Differential expression of bovine MHC class II antigens identified by monoclonal antibodies

B. C. Taylor, K. Y. Choi, R. J. Scibienski, Peter F Moore, Jeffrey L Stott

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Four monoclonal antibodies (mAbs), UC-A4, UC-D3, UC-H9, and IL-A21, specific for bovine major histocompatibility complex class II proteins are described. Sequential immunoprecipitation experiments using biotin-labeled peripheral blood mononuclear cells suggested, but did not conclusively establish, that each of these antibodies recognized a different epitope. The epitope identified by IL-A21 appeared to be common to all of the class II proteins precipitated by the four mAbs, and UC-D3 and UC-H9 each appeared to react with distinct epitopes on separate subsets of these class II proteins. Monoclonal antibody UC-A4 appeared to identify an epitope on a subset of the class II molecules identified by UC-H9. Differences found in the expression by lymphoid cells of class II proteins identified by the four mAbs were indicative of each mAb recognizing a different epitope. UC-H9 and IL-A21 class II proteins were detected on all surface immunoglobulin (S'Ig) positive cells in peripheral blood, but UC-A4 and UC-D3 class II proteins were not. Expression of UC-A4 class II proteins, detected at low density on a strikingly reduced number of S'Ig+ cells from the blood of some bovine leukosis virus-infected cattle, could be increased by culturing these B cells with lipopolysaccharide. All peripheral blood monocytes expressed UC-H9 and IL-A21 class II proteins, but only a proportion of monocytes expressed detectable UC-A4 and UC-D3 class II proteins. Almost all mitogen-stimulated BoCD4+ and BoCD8+ T cells expressed UC-H9 and IL-A21 class II proteins, whereas fewer stimulated T cells of both subsets expressed UC-A4 and UC-D3 class II proteins. All gamma/delta receptor (γ/δ R) T cells expressed UC- D3, UC-H9, and IL-A21 class II proteins, but no cells (of γ/δ R+ or CD4+/CD2+ phenotype) from γ/δ R+ T cell-enriched cultures expressed UC- A4 class II proteins.

Original languageEnglish (US)
Pages (from-to)479-489
Number of pages11
JournalJournal of Leukocyte Biology
Volume53
Issue number5
StatePublished - 1993

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Histocompatibility Antigens Class II
Monoclonal Antibodies
Proteins
Epitopes
B-Cell Antigen Receptors
Monocytes
Blood Cells
Antigen Receptors, T-Cell, gamma-delta
Enzootic Bovine Leukosis
T-Lymphocytes
T-Lymphocyte Subsets
Biotin
Major Histocompatibility Complex
Mitogens
Immunoprecipitation
Lipopolysaccharides
B-Lymphocytes
Cell Culture Techniques

Keywords

  • B cells
  • BoLA D
  • monoclonal antibodies
  • monocytes
  • T cells

ASJC Scopus subject areas

  • Cell Biology

Cite this

Differential expression of bovine MHC class II antigens identified by monoclonal antibodies. / Taylor, B. C.; Choi, K. Y.; Scibienski, R. J.; Moore, Peter F; Stott, Jeffrey L.

In: Journal of Leukocyte Biology, Vol. 53, No. 5, 1993, p. 479-489.

Research output: Contribution to journalArticle

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abstract = "Four monoclonal antibodies (mAbs), UC-A4, UC-D3, UC-H9, and IL-A21, specific for bovine major histocompatibility complex class II proteins are described. Sequential immunoprecipitation experiments using biotin-labeled peripheral blood mononuclear cells suggested, but did not conclusively establish, that each of these antibodies recognized a different epitope. The epitope identified by IL-A21 appeared to be common to all of the class II proteins precipitated by the four mAbs, and UC-D3 and UC-H9 each appeared to react with distinct epitopes on separate subsets of these class II proteins. Monoclonal antibody UC-A4 appeared to identify an epitope on a subset of the class II molecules identified by UC-H9. Differences found in the expression by lymphoid cells of class II proteins identified by the four mAbs were indicative of each mAb recognizing a different epitope. UC-H9 and IL-A21 class II proteins were detected on all surface immunoglobulin (S'Ig) positive cells in peripheral blood, but UC-A4 and UC-D3 class II proteins were not. Expression of UC-A4 class II proteins, detected at low density on a strikingly reduced number of S'Ig+ cells from the blood of some bovine leukosis virus-infected cattle, could be increased by culturing these B cells with lipopolysaccharide. All peripheral blood monocytes expressed UC-H9 and IL-A21 class II proteins, but only a proportion of monocytes expressed detectable UC-A4 and UC-D3 class II proteins. Almost all mitogen-stimulated BoCD4+ and BoCD8+ T cells expressed UC-H9 and IL-A21 class II proteins, whereas fewer stimulated T cells of both subsets expressed UC-A4 and UC-D3 class II proteins. All gamma/delta receptor (γ/δ R) T cells expressed UC- D3, UC-H9, and IL-A21 class II proteins, but no cells (of γ/δ R+ or CD4+/CD2+ phenotype) from γ/δ R+ T cell-enriched cultures expressed UC- A4 class II proteins.",
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