Differentiated structure and function of primary cultures of monkey oviductal epithelium

M. Rajagopal, Theodore L Tollner, W. E. Finkbeiner, G. N. Cherr, Jonathan Widdicombe

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

We have established well-differentiated, polarized cultures of monkey oviductal epithelium. Oviductal epithelial cells were isolated by protease digestion and plated on collagen-coated, porous cell culture inserts. About 5 d after plating, cells developed detectable transepithelial electrical resistance of up to 2000 Ω.cm2 (an index of tight junction formation) and transepithelial voltages of up to 20 mV (an index of vectorial transepithelial ion transport). Measurements of short-circuit current in Ussing chambers indicated that active secretion of Cl was the major transepithelial active ion transport process, and that this was stimulated by elevation of either cAMP or Cai. Furthermore, estimates of the volume of mucosal liquid were consistent with Cl secretion mediating fluid secretion. Various microscopical methods showed that the cultures were densely ciliated and contained mature secretory cells. Transport across the oviductal epithelium determines the composition of the oviductal fluid, and the study of the relevant transport processes will be greatly enhanced by well-differentiated cultures of oviductal epithelium of the kind established here.

Original languageEnglish (US)
Pages (from-to)248-254
Number of pages7
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume42
Issue number8-9
StatePublished - Sep 2006

Keywords

  • Chloride secretion
  • Purinergic agonists
  • Short-circuit current
  • Transepithelial electrical resistance

ASJC Scopus subject areas

  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology

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