Abstract
Background: There is evidence that endothelin-1 (ET-1) increases extravasation of fluid and protein into vascular beds. The present study was designed to determine the direct effects of ET-1 on hydraulic permeability (L(p)) when microvascular hydraulic and oncotic pressures are controlled. Methods: Postcapillary venules in the rat mesentery were perfused in situ and paired measurements of L(p) obtained by using the modified Landis micro-occlusion method. L(p) measured after a 15-minute perfusion with Ringer's albumin solution (control) was compared with L(p) after a subsequent 15-minute perfusion with one of three treatments: control (n = 4), 8 pM ET-1 (n = 6), or 80 pM ET-1 (n = 6). Results: Baseline L(p) for all vessels averaged (± SE) 8.1 ± 0.8 x 10-8 cm · sec-1 · cm H2O-1 and was not significantly different between groups. Perfusion with either control or 8 pM ET-1 did not significantly change the L(p) of any of the vessels. Significant decreases in L(p) of 40 to 60% were observed in venules perfused with 80 pM ET-1. The average L(p) in this group was 9.9 ± 1.4 during baseline and decreased to 5.0 ± 0.7 during ET-1 perfusion (p = 0.003). Washout of 80 pM ET-1 for periods of up to 15 minutes did not return L(p) to baseline values. Conclusion: Low-dose ET-1 did not directly increase Lp in postcapillary venules. ET-1 at 80 pM, however, significantly decreased L(p). These data implicate factors other than a direct permeability-increasing effect in ET-1. At higher concentrations, ET-1 may have a protective effect on endothelial barrier function.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 713-718 |
| Number of pages | 6 |
| Journal | Journal of Trauma - Injury, Infection and Critical Care |
| Volume | 47 |
| Issue number | 4 |
| DOIs | |
| State | Published - Oct 1999 |
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Keywords
- Fluid filtration
- Landis micro-occlusion technique
- Mesentery
- Microvascular permeability
- Postcapillary venules
ASJC Scopus subject areas
- Surgery
Cite this
Direct actions of endothelin-1 on single vessel hydraulic permeability. / Victorino, Gregory P.; Wisner, David H; Tucker, Vicky L.
In: Journal of Trauma - Injury, Infection and Critical Care, Vol. 47, No. 4, 10.1999, p. 713-718.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Direct actions of endothelin-1 on single vessel hydraulic permeability
AU - Victorino, Gregory P.
AU - Wisner, David H
AU - Tucker, Vicky L.
PY - 1999/10
Y1 - 1999/10
N2 - Background: There is evidence that endothelin-1 (ET-1) increases extravasation of fluid and protein into vascular beds. The present study was designed to determine the direct effects of ET-1 on hydraulic permeability (L(p)) when microvascular hydraulic and oncotic pressures are controlled. Methods: Postcapillary venules in the rat mesentery were perfused in situ and paired measurements of L(p) obtained by using the modified Landis micro-occlusion method. L(p) measured after a 15-minute perfusion with Ringer's albumin solution (control) was compared with L(p) after a subsequent 15-minute perfusion with one of three treatments: control (n = 4), 8 pM ET-1 (n = 6), or 80 pM ET-1 (n = 6). Results: Baseline L(p) for all vessels averaged (± SE) 8.1 ± 0.8 x 10-8 cm · sec-1 · cm H2O-1 and was not significantly different between groups. Perfusion with either control or 8 pM ET-1 did not significantly change the L(p) of any of the vessels. Significant decreases in L(p) of 40 to 60% were observed in venules perfused with 80 pM ET-1. The average L(p) in this group was 9.9 ± 1.4 during baseline and decreased to 5.0 ± 0.7 during ET-1 perfusion (p = 0.003). Washout of 80 pM ET-1 for periods of up to 15 minutes did not return L(p) to baseline values. Conclusion: Low-dose ET-1 did not directly increase Lp in postcapillary venules. ET-1 at 80 pM, however, significantly decreased L(p). These data implicate factors other than a direct permeability-increasing effect in ET-1. At higher concentrations, ET-1 may have a protective effect on endothelial barrier function.
AB - Background: There is evidence that endothelin-1 (ET-1) increases extravasation of fluid and protein into vascular beds. The present study was designed to determine the direct effects of ET-1 on hydraulic permeability (L(p)) when microvascular hydraulic and oncotic pressures are controlled. Methods: Postcapillary venules in the rat mesentery were perfused in situ and paired measurements of L(p) obtained by using the modified Landis micro-occlusion method. L(p) measured after a 15-minute perfusion with Ringer's albumin solution (control) was compared with L(p) after a subsequent 15-minute perfusion with one of three treatments: control (n = 4), 8 pM ET-1 (n = 6), or 80 pM ET-1 (n = 6). Results: Baseline L(p) for all vessels averaged (± SE) 8.1 ± 0.8 x 10-8 cm · sec-1 · cm H2O-1 and was not significantly different between groups. Perfusion with either control or 8 pM ET-1 did not significantly change the L(p) of any of the vessels. Significant decreases in L(p) of 40 to 60% were observed in venules perfused with 80 pM ET-1. The average L(p) in this group was 9.9 ± 1.4 during baseline and decreased to 5.0 ± 0.7 during ET-1 perfusion (p = 0.003). Washout of 80 pM ET-1 for periods of up to 15 minutes did not return L(p) to baseline values. Conclusion: Low-dose ET-1 did not directly increase Lp in postcapillary venules. ET-1 at 80 pM, however, significantly decreased L(p). These data implicate factors other than a direct permeability-increasing effect in ET-1. At higher concentrations, ET-1 may have a protective effect on endothelial barrier function.
KW - Fluid filtration
KW - Landis micro-occlusion technique
KW - Mesentery
KW - Microvascular permeability
KW - Postcapillary venules
UR - http://www.scopus.com/inward/record.url?scp=0032882137&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032882137&partnerID=8YFLogxK
U2 - 10.1097/00005373-199910000-00016
DO - 10.1097/00005373-199910000-00016
M3 - Article
C2 - 10528606
AN - SCOPUS:0032882137
VL - 47
SP - 713
EP - 718
JO - Journal of Trauma and Acute Care Surgery
JF - Journal of Trauma and Acute Care Surgery
SN - 2163-0755
IS - 4
ER -