Differentiating Inbred Mouse Strains from Each Other and Those with Single Gene Mutations Using Hair Proteomics

Robert H. Rice, Katie M. Bradshaw, Blythe P. Durbin-Johnson, David M Rocke, Richard A. Eigenheer, Brett S. Phinney, John P. Sundberg

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Mutant laboratory mice with distinctive hair phenotypes are useful for identifying genes responsible for hair diseases. The work presented here demonstrates that shotgun proteomic profiling can distinguish hair shafts from different inbred mouse strains. For this purpose, analyzing the total hair shaft provided better discrimination than analyzing the isolated solubilized and particulate (cross-linked) fractions. Over 100 proteins exhibited significant differences among the 11 strains and 5 mutant stocks across the wide spectrum of strains surveyed. Effects on the profile of single gene mutations causing hair shaft defects were profound. Since the hair shaft provides a discrete sampling of the species proteome, with constituents serving important functions in epidermal appendages and throughout the body, this work provides a foundation for non-invasive diagnosis of genetic diseases of hair and perhaps other tissues.

Original languageEnglish (US)
Article numbere51956
JournalPLoS One
Volume7
Issue number12
DOIs
StatePublished - Dec 14 2012

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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    Rice, R. H., Bradshaw, K. M., Durbin-Johnson, B. P., Rocke, D. M., Eigenheer, R. A., Phinney, B. S., & Sundberg, J. P. (2012). Differentiating Inbred Mouse Strains from Each Other and Those with Single Gene Mutations Using Hair Proteomics. PLoS One, 7(12), [e51956]. https://doi.org/10.1371/journal.pone.0051956