DIRECT CORRELATION BETWEEN BIOASSAY AND ELISA FOR THE BACILLUS THURINGIENSIS VAR. ISRAELENSIS delta -ENDOTOXIN.

Peter Y K Cheung, Bruce D. Hammock

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

A partially purified Bacillus thuringiensis var. israelensis (Bti) delta -endotoxin was used to immunize rabbits. The antisera obtained have an improved specificity towards the mosquito larvacidal activity of the toxin, as opposed to antiserum raised when the whole crystal was used as immunogen. Using a two step/indirect ELISA (enzyme linked immunosorbent assay) procedure, fourteen experimental formulations were tested, and the results were compared with bioassays. An average of 69. 1 international units plus or minus 20% c. v. was found to associate with each ug of toxin detected by the ELISA.

Original languageEnglish (US)
Title of host publicationACS Symposium Series
Place of PublicationWashington, DC, USA
PublisherACS
Pages317
Number of pages1
ISBN (Print)0841209103
StatePublished - 1985

ASJC Scopus subject areas

  • Chemistry(all)

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