Differentiation of human fetal osteoblastic cells and gap junctional intercellular communication

Henry J. Donahue, Zhongyong Li, Zhiyi Zhou, Clare E Yellowley-genetos

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

Gap junctional channels facilitate intercellular communication and in doing so may contribute to cellular differentiation. To test this hypothesis, we examined gap junction expression and function in a temperature-sensitive human fetal osteoblastic cell line (hFOB 1.19) that when cultured at 37°C proliferates rapidly but when cultured at 39.5°C proliferates slowly and displays increased alkaline phosphatase activity and osteocalcin synthesis. We found that hFOB 1.19 cells express abundant connexin 43 (Cx43) protein and mRNA. In contrast, Cx45 mRNA was expressed to a lesser degree, and Cx26 and Cx32 mRNA were not detected. Culturing hFOB 1.19 cells at 39.5°C, relative to 37°C, inhibited proliferation, increased Cx43 mRNA and protein expression, and increased gap junctional intercellular communication (GJIC). Blocking GJIC with 18α-glycyrrhetinic acid prevented the increase in alkaline phosphatase activity resulting from culture at 39.5°C but did not affect osteocalcin levels. These results suggest that gap junction function and expression parallel osteoblastic differentiation and contribute to the expression of alkaline phosphatase activity, a marker for fully differentiated osteoblastic cells.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume278
Issue number2 47-2
StatePublished - 2000
Externally publishedYes

Fingerprint

Cells
Alkaline Phosphatase
Messenger RNA
Connexin 43
Gap Junctions
Communication
Osteocalcin
Glycyrrhetinic Acid
Proteins
Display devices
Cell Line
Temperature

Keywords

  • Alkaline phosphatase
  • Bone
  • Connexin
  • Osteocalcin
  • Proliferation

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology
  • Physiology (medical)

Cite this

Differentiation of human fetal osteoblastic cells and gap junctional intercellular communication. / Donahue, Henry J.; Li, Zhongyong; Zhou, Zhiyi; Yellowley-genetos, Clare E.

In: American Journal of Physiology - Cell Physiology, Vol. 278, No. 2 47-2, 2000.

Research output: Contribution to journalArticle

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