Differential localization of the high- and low-molecular weight variants of MAP2 in the developing retina

Richard P Tucker, Lester I. Binder, Andrew I. Matus

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Microtubule-associated protein 2 (MAP2) occurs in developing mammalian neuronal tissue as both high (280 kDa)- and low (70 kDa)-molecular weight forms with temporally regulated expression. We have studied the developing avian retina with a monoclonal antibody that recognizes both the high- and low-molecular weight forms of MAP2 and a second monoclonal antibody that recognizes only high-molecular weight MAP2. The developmentally regulated, low-molecular weight protein, MAP2c, has a more widespread distribution in the embryonic avian retina than high-molecular weight MAP2. Our results suggest that MAP2c is the first form of MAP2 to appear in differentiated embryonic retinal neurons, and that the high-molecular weight isoforms of MAP2 appear only later when they may confer stability to neuronal processes.

Original languageEnglish (US)
Pages (from-to)313-318
Number of pages6
JournalDevelopmental Brain Research
Volume38
Issue number2
DOIs
StatePublished - Feb 1 1988
Externally publishedYes

Fingerprint

Microtubule-Associated Proteins
Retina
Molecular Weight
Monoclonal Antibodies
Retinal Neurons
Protein Isoforms
Proteins

Keywords

  • Development
  • Immunohistochemistry
  • MAP2
  • MAP2c
  • Microtubule-associated protein
  • Neuronal differentiation
  • Retina

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

Differential localization of the high- and low-molecular weight variants of MAP2 in the developing retina. / Tucker, Richard P; Binder, Lester I.; Matus, Andrew I.

In: Developmental Brain Research, Vol. 38, No. 2, 01.02.1988, p. 313-318.

Research output: Contribution to journalArticle

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