Differential transport requirements of HLA and H-2 class I glycoproteins

Jeff Alexander, John A Payne, Richard Murray, Jeffrey A. Frelinger, Peter Cresswell

Research output: Contribution to journalArticle

130 Scopus citations

Abstract

Transport of human and mouse major histocompatibility complex class I glycoproteins has been examined in a transport deficient B-lymphoblastoid cell line × T-lymphoblastoid cell line (B-LCL × T-LCL) hybrid, 174 × CEM. T2 (T2). This cell line expresses no detectable endogenous HLA-B5 and reduced levels of HLA-A2 on its surface although these molecules are synthesized. In order to study this defect further, either HLA-Bw58 or HLA-B7 genomic clones were transfected into T2. Metabolic labeling and immune precipitation demonstrated biosynthesis of the Bw58 or 137 glycoprotein. However, like the endogenous HLA-B5 molecule, neither HLA-Bw58 nor HLA-B7 was expressed at the cell surface. The cloned genes were properly expressed on the surface of C1R, a control B-LCL. To determine if mouse class I alleles had the same transport requirements as the human class I glycoproteins, either mouse H-2Dpor H-2Kb class I genes were introduced into T2. Surprisingly, the H-2 class I glycoproteins were transported to the cell surface normally. These data suggest a fundamental difference between human and mouse histocompatibility antigens in their requirements for intracellular transport.

Original languageEnglish (US)
Pages (from-to)380-388
Number of pages9
JournalImmunogenetics
Volume29
Issue number6
DOIs
StatePublished - Jun 1989
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Immunology
  • Genetics

Cite this