Abstract
Endogenous, constitutive soluble epoxide hydrolase in mice 3T3 cells was localized via immunofluorescence microscopy exclusively in peroxisomes, whereas transiently expressed mouse soluble epoxide hydrolase (from clofibrate-treated liver) accumulated only in the cytosol of 3T3 and HeLa cells. When the C-terminal Ile of mouse soluble epoxide hydrolase was mutated to generate a prototypic putative type 1 PTS (-SKI to -SKL), the enzyme targeted to peroxisomes. The possibility that soluble epoxide hydrolase-SKI was sorted slowly to peroxiosmes from the cytosol was examined by stably expressing rat soluble epoxide hydrolase-SKI appended to the green fluorescent protein. Green fluorescent protein soluble epoxide hydrolase-SKI was strictly cytosolic, indicating that -SKI was not a temporally inefficient putative type 1 PTS. Import of soluble epoxide hydrolase-SKI into peroxisomes in plant cells revealed that the context of -SKI on soluble epoxide hydrolase was targeting permissible. These results show that the C-terminal -SKI is a non-functional putative type 1 PTS on soluble epoxide hydrolase and suggest the existence of distinct cytosolic and peroxisomal targeting variants of soluble epoxide hydrolase in mouse and rat. Copyright (C) 1999 Federation of European Biochemical Societies.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 301-305 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 445 |
| Issue number | 2-3 |
| DOIs | |
| State | Published - Feb 26 1999 |
Fingerprint
Keywords
- Clofibrate
- Epoxide hydrolase
- Peroxisome
- Peroxisome targeting signal
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Differential subcellular localization of endogenous and transfected soluble epoxide hydrolase in mammalian cells : Evidence for isozyme variants. / Mullen, Robert T.; Trelease, Richard N.; Duerk, Heike; Arand, Michael; Hammock, Bruce D.; Oesch, Franz; Grant, David F.
In: FEBS Letters, Vol. 445, No. 2-3, 26.02.1999, p. 301-305.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Differential subcellular localization of endogenous and transfected soluble epoxide hydrolase in mammalian cells
T2 - Evidence for isozyme variants
AU - Mullen, Robert T.
AU - Trelease, Richard N.
AU - Duerk, Heike
AU - Arand, Michael
AU - Hammock, Bruce D.
AU - Oesch, Franz
AU - Grant, David F.
PY - 1999/2/26
Y1 - 1999/2/26
N2 - Endogenous, constitutive soluble epoxide hydrolase in mice 3T3 cells was localized via immunofluorescence microscopy exclusively in peroxisomes, whereas transiently expressed mouse soluble epoxide hydrolase (from clofibrate-treated liver) accumulated only in the cytosol of 3T3 and HeLa cells. When the C-terminal Ile of mouse soluble epoxide hydrolase was mutated to generate a prototypic putative type 1 PTS (-SKI to -SKL), the enzyme targeted to peroxisomes. The possibility that soluble epoxide hydrolase-SKI was sorted slowly to peroxiosmes from the cytosol was examined by stably expressing rat soluble epoxide hydrolase-SKI appended to the green fluorescent protein. Green fluorescent protein soluble epoxide hydrolase-SKI was strictly cytosolic, indicating that -SKI was not a temporally inefficient putative type 1 PTS. Import of soluble epoxide hydrolase-SKI into peroxisomes in plant cells revealed that the context of -SKI on soluble epoxide hydrolase was targeting permissible. These results show that the C-terminal -SKI is a non-functional putative type 1 PTS on soluble epoxide hydrolase and suggest the existence of distinct cytosolic and peroxisomal targeting variants of soluble epoxide hydrolase in mouse and rat. Copyright (C) 1999 Federation of European Biochemical Societies.
AB - Endogenous, constitutive soluble epoxide hydrolase in mice 3T3 cells was localized via immunofluorescence microscopy exclusively in peroxisomes, whereas transiently expressed mouse soluble epoxide hydrolase (from clofibrate-treated liver) accumulated only in the cytosol of 3T3 and HeLa cells. When the C-terminal Ile of mouse soluble epoxide hydrolase was mutated to generate a prototypic putative type 1 PTS (-SKI to -SKL), the enzyme targeted to peroxisomes. The possibility that soluble epoxide hydrolase-SKI was sorted slowly to peroxiosmes from the cytosol was examined by stably expressing rat soluble epoxide hydrolase-SKI appended to the green fluorescent protein. Green fluorescent protein soluble epoxide hydrolase-SKI was strictly cytosolic, indicating that -SKI was not a temporally inefficient putative type 1 PTS. Import of soluble epoxide hydrolase-SKI into peroxisomes in plant cells revealed that the context of -SKI on soluble epoxide hydrolase was targeting permissible. These results show that the C-terminal -SKI is a non-functional putative type 1 PTS on soluble epoxide hydrolase and suggest the existence of distinct cytosolic and peroxisomal targeting variants of soluble epoxide hydrolase in mouse and rat. Copyright (C) 1999 Federation of European Biochemical Societies.
KW - Clofibrate
KW - Epoxide hydrolase
KW - Peroxisome
KW - Peroxisome targeting signal
UR - http://www.scopus.com/inward/record.url?scp=0032979423&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032979423&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(99)00142-8
DO - 10.1016/S0014-5793(99)00142-8
M3 - Article
C2 - 10094477
AN - SCOPUS:0032979423
VL - 445
SP - 301
EP - 305
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 2-3
ER -