Abstract
The diagnostic sensitivities of the polymerase chain reaction (PCR) and culture were compared and correlated with clinical signs in 5 vaccinated cats and 3 unvaccinated cats that were experimentally infected with feline herpesvirus 1. Conjunctival swabs were taken each day from 0 to 14 days and on 21,28 and 30 days after challenge. PCR (49.3%) was significantly more sensitive than culture (30.1%) as assessed by an adjusted McNemar's test to account for non-independence of results between days within each cat (P = 0.02). PCR was considerably more sensitive (34.1%) than culture (8.2%) in vaccinated cats (P=0.001), whereas there was no significant difference ill sensitivities in the unvaccinated cats, where the sensitivity of PCR was 74.5% and that of culture was 66.7% (P=0.17). In vaccinated cats showing clinical signs, the sensitivities of culture and PCR were 14.8% and 55.6% respectively(P = 0.03), whereas in unvaccinated cats the sensitivities were 80.6% and 96.8% respectively (P=0.07). This study suggests that disease due to feline herpesvirus 1 has been significantly underdiagnosed, particularly in vaccinated cats.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 65-74 |
| Number of pages | 10 |
| Journal | Archives of Virology |
| Volume | 142 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1997 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Genetics
- Applied Microbiology and Biotechnology
Cite this
Differential sensitivity of culture and the polymerase chain reaction for detection of feline herpesvirus 1 in vaccinated and unvaccinated cats. / Sykes, Jane E; Browning, G. F.; Anderson, G.; Studdert, V. P.; Smith, H. V.
In: Archives of Virology, Vol. 142, No. 1, 1997, p. 65-74.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Differential sensitivity of culture and the polymerase chain reaction for detection of feline herpesvirus 1 in vaccinated and unvaccinated cats
AU - Sykes, Jane E
AU - Browning, G. F.
AU - Anderson, G.
AU - Studdert, V. P.
AU - Smith, H. V.
PY - 1997
Y1 - 1997
N2 - The diagnostic sensitivities of the polymerase chain reaction (PCR) and culture were compared and correlated with clinical signs in 5 vaccinated cats and 3 unvaccinated cats that were experimentally infected with feline herpesvirus 1. Conjunctival swabs were taken each day from 0 to 14 days and on 21,28 and 30 days after challenge. PCR (49.3%) was significantly more sensitive than culture (30.1%) as assessed by an adjusted McNemar's test to account for non-independence of results between days within each cat (P = 0.02). PCR was considerably more sensitive (34.1%) than culture (8.2%) in vaccinated cats (P=0.001), whereas there was no significant difference ill sensitivities in the unvaccinated cats, where the sensitivity of PCR was 74.5% and that of culture was 66.7% (P=0.17). In vaccinated cats showing clinical signs, the sensitivities of culture and PCR were 14.8% and 55.6% respectively(P = 0.03), whereas in unvaccinated cats the sensitivities were 80.6% and 96.8% respectively (P=0.07). This study suggests that disease due to feline herpesvirus 1 has been significantly underdiagnosed, particularly in vaccinated cats.
AB - The diagnostic sensitivities of the polymerase chain reaction (PCR) and culture were compared and correlated with clinical signs in 5 vaccinated cats and 3 unvaccinated cats that were experimentally infected with feline herpesvirus 1. Conjunctival swabs were taken each day from 0 to 14 days and on 21,28 and 30 days after challenge. PCR (49.3%) was significantly more sensitive than culture (30.1%) as assessed by an adjusted McNemar's test to account for non-independence of results between days within each cat (P = 0.02). PCR was considerably more sensitive (34.1%) than culture (8.2%) in vaccinated cats (P=0.001), whereas there was no significant difference ill sensitivities in the unvaccinated cats, where the sensitivity of PCR was 74.5% and that of culture was 66.7% (P=0.17). In vaccinated cats showing clinical signs, the sensitivities of culture and PCR were 14.8% and 55.6% respectively(P = 0.03), whereas in unvaccinated cats the sensitivities were 80.6% and 96.8% respectively (P=0.07). This study suggests that disease due to feline herpesvirus 1 has been significantly underdiagnosed, particularly in vaccinated cats.
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UR - http://www.scopus.com/inward/citedby.url?scp=0031023106&partnerID=8YFLogxK
U2 - 10.1007/s007050050059
DO - 10.1007/s007050050059
M3 - Article
C2 - 9155873
AN - SCOPUS:0031023106
VL - 142
SP - 65
EP - 74
JO - Archives of Virology
JF - Archives of Virology
SN - 0304-8608
IS - 1
ER -