, we exploit the sensitivity offered by surfaceenhanced Raman scattering (SERS) for the direct detection of thrombin using the thrombin-binding aptamer (TBA) as molecular receptor. The technique utilizes immobilized silver nanoparticles that are functionalized with thiolated thrombin-specific binding aptamer, a 15-mer (5'-GGTTGGTGTGGTTGG-3') quadruplex forming oligonucleotide. In addition to the Raman vibrational bands corresponding to the aptamer and blocking agent, new peaks (mainly at 1140, 1540, and 1635 cm-1) that are characteristic of the protein are observed upon binding of thrombin. These spectral changes are not observed when the aptamer-nanoparticle assembly is exposed to a nonbinding protein such as bovine serum albumin (BSA). This methodology could be further used for the development of label-free biosensors for direct detection of proteins and other molecules of interest for which aptamers are available.
| Original language | English (US) |
|---|---|
| Article number | 047006 |
| Journal | Journal of Biomedical Optics |
| Volume | 15 |
| Issue number | 4 |
| DOIs | |
| State | Published - Jul 2010 |
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Keywords
- Aptamer
- Direct detection
- Label-free assay
- Quadruplex
- Surface-enhanced raman scattering
- Thrombin
ASJC Scopus subject areas
- Biomedical Engineering
- Biomaterials
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
Cite this
Direct detection of aptamer-thrombin binding via surface-enhanced Raman spectroscopy. / Pagba, Cynthia V.; Lane, Stephen M.; Cho, Hansang; Wachsmann-Hogiu, Sebastian.
In: Journal of Biomedical Optics, Vol. 15, No. 4, 047006, 07.2010.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Direct detection of aptamer-thrombin binding via surface-enhanced Raman spectroscopy
AU - Pagba, Cynthia V.
AU - Lane, Stephen M.
AU - Cho, Hansang
AU - Wachsmann-Hogiu, Sebastian
PY - 2010/7
Y1 - 2010/7
N2 - In this study, we exploit the sensitivity offered by surfaceenhanced Raman scattering (SERS) for the direct detection of thrombin using the thrombin-binding aptamer (TBA) as molecular receptor. The technique utilizes immobilized silver nanoparticles that are functionalized with thiolated thrombin-specific binding aptamer, a 15-mer (5'-GGTTGGTGTGGTTGG-3') quadruplex forming oligonucleotide. In addition to the Raman vibrational bands corresponding to the aptamer and blocking agent, new peaks (mainly at 1140, 1540, and 1635 cm-1) that are characteristic of the protein are observed upon binding of thrombin. These spectral changes are not observed when the aptamer-nanoparticle assembly is exposed to a nonbinding protein such as bovine serum albumin (BSA). This methodology could be further used for the development of label-free biosensors for direct detection of proteins and other molecules of interest for which aptamers are available.
AB - In this study, we exploit the sensitivity offered by surfaceenhanced Raman scattering (SERS) for the direct detection of thrombin using the thrombin-binding aptamer (TBA) as molecular receptor. The technique utilizes immobilized silver nanoparticles that are functionalized with thiolated thrombin-specific binding aptamer, a 15-mer (5'-GGTTGGTGTGGTTGG-3') quadruplex forming oligonucleotide. In addition to the Raman vibrational bands corresponding to the aptamer and blocking agent, new peaks (mainly at 1140, 1540, and 1635 cm-1) that are characteristic of the protein are observed upon binding of thrombin. These spectral changes are not observed when the aptamer-nanoparticle assembly is exposed to a nonbinding protein such as bovine serum albumin (BSA). This methodology could be further used for the development of label-free biosensors for direct detection of proteins and other molecules of interest for which aptamers are available.
KW - Aptamer
KW - Direct detection
KW - Label-free assay
KW - Quadruplex
KW - Surface-enhanced raman scattering
KW - Thrombin
UR - http://www.scopus.com/inward/record.url?scp=79952201065&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79952201065&partnerID=8YFLogxK
U2 - 10.1117/1.3465594
DO - 10.1117/1.3465594
M3 - Article
VL - 15
JO - Journal of Biomedical Optics
JF - Journal of Biomedical Optics
SN - 1083-3668
IS - 4
M1 - 047006
ER -