Differentiation of canalicular cell processes in bone cells by basement membrane matrix components: Regulation by discrete domains of laminin

Slobodan Vukicevic, Frank P. Luyten, Hynda K. Kleinman, A Hari Reddi

Research output: Contribution to journalArticlepeer-review

156 Scopus citations

Abstract

We have investigated the interaction of rat primary calvarial bone cells and a mouse osteoblast-like cell line MC3T3-E1 with basement membrane components. On a reconstituted gel of basement membrane, both cell types attached and formed isolated clusters that developed long interconnecting cell processes similar to the canalicular network observed in bone. The differentiation of the osteoblastic phenotype was stimulated as determined by increased alkaline phosphatase production and the deposition of mineral. Antibodies to laminin and to a 32 67 kd laminin receptor blocked this differentiation. Cell morphology was altered by the addition of active laminin-derived synthetic peptides, YIGSR-NH2 and CSRARKQAASIKVAVSADR-NH2, but not by an active RGD-containing peptide. When coated directly on plastic, all three peptides promoted cell adhesion, demonstrating that bone cells interact with specific molecular domains of laminin. These data demonstrate that basement membrane plays a key role in formation of a network of cytoplasmic processes resembling the osteocyte canalicular network in bone.

Original languageEnglish (US)
Pages (from-to)437-445
Number of pages9
JournalCell
Volume63
Issue number2
DOIs
StatePublished - Oct 19 1990
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

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