Differential regulation of NMDAR1 mRNA and protein by estradiol in the rat hippocampus

Adam H. Gazzaley, Nancy G. Weiland, Bruce S. McEwen, John Morrison

Research output: Contribution to journalArticle

336 Citations (Scopus)

Abstract

Estradiol treatment increases the number of NMDA receptor binding sites, and changes evoked synaptic currents in a manner consistent with a steroid- induced functional enhancement of NMDA receptors in rat hippocampus. In this study, we investigate the cellular mechanisms of estradiol-induced NMDA receptor regulation at the protein and mRNA levels in ovariectomized rats treated with ovarian steroids using immunocytochemical and in situ hybridization techniques. Confocal laser scanning microscopy was used to quantify alterations in immunofluorescence intensity levels of NMDAR1 subunit proteins within neuronal somata and dendrites of discrete hippocampal fields, whereas in parallel, in situ hybridization was used to examine NMDAR1 mRNA levels in corresponding hippocampal regions. The data indicate that estradiol treatment in ovariectomized rats significantly increases immunofluorescence intensity levels in comparison with nonsteroid treated ovariectomized rats within the somata and dendrites of CA1 pyramidal cells and, to a lesser extent, within the granule cell somata of the dentate gyrus. In contrast, such alterations in immunofluorescence intensity occur without concomitant changes in mRNA hybridization levels. Thus, these data suggest that estradiol modulates NMDA receptor function via post-transcriptional regulation of the NMDAR1 subunit protein. The increase in immunofluorescence intensity may reflect an increase in the concentration of the subunit protein, which could account for estrogen-induced changes in pharmacological and physiological properties of the NMDA receptor.

Original languageEnglish (US)
Pages (from-to)6830-6838
Number of pages9
JournalJournal of Neuroscience
Volume16
Issue number21
StatePublished - Nov 1 1996
Externally publishedYes

Fingerprint

N-Methyl-D-Aspartate Receptors
Estradiol
Hippocampus
Fluorescent Antibody Technique
Protein Subunits
Carisoprodol
Messenger RNA
Dendrites
Proteins
In Situ Hybridization
Steroids
Pyramidal Cells
Dentate Gyrus
Confocal Microscopy
Estrogens
Binding Sites
NMDA receptor A1
Pharmacology
Therapeutics

Keywords

  • CA1
  • confocal microscopy
  • estrogen
  • excitatory amino acid receptors
  • immunocytochemistry
  • in situ hybridization
  • NMDAR1

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Differential regulation of NMDAR1 mRNA and protein by estradiol in the rat hippocampus. / Gazzaley, Adam H.; Weiland, Nancy G.; McEwen, Bruce S.; Morrison, John.

In: Journal of Neuroscience, Vol. 16, No. 21, 01.11.1996, p. 6830-6838.

Research output: Contribution to journalArticle

Gazzaley, Adam H. ; Weiland, Nancy G. ; McEwen, Bruce S. ; Morrison, John. / Differential regulation of NMDAR1 mRNA and protein by estradiol in the rat hippocampus. In: Journal of Neuroscience. 1996 ; Vol. 16, No. 21. pp. 6830-6838.
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