Differential subcellular regulation of NMDAR1 protein and mRNA in dendrites of dentate gyrus granule cells after perforant path transection

Adam H. Gazzaley, Deanna L. Benson, George W. Huntley, John Morrison

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

Unilateral transection of the excitatory perforant path results in the acute deafferentation of a segregated zone on the distal dendrites of hippocampal dentate gyrus granule cells (i.e., outer molecular layer), followed by sprouting, reactive synaptogenesis, and a return of physiological and behavioral function. To investigate cellular mechanisms underlying NMDA receptor plasticity in response to such extensive synaptic reorganization, we quantitatively evaluated changes in intensity levels of NMDAR1 immunofluorescence and NMDAR1 mRNA hybridization within subcellular compartments of dentate gyrus granule cells 2, 5, and 9 d after perforant path lesions. There were no significant changes in either measure at 2 d postlesion. However, at 5 and 9 d postlesion, during the period of axonal sprouting and synaptogenesis, there was an increase in NMDAR1 immunolabeling that was restricted to the dendritic segments of the denervated outer molecular layer and the granule cell somata. In contrast, NMDAR1 mRNA levels at 5 and 9 d postlesion increased throughout the full extent of the molecular layer, including both denervated and nondenervated segments of granule cell dendrites. These findings reveal that NMDAR1 mRNA is one of a limited population of mRNAs that is transported into dendrites and further suggest that in response to terminal proliferation and sprouting, increased mRNA transport occurs throughout the full dendritic extent, whereas increased local protein synthesis is restricted to denervated regions of the dendrites whose afferent activity is perturbed. These results begin to elucidate the dynamic postsynaptic subcellular regulation of receptor subunits associated with synaptic plasticity after denervation.

Original languageEnglish (US)
Pages (from-to)2006-2017
Number of pages12
JournalJournal of Neuroscience
Volume17
Issue number6
StatePublished - Mar 15 1997
Externally publishedYes

Fingerprint

Perforant Pathway
Dentate Gyrus
Dendrites
Messenger RNA
Proteins
Parahippocampal Gyrus
Neuronal Plasticity
Carisoprodol
Denervation
N-Methyl-D-Aspartate Receptors
Fluorescent Antibody Technique
NMDA receptor A1
Population

Keywords

  • confocal microscopy
  • entorhinal cortex
  • excitatory amino acid receptors
  • hippocampus
  • immunocytochemistry
  • plasticity

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Differential subcellular regulation of NMDAR1 protein and mRNA in dendrites of dentate gyrus granule cells after perforant path transection. / Gazzaley, Adam H.; Benson, Deanna L.; Huntley, George W.; Morrison, John.

In: Journal of Neuroscience, Vol. 17, No. 6, 15.03.1997, p. 2006-2017.

Research output: Contribution to journalArticle

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