Differential response of bovine T-cell lines to membrane and soluble antigens of Theileria parva schizont-infected cells

W. C. Brown, C. Sugimoto, Patricia A Conrad, D. J. Grab

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

T-cell lines generated from Theileria parva-immune cattle were used to identify antigens associated with schizont-infected lymphoblastoid cells. Homogenates prepared from T. parva-infected cells were fractionated by differential centrifugation, and antigenically distinct soluble and membrane-bound antigens were detected by the differential stimulation of cell lines derived from two animals. Activity in the soluble fraction was not attributable to either a mitogen or interleukin 2. Activity in the membrane fraction was associated with schizont membranes as indicated by the presence in this fraction of a parasite protein detected by immunoblot analysis using a schizont-specific monoclonal antibody. Elimination of intracellular schizonts over time, using the anti-theilerial drug, parvaquone, resulted in a concomitant loss of antigenicity in infected cells and in subcellular fractions prepared from drug-treated cells, demonstrating that stimulation of Theileria-specific helper and cytotoxic T-cell responses is associated with the presence of the parasite.

Original languageEnglish (US)
Pages (from-to)567-583
Number of pages17
JournalParasite Immunology
Volume11
Issue number6
StatePublished - 1989
Externally publishedYes

Keywords

  • antigens
  • cattle
  • schizont
  • T-lymphocytes
  • Theileria parva

ASJC Scopus subject areas

  • Parasitology
  • Immunology

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