Differential induction of tumor necrosis factor alpha in ovine pulmonary alveolar macrophages following infection with Corynebacterium pseudotuberculosis, Pasteurella haemolytica, or lentiviruses

J. A. Ellis, Michael Dale Lairmore, D. T. O'Toole, M. Campos

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24 Citations (Scopus)

Abstract

Soluble mediators such as tumor necrosis factor alpha (TNF-α) may be important in the pathogenesis of many chronic pulmonary infections. We examined the ability of Corynebacterium pseudotuberculosis, Pasteurella haemolytica, and ovine lentiviruses (OvLV) to induce TNF-α secretion by pulmonary alveolar macrophages (PAM). Bronchoalveolar lavage cells, composed of greater than 90% PAM, were obtained from normal sheep. Bronchoalveolar lavage cells were cultured for 2, 24, 48, 72, or 168 h in endotoxin-free RPMI medium (with 10% autologous serum) or in medium containing one of the following additives: lipopolysaccharide, 1-μm polystyrene beads, C. pseudotuberculosis, P. haemolytica, or one of two plaque-cloned OvLV, 85/28 or 85/34. Lipopolysaccharide, C. pseudotuberculosis, and P. haemolytica induced TNF-α activity in PAM cultures as early as 2 h after inoculation, as assessed by a colorimetric cytotoxicity assay. This activity could be blocked by rabbit anti-recombinant bovine TNF-α serum. In contrast, medium alone, polystyrene beads, and productive infection by OvLV did not induce TNF-α activity in PAM cultures. Bacterial pathogens which infect pulmonary macrophages may elicit the secretion of TNF-α within the lungs and lead to the cachectic state associated with chronic pneumonia.

Original languageEnglish (US)
Pages (from-to)3254-3260
Number of pages7
JournalInfection and Immunity
Volume59
Issue number9
StatePublished - 1991
Externally publishedYes

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Corynebacterium pseudotuberculosis
Mannheimia haemolytica
Lentivirus
Alveolar Macrophages
Sheep
Tumor Necrosis Factor-alpha
Infection
Polystyrenes
Bronchoalveolar Lavage
Lipopolysaccharides
Lentivirus Infections
Lung
Serum
Endotoxins
Cultured Cells
Pneumonia

ASJC Scopus subject areas

  • Immunology

Cite this

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title = "Differential induction of tumor necrosis factor alpha in ovine pulmonary alveolar macrophages following infection with Corynebacterium pseudotuberculosis, Pasteurella haemolytica, or lentiviruses",
abstract = "Soluble mediators such as tumor necrosis factor alpha (TNF-α) may be important in the pathogenesis of many chronic pulmonary infections. We examined the ability of Corynebacterium pseudotuberculosis, Pasteurella haemolytica, and ovine lentiviruses (OvLV) to induce TNF-α secretion by pulmonary alveolar macrophages (PAM). Bronchoalveolar lavage cells, composed of greater than 90{\%} PAM, were obtained from normal sheep. Bronchoalveolar lavage cells were cultured for 2, 24, 48, 72, or 168 h in endotoxin-free RPMI medium (with 10{\%} autologous serum) or in medium containing one of the following additives: lipopolysaccharide, 1-μm polystyrene beads, C. pseudotuberculosis, P. haemolytica, or one of two plaque-cloned OvLV, 85/28 or 85/34. Lipopolysaccharide, C. pseudotuberculosis, and P. haemolytica induced TNF-α activity in PAM cultures as early as 2 h after inoculation, as assessed by a colorimetric cytotoxicity assay. This activity could be blocked by rabbit anti-recombinant bovine TNF-α serum. In contrast, medium alone, polystyrene beads, and productive infection by OvLV did not induce TNF-α activity in PAM cultures. Bacterial pathogens which infect pulmonary macrophages may elicit the secretion of TNF-α within the lungs and lead to the cachectic state associated with chronic pneumonia.",
author = "Ellis, {J. A.} and Lairmore, {Michael Dale} and O'Toole, {D. T.} and M. Campos",
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T1 - Differential induction of tumor necrosis factor alpha in ovine pulmonary alveolar macrophages following infection with Corynebacterium pseudotuberculosis, Pasteurella haemolytica, or lentiviruses

AU - Ellis, J. A.

AU - Lairmore, Michael Dale

AU - O'Toole, D. T.

AU - Campos, M.

PY - 1991

Y1 - 1991

N2 - Soluble mediators such as tumor necrosis factor alpha (TNF-α) may be important in the pathogenesis of many chronic pulmonary infections. We examined the ability of Corynebacterium pseudotuberculosis, Pasteurella haemolytica, and ovine lentiviruses (OvLV) to induce TNF-α secretion by pulmonary alveolar macrophages (PAM). Bronchoalveolar lavage cells, composed of greater than 90% PAM, were obtained from normal sheep. Bronchoalveolar lavage cells were cultured for 2, 24, 48, 72, or 168 h in endotoxin-free RPMI medium (with 10% autologous serum) or in medium containing one of the following additives: lipopolysaccharide, 1-μm polystyrene beads, C. pseudotuberculosis, P. haemolytica, or one of two plaque-cloned OvLV, 85/28 or 85/34. Lipopolysaccharide, C. pseudotuberculosis, and P. haemolytica induced TNF-α activity in PAM cultures as early as 2 h after inoculation, as assessed by a colorimetric cytotoxicity assay. This activity could be blocked by rabbit anti-recombinant bovine TNF-α serum. In contrast, medium alone, polystyrene beads, and productive infection by OvLV did not induce TNF-α activity in PAM cultures. Bacterial pathogens which infect pulmonary macrophages may elicit the secretion of TNF-α within the lungs and lead to the cachectic state associated with chronic pneumonia.

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