Differential expression of ganglioside G(D3) by human leukocytes and leukemia cells

B. Siddiqui, J. Buehler, M. W. DeGregorio, B. A. Macher

Research output: Contribution to journalArticle

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Abstract

Gangliosides from normal leukocytes and the cells of 25 patients with acute and chronic leukemia were tested for the presence of the disialoganglioside II3-α-N-acetylneuraminosyl-α2→8-N-acetylneuraminosyllactosylceramide (G(D3)). G(D3) was detected by immunostaining thin-layer chromatographs with an anti-G(D3) monoclonal antibody (AbR24). Among the myeloid cells tested, acute leukemia cells were positive for G(D3), whereas chronic leukemia cells and normal neutrophils did not have detectable G(D3). A range of G(D3) reactivity was apparent within the acute myeloid leukemia cells; gangliosides from pure myeloid leukemia cells stained more intensely than those from leukemia cells with monocytic characteristics. All lymphocytic leukemia cells (chronic and acute) contained G(D3), but this ganglioside could not be detected in extracts from normal lymphocytes. A ganglioside extract from the cells of a patient with hairy cell leukemia was also positive for G(D3) immunostaining. These results demonstrate that normal leukocytes and chronic myelogenous leukemia cells are distinguished from other lymphoid and nonlymphoid leukemia cells on the basis of G(D3) ganglioside expression.

Original languageEnglish (US)
Pages (from-to)5262-5265
Number of pages4
JournalCancer Research
Volume44
Issue number11
StatePublished - 1984
Externally publishedYes

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Leukemia
Leukocytes
Gangliosides
Myeloid Cells
Lymphoid Leukemia
Hairy Cell Leukemia
Myeloid Leukemia
B-Cell Chronic Lymphocytic Leukemia
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
GD3 ganglioside
Cell Extracts
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Acute Myeloid Leukemia
Neutrophils
Monoclonal Antibodies
Lymphocytes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Siddiqui, B., Buehler, J., DeGregorio, M. W., & Macher, B. A. (1984). Differential expression of ganglioside G(D3) by human leukocytes and leukemia cells. Cancer Research, 44(11), 5262-5265.

Differential expression of ganglioside G(D3) by human leukocytes and leukemia cells. / Siddiqui, B.; Buehler, J.; DeGregorio, M. W.; Macher, B. A.

In: Cancer Research, Vol. 44, No. 11, 1984, p. 5262-5265.

Research output: Contribution to journalArticle

Siddiqui, B, Buehler, J, DeGregorio, MW & Macher, BA 1984, 'Differential expression of ganglioside G(D3) by human leukocytes and leukemia cells', Cancer Research, vol. 44, no. 11, pp. 5262-5265.
Siddiqui B, Buehler J, DeGregorio MW, Macher BA. Differential expression of ganglioside G(D3) by human leukocytes and leukemia cells. Cancer Research. 1984;44(11):5262-5265.
Siddiqui, B. ; Buehler, J. ; DeGregorio, M. W. ; Macher, B. A. / Differential expression of ganglioside G(D3) by human leukocytes and leukemia cells. In: Cancer Research. 1984 ; Vol. 44, No. 11. pp. 5262-5265.
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