Differing intrinsic biological properties between forebrain and spinal oligodendroglial lineage cells

Makoto Horiuchi, Yoko Suzuki-Horiuchi, Tasuku Akiyama, Aki Itoh, David E Pleasure, Earl Carstens, Takayuki Ito

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Differentiation of oligodendroglial progenitor cells (OPCs) into myelinating oligodendrocytes is known to be regulated by the microenvironment where they differentiate. However, current research has not verified whether or not oligodendroglial lineage cells (OLCs) derived from different anatomical regions of the central nervous system (CNS) respond to microenvironmental cues in the same manner. Here, we isolated pure OPCs from rat neonatal forebrain (FB) and spinal cord (SC) and compared their phenotypes in the same in vitro conditions. We found that although FB and SC OLCs responded differently to the same external factors; they were distinct in proliferation response to mitogens, oligodendrocyte phenotype after differentiation, and cytotoxic responses to α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate-type glutamate receptor-mediated excitotoxicity at immature stages of differentiation in a cell-intrinsic manner. Moreover, transcriptome analysis identified genes differentially expressed between these OPC populations, including those encoding transcription factors (TFs), cell surface molecules, and signaling molecules. Particularly, FB and SC OPCs retained the expression of FB- or SC-specific TFs, such as Foxg1 and Hoxc8, respectively, even after serial passaging in vitro. Given the essential role of these TFs in the regional identities of CNS cells along the rostrocaudal axis, our results suggest that CNS region-specific gene regulation by these TFs may cause cell-intrinsic differences in cellular responses between FB and SC OLCs to extracellular molecules. Further understanding of the regional differences among OPC populations will help to improve treatments for demyelination in different CNS regions and to facilitate the development of stem cell-derived OPCs for cell transplantation therapies for demyelination. (Figure presented.). Cover Image for this issue: doi. 10.1111/jnc.13809.

Original languageEnglish (US)
Pages (from-to)378-391
Number of pages14
JournalJournal of Neurochemistry
Volume142
Issue number3
DOIs
StatePublished - Aug 1 2017

Fingerprint

Prosencephalon
Neurology
Stem Cells
Transcription Factors
Spinal Cord
Central Nervous System
Molecules
Oligodendroglia
Demyelinating Diseases
Cells
Glutamate Receptors
Stem cells
Mitogens
Gene expression
Phenotype
Rats
Cell Transplantation
Gene Expression Profiling
Genes
Cell- and Tissue-Based Therapy

Keywords

  • differentiation
  • excitotoxicity
  • Hox gene
  • proliferation
  • rostrocaudal axis

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Differing intrinsic biological properties between forebrain and spinal oligodendroglial lineage cells. / Horiuchi, Makoto; Suzuki-Horiuchi, Yoko; Akiyama, Tasuku; Itoh, Aki; Pleasure, David E; Carstens, Earl; Ito, Takayuki.

In: Journal of Neurochemistry, Vol. 142, No. 3, 01.08.2017, p. 378-391.

Research output: Contribution to journalArticle

Horiuchi, M, Suzuki-Horiuchi, Y, Akiyama, T, Itoh, A, Pleasure, DE, Carstens, E & Ito, T 2017, 'Differing intrinsic biological properties between forebrain and spinal oligodendroglial lineage cells', Journal of Neurochemistry, vol. 142, no. 3, pp. 378-391. https://doi.org/10.1111/jnc.14074
Horiuchi, Makoto ; Suzuki-Horiuchi, Yoko ; Akiyama, Tasuku ; Itoh, Aki ; Pleasure, David E ; Carstens, Earl ; Ito, Takayuki. / Differing intrinsic biological properties between forebrain and spinal oligodendroglial lineage cells. In: Journal of Neurochemistry. 2017 ; Vol. 142, No. 3. pp. 378-391.
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