Abstract
NG2 chondroitin sulfate proteoglycan is a surface marker of oligodendroglial progenitor cells (OPCs) in various species. In contrast to well-studied rat OPCs, however, we found that purified mouse NG2 surface positive cells (NG2+ cells) require additional activation of cyclic AMP (cAMP) signaling for survival in a medium containing 30% B104 neuroblastoma conditioned medium supplemented with fibroblast growth factor-2 (B104CM+FGF2), whereas B104CM+FGF2 alone is sufficient for survival and selective proliferation of rat OPCs. After induction of in vitro differentiation, more than 90% of mouse NG2+ cells became O4-positive, and a majority expressed myelin basic protein by 5 day of differentiation, which confirmed the identity of isolated mouse NG2+ cells as OPCs. In comparison to rat OPCs, mouse OPCs in B104CM+FGF2 were less motile, and demonstrated lower basal phosphorylation levels of ERK1/2 and cAMP response element-binding protein (CREB) and a higher incidence of apoptosis mediated by the intrinsic pathway. Transient up-regulation of cAMP-CREB signaling partially inhibited apoptosis of mouse OPCs independently of the ERK pathway. This study demonstrates a difference in trophic requirements between mouse and rat OPCs, with an essential role for cAMP signaling to preserve viability of mouse OPCs.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 957-970 |
| Number of pages | 14 |
| Journal | Journal of Neuroscience Research |
| Volume | 88 |
| Issue number | 5 |
| DOIs | |
| State | Published - Apr 2010 |
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Keywords
- Cyclic AMP response element-binding protein
- Cyclic AMP signaling
- Extracellular signal-regulated kinase
- Mouse oligodendroglial progenitor cell
- NG2 chondroitine sulfate proteoglycan
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience
Cite this
Differing in vitro survival dependency of mouse and rat NG2+ oligodendroglial progenitor cells. / Horiuchi, Makoto; Lindsten, Tullia; Pleasure, David E; Ito, Takayuki.
In: Journal of Neuroscience Research, Vol. 88, No. 5, 04.2010, p. 957-970.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Differing in vitro survival dependency of mouse and rat NG2+ oligodendroglial progenitor cells
AU - Horiuchi, Makoto
AU - Lindsten, Tullia
AU - Pleasure, David E
AU - Ito, Takayuki
PY - 2010/4
Y1 - 2010/4
N2 - NG2 chondroitin sulfate proteoglycan is a surface marker of oligodendroglial progenitor cells (OPCs) in various species. In contrast to well-studied rat OPCs, however, we found that purified mouse NG2 surface positive cells (NG2+ cells) require additional activation of cyclic AMP (cAMP) signaling for survival in a medium containing 30% B104 neuroblastoma conditioned medium supplemented with fibroblast growth factor-2 (B104CM+FGF2), whereas B104CM+FGF2 alone is sufficient for survival and selective proliferation of rat OPCs. After induction of in vitro differentiation, more than 90% of mouse NG2+ cells became O4-positive, and a majority expressed myelin basic protein by 5 day of differentiation, which confirmed the identity of isolated mouse NG2+ cells as OPCs. In comparison to rat OPCs, mouse OPCs in B104CM+FGF2 were less motile, and demonstrated lower basal phosphorylation levels of ERK1/2 and cAMP response element-binding protein (CREB) and a higher incidence of apoptosis mediated by the intrinsic pathway. Transient up-regulation of cAMP-CREB signaling partially inhibited apoptosis of mouse OPCs independently of the ERK pathway. This study demonstrates a difference in trophic requirements between mouse and rat OPCs, with an essential role for cAMP signaling to preserve viability of mouse OPCs.
AB - NG2 chondroitin sulfate proteoglycan is a surface marker of oligodendroglial progenitor cells (OPCs) in various species. In contrast to well-studied rat OPCs, however, we found that purified mouse NG2 surface positive cells (NG2+ cells) require additional activation of cyclic AMP (cAMP) signaling for survival in a medium containing 30% B104 neuroblastoma conditioned medium supplemented with fibroblast growth factor-2 (B104CM+FGF2), whereas B104CM+FGF2 alone is sufficient for survival and selective proliferation of rat OPCs. After induction of in vitro differentiation, more than 90% of mouse NG2+ cells became O4-positive, and a majority expressed myelin basic protein by 5 day of differentiation, which confirmed the identity of isolated mouse NG2+ cells as OPCs. In comparison to rat OPCs, mouse OPCs in B104CM+FGF2 were less motile, and demonstrated lower basal phosphorylation levels of ERK1/2 and cAMP response element-binding protein (CREB) and a higher incidence of apoptosis mediated by the intrinsic pathway. Transient up-regulation of cAMP-CREB signaling partially inhibited apoptosis of mouse OPCs independently of the ERK pathway. This study demonstrates a difference in trophic requirements between mouse and rat OPCs, with an essential role for cAMP signaling to preserve viability of mouse OPCs.
KW - Cyclic AMP response element-binding protein
KW - Cyclic AMP signaling
KW - Extracellular signal-regulated kinase
KW - Mouse oligodendroglial progenitor cell
KW - NG2 chondroitine sulfate proteoglycan
UR - http://www.scopus.com/inward/record.url?scp=77950146306&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77950146306&partnerID=8YFLogxK
U2 - 10.1002/jnr.22262
DO - 10.1002/jnr.22262
M3 - Article
C2 - 19908280
AN - SCOPUS:77950146306
VL - 88
SP - 957
EP - 970
JO - Journal of Neuroscience Research
JF - Journal of Neuroscience Research
SN - 0360-4012
IS - 5
ER -