Abstract
A partially purified Bacillus thuringiensis var. israelensis (Bti) delta -endotoxin was used to immunize rabbits. The antisera obtained have an improved specificity towards the mosquito larvacidal activity of the toxin, as opposed to antiserum raised when the whole crystal was used as immunogen. Using a two step/indirect ELISA (enzyme linked immunosorbent assay) procedure, fourteen experimental formulations were tested, and the results were compared with bioassays. An average of 69. 1 international units plus or minus 20% c. v. was found to associate with each ug of toxin detected by the ELISA.
| Original language | English (US) |
|---|---|
| Title of host publication | ACS Symposium Series |
| Place of Publication | Washington, DC, USA |
| Publisher | ACS |
| Pages | 317 |
| Number of pages | 1 |
| ISBN (Print) | 0841209103 |
| State | Published - 1985 |
ASJC Scopus subject areas
- Chemistry(all)
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Cheung, P. Y. K., & Hammock, B. D. (1985). DIRECT CORRELATION BETWEEN BIOASSAY AND ELISA FOR THE BACILLUS THURINGIENSIS VAR. ISRAELENSIS delta -ENDOTOXIN. In ACS Symposium Series (pp. 317). ACS.