Differential expression of galectin-1 and galectin-3 during first trimester human embryogenesis

Frédéric A. Van Den Brûle, Pedro L. Fernandez, Crina Buicu, Fu-Tong Liu, Pascale Jackers, René Lambotte, Vincent Castronovo

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Development of complex organisms requires specific temporospatial differentiation and expression of the correct phenotype through activation of a variety of genes. Galectins are mammalian lectins able to interact with various extracellular matrix glycoconjugates and have been implicated in several biological events including cell attachment, differentiation, apoptosis, embryogenesis, and cancer invasion and metastasis. In this study, we have examined the expression of galectin-1 and galectin-3 during human first trimester embryogenesis using immunohistochemistry and Western blotting. Variable amounts of galectin-1 and galectin-3 were detected in all tissue protein extracts. Galectin-1 expression was demonstrated in the connective tissue and derived tissues such as smooth and striated muscle cells, and in some epithelia, such as in the basal layers of the skin after 14 weeks and in the epithelial cells of the gonads. Galectin-3 was detected mainly in epithelia, such as the skin, epithelial lining of the digestive and respiratory tract, and urothelium and excretory tubes of the kidney; but also in the myocardial cells, in the peripheral and preossifying hypertrophic chondrocytes, and in the notochord and in the liver. Our study constitutes the first demonstration of galectin-1 and galectin-3 during human embryogenesis. The differential expression of these two lectins suggests that they could participate in the complex processes of tissue differentiation.

Original languageEnglish (US)
Pages (from-to)399-405
Number of pages7
JournalDevelopmental Dynamics
Volume209
Issue number4
DOIs
StatePublished - 1997

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Galectin 1
Galectin 3
First Pregnancy Trimester
Embryonic Development
Lectins
Epithelium
Galectins
Notochord
Urothelium
Skin
Glycoconjugates
Tissue Extracts
Striated Muscle
Gonads
Chondrocytes
Respiratory System
Connective Tissue
Smooth Muscle Myocytes
Extracellular Matrix
Gastrointestinal Tract

Keywords

  • Embryogenesis
  • Galectin-1
  • Galectin-3
  • Human

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Van Den Brûle, F. A., Fernandez, P. L., Buicu, C., Liu, F-T., Jackers, P., Lambotte, R., & Castronovo, V. (1997). Differential expression of galectin-1 and galectin-3 during first trimester human embryogenesis. Developmental Dynamics, 209(4), 399-405. https://doi.org/10.1002/(SICI)1097-0177(199708)209:4<399::AID-AJA7>3.0.CO;2-D

Differential expression of galectin-1 and galectin-3 during first trimester human embryogenesis. / Van Den Brûle, Frédéric A.; Fernandez, Pedro L.; Buicu, Crina; Liu, Fu-Tong; Jackers, Pascale; Lambotte, René; Castronovo, Vincent.

In: Developmental Dynamics, Vol. 209, No. 4, 1997, p. 399-405.

Research output: Contribution to journalArticle

Van Den Brûle, FA, Fernandez, PL, Buicu, C, Liu, F-T, Jackers, P, Lambotte, R & Castronovo, V 1997, 'Differential expression of galectin-1 and galectin-3 during first trimester human embryogenesis', Developmental Dynamics, vol. 209, no. 4, pp. 399-405. https://doi.org/10.1002/(SICI)1097-0177(199708)209:4<399::AID-AJA7>3.0.CO;2-D
Van Den Brûle, Frédéric A. ; Fernandez, Pedro L. ; Buicu, Crina ; Liu, Fu-Tong ; Jackers, Pascale ; Lambotte, René ; Castronovo, Vincent. / Differential expression of galectin-1 and galectin-3 during first trimester human embryogenesis. In: Developmental Dynamics. 1997 ; Vol. 209, No. 4. pp. 399-405.
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