Differentiation of strains of equine arteritis virus of differing virulence to horses by growth in equine endothelial cells

Brian D. Moore, Udeni B R Balasuriya, Jane P. Nurton, William H. McCollum, Peter J. Timoney, Alan J. Guthrie, Nigel J Maclachlan

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Objective - To compare growth characteristics of strains of equine arteritis virus (EAV) of differing virulence to horses in rabbit kidney (RK)-13 cells and equine endothelial cells (EECs) cultured from the pulmonary artery of a foal. Sample Population - 13 strains of EAV, including 11 field isolates of differing virulence to horses; the highly virulent, horse-adapted Bucyrus strain; and the modified-live virus (MLV) vaccine derived from it. Procedure - The growth characteristics of the 13 strains were compared in EECs and RK-13 cells. Viral nucleoprotein expression, cytopathogenicity, and plaque size were compared to determine whether growth characteristics of the 13 strains were predictive of their virulence to horses. Results - Cytopathogenicity, viral nucleoprotein expression, and plaque size induced by all 13 viruses were similar in RK-13 cells, whereas virulent strains of EAV caused significantly larger plaques in EECs than did the avirulent strains of EAV. Paradoxically, the highly attenuated MLV vaccine and 1 field isolate of EAV caused plaques in EECs that were larger than those caused by any of the other viruses, and sequence analysis confirmed the field isolate of EAV to be indistinguishable from the MLV vaccine. Conclusions and Clinical Relevance - With the notable exception of the MLV vaccine, growth of the various strains of EAV in EECs was predictive of their individual virulence to horses. Thus, EECs provide a relevant and useful model to further characterize determinants of virulence and attenuation amongst strains of EAV.

Original languageEnglish (US)
Pages (from-to)779-784
Number of pages6
JournalAmerican Journal of Veterinary Research
Volume64
Issue number6
DOIs
StatePublished - Jun 1 2003

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Equine Arteritis Virus
Equine arteritis virus
endothelial cells
Horses
Virulence
virulence
Endothelial Cells
horses
Growth
Viruses
viruses
Vaccines
vaccines
nucleoproteins
Nucleoproteins
cytopathogenicity
rabbits
kidneys
Rabbits
Kidney

ASJC Scopus subject areas

  • veterinary(all)

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Differentiation of strains of equine arteritis virus of differing virulence to horses by growth in equine endothelial cells. / Moore, Brian D.; Balasuriya, Udeni B R; Nurton, Jane P.; McCollum, William H.; Timoney, Peter J.; Guthrie, Alan J.; Maclachlan, Nigel J.

In: American Journal of Veterinary Research, Vol. 64, No. 6, 01.06.2003, p. 779-784.

Research output: Contribution to journalArticle

Moore, Brian D. ; Balasuriya, Udeni B R ; Nurton, Jane P. ; McCollum, William H. ; Timoney, Peter J. ; Guthrie, Alan J. ; Maclachlan, Nigel J. / Differentiation of strains of equine arteritis virus of differing virulence to horses by growth in equine endothelial cells. In: American Journal of Veterinary Research. 2003 ; Vol. 64, No. 6. pp. 779-784.
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