Differing in vitro survival dependency of mouse and rat NG2+ oligodendroglial progenitor cells

Makoto Horiuchi, Tullia Lindsten, David E Pleasure, Takayuki Ito

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

NG2 chondroitin sulfate proteoglycan is a surface marker of oligodendroglial progenitor cells (OPCs) in various species. In contrast to well-studied rat OPCs, however, we found that purified mouse NG2 surface positive cells (NG2+ cells) require additional activation of cyclic AMP (cAMP) signaling for survival in a medium containing 30% B104 neuroblastoma conditioned medium supplemented with fibroblast growth factor-2 (B104CM+FGF2), whereas B104CM+FGF2 alone is sufficient for survival and selective proliferation of rat OPCs. After induction of in vitro differentiation, more than 90% of mouse NG2+ cells became O4-positive, and a majority expressed myelin basic protein by 5 day of differentiation, which confirmed the identity of isolated mouse NG2+ cells as OPCs. In comparison to rat OPCs, mouse OPCs in B104CM+FGF2 were less motile, and demonstrated lower basal phosphorylation levels of ERK1/2 and cAMP response element-binding protein (CREB) and a higher incidence of apoptosis mediated by the intrinsic pathway. Transient up-regulation of cAMP-CREB signaling partially inhibited apoptosis of mouse OPCs independently of the ERK pathway. This study demonstrates a difference in trophic requirements between mouse and rat OPCs, with an essential role for cAMP signaling to preserve viability of mouse OPCs.

Original languageEnglish (US)
Pages (from-to)957-970
Number of pages14
JournalJournal of Neuroscience Research
Volume88
Issue number5
DOIs
StatePublished - Apr 2010

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Stem Cells
Fibroblast Growth Factor 2
Cyclic AMP Response Element-Binding Protein
Cyclic AMP
Apoptosis
Chondroitin Sulfate Proteoglycans
In Vitro Techniques
Myelin Basic Protein
MAP Kinase Signaling System
Conditioned Culture Medium
Neuroblastoma
Up-Regulation
Phosphorylation
Incidence

Keywords

  • Cyclic AMP response element-binding protein
  • Cyclic AMP signaling
  • Extracellular signal-regulated kinase
  • Mouse oligodendroglial progenitor cell
  • NG2 chondroitine sulfate proteoglycan

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Differing in vitro survival dependency of mouse and rat NG2+ oligodendroglial progenitor cells. / Horiuchi, Makoto; Lindsten, Tullia; Pleasure, David E; Ito, Takayuki.

In: Journal of Neuroscience Research, Vol. 88, No. 5, 04.2010, p. 957-970.

Research output: Contribution to journalArticle

Horiuchi, Makoto ; Lindsten, Tullia ; Pleasure, David E ; Ito, Takayuki. / Differing in vitro survival dependency of mouse and rat NG2+ oligodendroglial progenitor cells. In: Journal of Neuroscience Research. 2010 ; Vol. 88, No. 5. pp. 957-970.
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