Abstract
A partially purified Bacillus thuringiensis var. israelensis (Bti) delta -endotoxin was used to immunize rabbits. The antisera obtained have an improved specificity towards the mosquito larvacidal activity of the toxin, as opposed to antiserum raised when the whole crystal was used as immunogen. Using a two step/indirect ELISA (enzyme linked immunosorbent assay) procedure, fourteen experimental formulations were tested, and the results were compared with bioassays. An average of 69. 1 international units plus or minus 20% c. v. was found to associate with each ug of toxin detected by the ELISA.
| Original language | English (US) |
|---|---|
| Title of host publication | ACS Symposium Series |
| Place of Publication | Washington, DC, USA |
| Publisher | ACS |
| Pages | 317 |
| Number of pages | 1 |
| ISBN (Print) | 0841209103 |
| State | Published - 1985 |
Fingerprint
ASJC Scopus subject areas
- Chemistry(all)
Cite this
DIRECT CORRELATION BETWEEN BIOASSAY AND ELISA FOR THE BACILLUS THURINGIENSIS VAR. ISRAELENSIS delta -ENDOTOXIN. / Cheung, Peter Y K; Hammock, Bruce D.
ACS Symposium Series. Washington, DC, USA : ACS, 1985. p. 317.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - DIRECT CORRELATION BETWEEN BIOASSAY AND ELISA FOR THE BACILLUS THURINGIENSIS VAR. ISRAELENSIS delta -ENDOTOXIN.
AU - Cheung, Peter Y K
AU - Hammock, Bruce D.
PY - 1985
Y1 - 1985
N2 - A partially purified Bacillus thuringiensis var. israelensis (Bti) delta -endotoxin was used to immunize rabbits. The antisera obtained have an improved specificity towards the mosquito larvacidal activity of the toxin, as opposed to antiserum raised when the whole crystal was used as immunogen. Using a two step/indirect ELISA (enzyme linked immunosorbent assay) procedure, fourteen experimental formulations were tested, and the results were compared with bioassays. An average of 69. 1 international units plus or minus 20% c. v. was found to associate with each ug of toxin detected by the ELISA.
AB - A partially purified Bacillus thuringiensis var. israelensis (Bti) delta -endotoxin was used to immunize rabbits. The antisera obtained have an improved specificity towards the mosquito larvacidal activity of the toxin, as opposed to antiserum raised when the whole crystal was used as immunogen. Using a two step/indirect ELISA (enzyme linked immunosorbent assay) procedure, fourteen experimental formulations were tested, and the results were compared with bioassays. An average of 69. 1 international units plus or minus 20% c. v. was found to associate with each ug of toxin detected by the ELISA.
UR - http://www.scopus.com/inward/record.url?scp=0021857331&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021857331&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:0021857331
SN - 0841209103
SP - 317
BT - ACS Symposium Series
PB - ACS
CY - Washington, DC, USA
ER -