Differential gene expression induced by exposure of captive mink to fuel oil

A model for the sea otter

Lizabeth Bowen, Federica Riva, Frederick C Mohr, Brian Aldridge, Julie Schwartz, A. Keith Miles, Jeffrey L Stott

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Free-ranging sea otters are subject to hydrocarbon exposure from a variety of sources, both natural and anthropogenic. Effects of direct exposure to unrefined crude oil, such as that associated with the Exxon Valdez oil spill, are readily apparent. However, the impact of subtle but pathophysiologically relevant concentrations of crude oil on sea otters is difficult to assess. The present study was directed at developing a model for assessing the impact of low concentrations of fuel oil on sea otters. Quantitative PCR was used to identify differential gene expression in American mink that were exposed to low concentrations of bunker C fuel oil. A total of 23 genes, representing 10 different physiological systems, were analyzed for perturbation. Six genes with immunological relevance were differentially expressed in oil-fed mink. Interleukin-18 (IL-18), IL-10, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), and complement cytolysis inhibitor (CLI) were down-regulated while IL-2 was up-regulated. Expression of two additional genes was affected; heat shock protein 70 (HSP70) was up-regulated and thyroid hormone receptor (THR) was down-regulated. While the significance of each perturbation is not immediately evident, we identified differential expression of genes that would be consistent with the presence of immune system-modifying and endocrine-disrupting compounds in fuel oil. Application of this approach to identify effects of petroleum contamination on sea otters should be possible following expansion of this mink model to identify a greater number of affected genes in peripheral blood leukocytes.

Original languageEnglish (US)
Pages (from-to)298-309
Number of pages12
JournalEcoHealth
Volume4
Issue number3
DOIs
StatePublished - Sep 2007

Fingerprint

Otters
Fuel Oils
Mink
gene expression
Petroleum
Gene Expression
gene
Genes
Petroleum Pollution
crude oil
Thyroid Hormone Receptors
Interleukin-18
HSP70 Heat-Shock Proteins
perturbation
Nitric Oxide Synthase Type II
Cyclooxygenase 2
Hydrocarbons
Interleukin-10
Interleukin-2
heat shock

Keywords

  • Differential gene expression
  • Mink
  • Petroleum
  • Sea otter

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Nature and Landscape Conservation

Cite this

Differential gene expression induced by exposure of captive mink to fuel oil : A model for the sea otter. / Bowen, Lizabeth; Riva, Federica; Mohr, Frederick C; Aldridge, Brian; Schwartz, Julie; Miles, A. Keith; Stott, Jeffrey L.

In: EcoHealth, Vol. 4, No. 3, 09.2007, p. 298-309.

Research output: Contribution to journalArticle

Bowen, Lizabeth ; Riva, Federica ; Mohr, Frederick C ; Aldridge, Brian ; Schwartz, Julie ; Miles, A. Keith ; Stott, Jeffrey L. / Differential gene expression induced by exposure of captive mink to fuel oil : A model for the sea otter. In: EcoHealth. 2007 ; Vol. 4, No. 3. pp. 298-309.
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