Abstract
Retinoic acid, mediated through its receptors (RAR), exhibits striking effects on cell proliferation, differentiation, and pattern formation. At least three types of RARs (α, β, and γ) have been cloned. The ontogeny and semi-quantitated amount of three RAR mRNA were studied in mouse embryos and placentas using reverse transcriptase-polymerase chain reaction technique. The amplified cDNA products were confirmed by correct sizes, Southern blot hybridization and restriction enzyme digestion. The results show that RAR-α mRNA is constitutively expressed in the oocytes and embryos of all stages, suggesting that RAR-α is a housekeeping gene. RAR-β mRNA is present in the oocytes, becomes undetectable at the 2-cell stage when the maternal RNA is degraded, and re-expresses at morula and blastocst stages when the embryonic genome is activated. RAR-γ mRNA is not expressed until blastocyst stage. In the postimplantation embryos, no significant change in all three RAR mRNA levels was detected from day 10 through the late gestational stage (day 18). Placental tissue expressed all three RAR transcripts throughout gestation. The differential activation of these three RAR genes in the preimplantation embryos suggests RARs as the important regulators during early embryogenesis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 685-691 |
| Number of pages | 7 |
| Journal | Development Growth and Differentiation |
| Volume | 34 |
| Issue number | 6 |
| DOIs | |
| State | Published - 1992 |
Fingerprint
ASJC Scopus subject areas
- Anatomy
- Developmental Biology
- Cell Biology
Cite this
Differential expression of retinoic acid receptor mRNA during mouse embryogenesis. / Wu, T. C J; Wang, L.; Wan, Yu-Jui Yvonne.
In: Development Growth and Differentiation, Vol. 34, No. 6, 1992, p. 685-691.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Differential expression of retinoic acid receptor mRNA during mouse embryogenesis
AU - Wu, T. C J
AU - Wang, L.
AU - Wan, Yu-Jui Yvonne
PY - 1992
Y1 - 1992
N2 - Retinoic acid, mediated through its receptors (RAR), exhibits striking effects on cell proliferation, differentiation, and pattern formation. At least three types of RARs (α, β, and γ) have been cloned. The ontogeny and semi-quantitated amount of three RAR mRNA were studied in mouse embryos and placentas using reverse transcriptase-polymerase chain reaction technique. The amplified cDNA products were confirmed by correct sizes, Southern blot hybridization and restriction enzyme digestion. The results show that RAR-α mRNA is constitutively expressed in the oocytes and embryos of all stages, suggesting that RAR-α is a housekeeping gene. RAR-β mRNA is present in the oocytes, becomes undetectable at the 2-cell stage when the maternal RNA is degraded, and re-expresses at morula and blastocst stages when the embryonic genome is activated. RAR-γ mRNA is not expressed until blastocyst stage. In the postimplantation embryos, no significant change in all three RAR mRNA levels was detected from day 10 through the late gestational stage (day 18). Placental tissue expressed all three RAR transcripts throughout gestation. The differential activation of these three RAR genes in the preimplantation embryos suggests RARs as the important regulators during early embryogenesis.
AB - Retinoic acid, mediated through its receptors (RAR), exhibits striking effects on cell proliferation, differentiation, and pattern formation. At least three types of RARs (α, β, and γ) have been cloned. The ontogeny and semi-quantitated amount of three RAR mRNA were studied in mouse embryos and placentas using reverse transcriptase-polymerase chain reaction technique. The amplified cDNA products were confirmed by correct sizes, Southern blot hybridization and restriction enzyme digestion. The results show that RAR-α mRNA is constitutively expressed in the oocytes and embryos of all stages, suggesting that RAR-α is a housekeeping gene. RAR-β mRNA is present in the oocytes, becomes undetectable at the 2-cell stage when the maternal RNA is degraded, and re-expresses at morula and blastocst stages when the embryonic genome is activated. RAR-γ mRNA is not expressed until blastocyst stage. In the postimplantation embryos, no significant change in all three RAR mRNA levels was detected from day 10 through the late gestational stage (day 18). Placental tissue expressed all three RAR transcripts throughout gestation. The differential activation of these three RAR genes in the preimplantation embryos suggests RARs as the important regulators during early embryogenesis.
UR - http://www.scopus.com/inward/record.url?scp=0027082870&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027082870&partnerID=8YFLogxK
U2 - 10.1111/j.1440-169X.1992.tb00037.x
DO - 10.1111/j.1440-169X.1992.tb00037.x
M3 - Article
AN - SCOPUS:0027082870
VL - 34
SP - 685
EP - 691
JO - Development Growth and Differentiation
JF - Development Growth and Differentiation
SN - 0012-1592
IS - 6
ER -