Differential induction of tumor necrosis factor alpha in ovine pulmonary alveolar macrophages following infection with Corynebacterium pseudotuberculosis, Pasteurella haemolytica, or lentiviruses

J. A. Ellis, Michael Dale Lairmore, D. T. O'Toole, M. Campos

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24 Scopus citations


Soluble mediators such as tumor necrosis factor alpha (TNF-α) may be important in the pathogenesis of many chronic pulmonary infections. We examined the ability of Corynebacterium pseudotuberculosis, Pasteurella haemolytica, and ovine lentiviruses (OvLV) to induce TNF-α secretion by pulmonary alveolar macrophages (PAM). Bronchoalveolar lavage cells, composed of greater than 90% PAM, were obtained from normal sheep. Bronchoalveolar lavage cells were cultured for 2, 24, 48, 72, or 168 h in endotoxin-free RPMI medium (with 10% autologous serum) or in medium containing one of the following additives: lipopolysaccharide, 1-μm polystyrene beads, C. pseudotuberculosis, P. haemolytica, or one of two plaque-cloned OvLV, 85/28 or 85/34. Lipopolysaccharide, C. pseudotuberculosis, and P. haemolytica induced TNF-α activity in PAM cultures as early as 2 h after inoculation, as assessed by a colorimetric cytotoxicity assay. This activity could be blocked by rabbit anti-recombinant bovine TNF-α serum. In contrast, medium alone, polystyrene beads, and productive infection by OvLV did not induce TNF-α activity in PAM cultures. Bacterial pathogens which infect pulmonary macrophages may elicit the secretion of TNF-α within the lungs and lead to the cachectic state associated with chronic pneumonia.

Original languageEnglish (US)
Pages (from-to)3254-3260
Number of pages7
JournalInfection and Immunity
Issue number9
StatePublished - 1991
Externally publishedYes


ASJC Scopus subject areas

  • Immunology

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