Differentially expressed circRNAs in melanocytes and melanoma cells and their effect on cell proliferation and invasion

Qi Wang, Jia Chen, Aijun Wang, Lichun Sun, Li Qian, Xiao Zhou, Yu Liu, Shijie Tang, Xiang Chen, Yan Cheng, Ke Cao, Jianda Zhou

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Circular RNAs (circRNAs) play critical roles in the occurrence of human diseases, including cancer. However, the detailed functions of circRNAs in melanoma have not been fully elucidated. In the present study, a circRNA microarray was performed to analyze the variability of circRNAs in the low-metastatic melanoma WM35 cell line and in the high-metastatic melanoma WM451 cell line in comparison to control human melanocytes. The results revealed that five circRNAs were upregulated and four circRNAs were downregulated in both the WM35 and WM451 cells. qRT-PCR revealed an upregulated expression of circ0000082 and circ0016418 and a downregulation of circ0023988, circ0008157 and circ0030388 in the cells which was consistent with the results of the microarray assay. Functional tests revealed that knockdown of circ0023988, circ0008157 or circ0030388 significantly promoted the proliferation and invasion of the WM35 cells. Following the silencing of circ0000082 or circ0016418 in WM451 cells, the proliferation and invasion of the WM451 cells were inhibited. Bioinformatic analysis predicted that the circ0000082-, circ0023988-and circ0008157-circRNAmiRNA-mRNA network may participate in the occurrence, development, invasion and metastasis of malignant tumors. The present study revealed several differentially expressed circRNAs, indicating that the newly identified circRNAs may provide new therapeutic targets for melanoma.

Original languageEnglish (US)
Pages (from-to)1813-1824
Number of pages12
JournalOncology Reports
Volume39
Issue number4
DOIs
StatePublished - Apr 1 2018

Fingerprint

Melanocytes
Melanoma
Cell Proliferation
Down-Regulation
Cell Line
circular RNA
Computational Biology
Neoplasms
Neoplasm Metastasis
Polymerase Chain Reaction
Messenger RNA

Keywords

  • CeRNA
  • Circular RNA
  • Melanoma
  • MiRNA
  • SiRNA

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Differentially expressed circRNAs in melanocytes and melanoma cells and their effect on cell proliferation and invasion. / Wang, Qi; Chen, Jia; Wang, Aijun; Sun, Lichun; Qian, Li; Zhou, Xiao; Liu, Yu; Tang, Shijie; Chen, Xiang; Cheng, Yan; Cao, Ke; Zhou, Jianda.

In: Oncology Reports, Vol. 39, No. 4, 01.04.2018, p. 1813-1824.

Research output: Contribution to journalArticle

Wang, Q, Chen, J, Wang, A, Sun, L, Qian, L, Zhou, X, Liu, Y, Tang, S, Chen, X, Cheng, Y, Cao, K & Zhou, J 2018, 'Differentially expressed circRNAs in melanocytes and melanoma cells and their effect on cell proliferation and invasion', Oncology Reports, vol. 39, no. 4, pp. 1813-1824. https://doi.org/10.3892/or.2018.6263
Wang, Qi ; Chen, Jia ; Wang, Aijun ; Sun, Lichun ; Qian, Li ; Zhou, Xiao ; Liu, Yu ; Tang, Shijie ; Chen, Xiang ; Cheng, Yan ; Cao, Ke ; Zhou, Jianda. / Differentially expressed circRNAs in melanocytes and melanoma cells and their effect on cell proliferation and invasion. In: Oncology Reports. 2018 ; Vol. 39, No. 4. pp. 1813-1824.
@article{bef6627ea6c242cf8d9e5460397713d1,
title = "Differentially expressed circRNAs in melanocytes and melanoma cells and their effect on cell proliferation and invasion",
abstract = "Circular RNAs (circRNAs) play critical roles in the occurrence of human diseases, including cancer. However, the detailed functions of circRNAs in melanoma have not been fully elucidated. In the present study, a circRNA microarray was performed to analyze the variability of circRNAs in the low-metastatic melanoma WM35 cell line and in the high-metastatic melanoma WM451 cell line in comparison to control human melanocytes. The results revealed that five circRNAs were upregulated and four circRNAs were downregulated in both the WM35 and WM451 cells. qRT-PCR revealed an upregulated expression of circ0000082 and circ0016418 and a downregulation of circ0023988, circ0008157 and circ0030388 in the cells which was consistent with the results of the microarray assay. Functional tests revealed that knockdown of circ0023988, circ0008157 or circ0030388 significantly promoted the proliferation and invasion of the WM35 cells. Following the silencing of circ0000082 or circ0016418 in WM451 cells, the proliferation and invasion of the WM451 cells were inhibited. Bioinformatic analysis predicted that the circ0000082-, circ0023988-and circ0008157-circRNAmiRNA-mRNA network may participate in the occurrence, development, invasion and metastasis of malignant tumors. The present study revealed several differentially expressed circRNAs, indicating that the newly identified circRNAs may provide new therapeutic targets for melanoma.",
keywords = "CeRNA, Circular RNA, Melanoma, MiRNA, SiRNA",
author = "Qi Wang and Jia Chen and Aijun Wang and Lichun Sun and Li Qian and Xiao Zhou and Yu Liu and Shijie Tang and Xiang Chen and Yan Cheng and Ke Cao and Jianda Zhou",
year = "2018",
month = "4",
day = "1",
doi = "10.3892/or.2018.6263",
language = "English (US)",
volume = "39",
pages = "1813--1824",
journal = "Oncology Reports",
issn = "1021-335X",
publisher = "Spandidos Publications",
number = "4",

}

TY - JOUR

T1 - Differentially expressed circRNAs in melanocytes and melanoma cells and their effect on cell proliferation and invasion

AU - Wang, Qi

AU - Chen, Jia

AU - Wang, Aijun

AU - Sun, Lichun

AU - Qian, Li

AU - Zhou, Xiao

AU - Liu, Yu

AU - Tang, Shijie

AU - Chen, Xiang

AU - Cheng, Yan

AU - Cao, Ke

AU - Zhou, Jianda

PY - 2018/4/1

Y1 - 2018/4/1

N2 - Circular RNAs (circRNAs) play critical roles in the occurrence of human diseases, including cancer. However, the detailed functions of circRNAs in melanoma have not been fully elucidated. In the present study, a circRNA microarray was performed to analyze the variability of circRNAs in the low-metastatic melanoma WM35 cell line and in the high-metastatic melanoma WM451 cell line in comparison to control human melanocytes. The results revealed that five circRNAs were upregulated and four circRNAs were downregulated in both the WM35 and WM451 cells. qRT-PCR revealed an upregulated expression of circ0000082 and circ0016418 and a downregulation of circ0023988, circ0008157 and circ0030388 in the cells which was consistent with the results of the microarray assay. Functional tests revealed that knockdown of circ0023988, circ0008157 or circ0030388 significantly promoted the proliferation and invasion of the WM35 cells. Following the silencing of circ0000082 or circ0016418 in WM451 cells, the proliferation and invasion of the WM451 cells were inhibited. Bioinformatic analysis predicted that the circ0000082-, circ0023988-and circ0008157-circRNAmiRNA-mRNA network may participate in the occurrence, development, invasion and metastasis of malignant tumors. The present study revealed several differentially expressed circRNAs, indicating that the newly identified circRNAs may provide new therapeutic targets for melanoma.

AB - Circular RNAs (circRNAs) play critical roles in the occurrence of human diseases, including cancer. However, the detailed functions of circRNAs in melanoma have not been fully elucidated. In the present study, a circRNA microarray was performed to analyze the variability of circRNAs in the low-metastatic melanoma WM35 cell line and in the high-metastatic melanoma WM451 cell line in comparison to control human melanocytes. The results revealed that five circRNAs were upregulated and four circRNAs were downregulated in both the WM35 and WM451 cells. qRT-PCR revealed an upregulated expression of circ0000082 and circ0016418 and a downregulation of circ0023988, circ0008157 and circ0030388 in the cells which was consistent with the results of the microarray assay. Functional tests revealed that knockdown of circ0023988, circ0008157 or circ0030388 significantly promoted the proliferation and invasion of the WM35 cells. Following the silencing of circ0000082 or circ0016418 in WM451 cells, the proliferation and invasion of the WM451 cells were inhibited. Bioinformatic analysis predicted that the circ0000082-, circ0023988-and circ0008157-circRNAmiRNA-mRNA network may participate in the occurrence, development, invasion and metastasis of malignant tumors. The present study revealed several differentially expressed circRNAs, indicating that the newly identified circRNAs may provide new therapeutic targets for melanoma.

KW - CeRNA

KW - Circular RNA

KW - Melanoma

KW - MiRNA

KW - SiRNA

UR - http://www.scopus.com/inward/record.url?scp=85042774580&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85042774580&partnerID=8YFLogxK

U2 - 10.3892/or.2018.6263

DO - 10.3892/or.2018.6263

M3 - Article

C2 - 29436673

AN - SCOPUS:85042774580

VL - 39

SP - 1813

EP - 1824

JO - Oncology Reports

JF - Oncology Reports

SN - 1021-335X

IS - 4

ER -