Differentiation from thymic B cell progenitors to mature B cells in vitro

Akira Sugihara, Muneo Inaba, Shin Ichiro Mori, Shigeru Taketani, Yasushi Adachi, Hiroko Hisha, Kayo Inaba, Junko Toki, Takeshi Horio, M. Eric Gershwin, Susumu Ikehara

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6 Scopus citations

Abstract

The role of the thymic microenvironment in the development of murine thymic B cells has yet to be fully clarified. We therefore investigate the microenvironment that supports the development of mature thymic B cells (sIg+/B220+CD43-B cells) from thymic B cell progenitors with immunophenotypes of sIg-/B220(med)/CD43+ cells. As we have previously reported, thymic B cells generated from these progenitors in the thymus are CD5+ B cells. We next study the in vitro condition that supports the differentiation of thymic B cell progenitors. Stromal cells (from the bone marrow or thymus), thymus-derived cell lines with the character of thymic nurse cells (TNCs) or thymic epithelial cells (TECs), or the bone marrow-derived cell line (MS-5) are tested for their ability to support B-lymphopoiesis from thymic B cell progenitors. Interestingly, thymic stromal cells (but neither stromal cells from the bone marrow nor stromal cell lines) support the differentiation of thymic B cell progenitors into thymic B cells in the presence of IL-7. Cortical epithelia (but not medullary epithelia, thymic macrophages or dendritic cells) are found to contribute to thymic B cell differentiation. Surface phenotype and Ig rearrangement analyses reveal that mature B cells generated in this condition are primarily CD5+ B cells, indicating that the thymic microenvironment (particularly cortical epithelia) determines the differentiation of thymic B cells.

Original languageEnglish (US)
Pages (from-to)515-526
Number of pages12
JournalImmunobiology
Volume201
Issue number5
StatePublished - Apr 2000
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology

Cite this

Sugihara, A., Inaba, M., Mori, S. I., Taketani, S., Adachi, Y., Hisha, H., Inaba, K., Toki, J., Horio, T., Gershwin, M. E., & Ikehara, S. (2000). Differentiation from thymic B cell progenitors to mature B cells in vitro. Immunobiology, 201(5), 515-526.