Differentiation from thymic B cell progenitors to mature B cells in vitro

Akira Sugihara, Muneo Inaba, Shin Ichiro Mori, Shigeru Taketani, Yasushi Adachi, Hiroko Hisha, Kayo Inaba, Junko Toki, Takeshi Horio, M. Eric Gershwin, Susumu Ikehara

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The role of the thymic microenvironment in the development of murine thymic B cells has yet to be fully clarified. We therefore investigate the microenvironment that supports the development of mature thymic B cells (sIg+/B220+CD43-B cells) from thymic B cell progenitors with immunophenotypes of sIg-/B220(med)/CD43+ cells. As we have previously reported, thymic B cells generated from these progenitors in the thymus are CD5+ B cells. We next study the in vitro condition that supports the differentiation of thymic B cell progenitors. Stromal cells (from the bone marrow or thymus), thymus-derived cell lines with the character of thymic nurse cells (TNCs) or thymic epithelial cells (TECs), or the bone marrow-derived cell line (MS-5) are tested for their ability to support B-lymphopoiesis from thymic B cell progenitors. Interestingly, thymic stromal cells (but neither stromal cells from the bone marrow nor stromal cell lines) support the differentiation of thymic B cell progenitors into thymic B cells in the presence of IL-7. Cortical epithelia (but not medullary epithelia, thymic macrophages or dendritic cells) are found to contribute to thymic B cell differentiation. Surface phenotype and Ig rearrangement analyses reveal that mature B cells generated in this condition are primarily CD5+ B cells, indicating that the thymic microenvironment (particularly cortical epithelia) determines the differentiation of thymic B cells.

Original languageEnglish (US)
Pages (from-to)515-526
Number of pages12
JournalImmunobiology
Volume201
Issue number5
StatePublished - Apr 2000
Externally publishedYes

Fingerprint

B-Lymphoid Precursor Cells
B-Lymphocytes
Thymus Gland
Epithelium
Stromal Cells
Mesenchymal Stromal Cells
Cell Line
Lymphopoiesis
In Vitro Techniques
Interleukin-7
Bone Marrow Cells
Dendritic Cells
Cell Differentiation
Epithelial Cells
Macrophages
Nurses
Phenotype

ASJC Scopus subject areas

  • Immunology

Cite this

Sugihara, A., Inaba, M., Mori, S. I., Taketani, S., Adachi, Y., Hisha, H., ... Ikehara, S. (2000). Differentiation from thymic B cell progenitors to mature B cells in vitro. Immunobiology, 201(5), 515-526.

Differentiation from thymic B cell progenitors to mature B cells in vitro. / Sugihara, Akira; Inaba, Muneo; Mori, Shin Ichiro; Taketani, Shigeru; Adachi, Yasushi; Hisha, Hiroko; Inaba, Kayo; Toki, Junko; Horio, Takeshi; Gershwin, M. Eric; Ikehara, Susumu.

In: Immunobiology, Vol. 201, No. 5, 04.2000, p. 515-526.

Research output: Contribution to journalArticle

Sugihara, A, Inaba, M, Mori, SI, Taketani, S, Adachi, Y, Hisha, H, Inaba, K, Toki, J, Horio, T, Gershwin, ME & Ikehara, S 2000, 'Differentiation from thymic B cell progenitors to mature B cells in vitro', Immunobiology, vol. 201, no. 5, pp. 515-526.
Sugihara A, Inaba M, Mori SI, Taketani S, Adachi Y, Hisha H et al. Differentiation from thymic B cell progenitors to mature B cells in vitro. Immunobiology. 2000 Apr;201(5):515-526.
Sugihara, Akira ; Inaba, Muneo ; Mori, Shin Ichiro ; Taketani, Shigeru ; Adachi, Yasushi ; Hisha, Hiroko ; Inaba, Kayo ; Toki, Junko ; Horio, Takeshi ; Gershwin, M. Eric ; Ikehara, Susumu. / Differentiation from thymic B cell progenitors to mature B cells in vitro. In: Immunobiology. 2000 ; Vol. 201, No. 5. pp. 515-526.
@article{6892981675934df58e24a5966efd5dfc,
title = "Differentiation from thymic B cell progenitors to mature B cells in vitro",
abstract = "The role of the thymic microenvironment in the development of murine thymic B cells has yet to be fully clarified. We therefore investigate the microenvironment that supports the development of mature thymic B cells (sIg+/B220+CD43-B cells) from thymic B cell progenitors with immunophenotypes of sIg-/B220(med)/CD43+ cells. As we have previously reported, thymic B cells generated from these progenitors in the thymus are CD5+ B cells. We next study the in vitro condition that supports the differentiation of thymic B cell progenitors. Stromal cells (from the bone marrow or thymus), thymus-derived cell lines with the character of thymic nurse cells (TNCs) or thymic epithelial cells (TECs), or the bone marrow-derived cell line (MS-5) are tested for their ability to support B-lymphopoiesis from thymic B cell progenitors. Interestingly, thymic stromal cells (but neither stromal cells from the bone marrow nor stromal cell lines) support the differentiation of thymic B cell progenitors into thymic B cells in the presence of IL-7. Cortical epithelia (but not medullary epithelia, thymic macrophages or dendritic cells) are found to contribute to thymic B cell differentiation. Surface phenotype and Ig rearrangement analyses reveal that mature B cells generated in this condition are primarily CD5+ B cells, indicating that the thymic microenvironment (particularly cortical epithelia) determines the differentiation of thymic B cells.",
author = "Akira Sugihara and Muneo Inaba and Mori, {Shin Ichiro} and Shigeru Taketani and Yasushi Adachi and Hiroko Hisha and Kayo Inaba and Junko Toki and Takeshi Horio and Gershwin, {M. Eric} and Susumu Ikehara",
year = "2000",
month = "4",
language = "English (US)",
volume = "201",
pages = "515--526",
journal = "Immunobiology",
issn = "0171-2985",
publisher = "Urban und Fischer Verlag GmbH und Co. KG",
number = "5",

}

TY - JOUR

T1 - Differentiation from thymic B cell progenitors to mature B cells in vitro

AU - Sugihara, Akira

AU - Inaba, Muneo

AU - Mori, Shin Ichiro

AU - Taketani, Shigeru

AU - Adachi, Yasushi

AU - Hisha, Hiroko

AU - Inaba, Kayo

AU - Toki, Junko

AU - Horio, Takeshi

AU - Gershwin, M. Eric

AU - Ikehara, Susumu

PY - 2000/4

Y1 - 2000/4

N2 - The role of the thymic microenvironment in the development of murine thymic B cells has yet to be fully clarified. We therefore investigate the microenvironment that supports the development of mature thymic B cells (sIg+/B220+CD43-B cells) from thymic B cell progenitors with immunophenotypes of sIg-/B220(med)/CD43+ cells. As we have previously reported, thymic B cells generated from these progenitors in the thymus are CD5+ B cells. We next study the in vitro condition that supports the differentiation of thymic B cell progenitors. Stromal cells (from the bone marrow or thymus), thymus-derived cell lines with the character of thymic nurse cells (TNCs) or thymic epithelial cells (TECs), or the bone marrow-derived cell line (MS-5) are tested for their ability to support B-lymphopoiesis from thymic B cell progenitors. Interestingly, thymic stromal cells (but neither stromal cells from the bone marrow nor stromal cell lines) support the differentiation of thymic B cell progenitors into thymic B cells in the presence of IL-7. Cortical epithelia (but not medullary epithelia, thymic macrophages or dendritic cells) are found to contribute to thymic B cell differentiation. Surface phenotype and Ig rearrangement analyses reveal that mature B cells generated in this condition are primarily CD5+ B cells, indicating that the thymic microenvironment (particularly cortical epithelia) determines the differentiation of thymic B cells.

AB - The role of the thymic microenvironment in the development of murine thymic B cells has yet to be fully clarified. We therefore investigate the microenvironment that supports the development of mature thymic B cells (sIg+/B220+CD43-B cells) from thymic B cell progenitors with immunophenotypes of sIg-/B220(med)/CD43+ cells. As we have previously reported, thymic B cells generated from these progenitors in the thymus are CD5+ B cells. We next study the in vitro condition that supports the differentiation of thymic B cell progenitors. Stromal cells (from the bone marrow or thymus), thymus-derived cell lines with the character of thymic nurse cells (TNCs) or thymic epithelial cells (TECs), or the bone marrow-derived cell line (MS-5) are tested for their ability to support B-lymphopoiesis from thymic B cell progenitors. Interestingly, thymic stromal cells (but neither stromal cells from the bone marrow nor stromal cell lines) support the differentiation of thymic B cell progenitors into thymic B cells in the presence of IL-7. Cortical epithelia (but not medullary epithelia, thymic macrophages or dendritic cells) are found to contribute to thymic B cell differentiation. Surface phenotype and Ig rearrangement analyses reveal that mature B cells generated in this condition are primarily CD5+ B cells, indicating that the thymic microenvironment (particularly cortical epithelia) determines the differentiation of thymic B cells.

UR - http://www.scopus.com/inward/record.url?scp=0034009688&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034009688&partnerID=8YFLogxK

M3 - Article

C2 - 10834310

AN - SCOPUS:0034009688

VL - 201

SP - 515

EP - 526

JO - Immunobiology

JF - Immunobiology

SN - 0171-2985

IS - 5

ER -