Differential effects of infection with a Babesia-like piroplasm, WA1, in inbred mice

Manuel H. Moro, Chella S. David, Jenifer M. Magera, Peter J. Wettstein, Stephen W Barthold, David H. Persing

Research output: Contribution to journalArticle

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Abstract

A newly identified intraerythrocytic Babesia-like organism, WA1, and its relatives were recently shown to be infectious for humans in the western United States. The purpose of the present study was to determine the susceptibilities of selected mouse genotypes to WA1 infection in an attempt to develop a murine model of the human disease. Several mouse strains were inoculated intraperitoneally with various passages of WA1-parasitized erythrocytes. Parasitemia was evaluated by blood smears and by PCR with blood samples collected at various intervals after inoculation. Hematologic parameters were monitored in blood samples at all intervals. C57BL/6 and C57BL/10 mice exhibited mortality rates of < 10%. BALB/cJ, CBAJ, and 129/J mice had higher peak parasitemias than did C57BL mice, with mortality rates of 40, 50, and 50%, respectively. A/J, AKR/N, C3H, and DBA/1J mice also had higher peak parasitemia and mortality rates (>95%). An F1 cross of C57BL/6 (resistant) and C3H.RKK (susceptible) mice had a mortality rate similar to that of the resistant parental strain. Histopathology of BALB/cJ and C3H mice at 9 and 14 days after inoculation revealed erythrophagocytosis and deposition of an iron-negative pigment in multiple organs. Morbidly ill C3H mice at 14 days had severe pulmonary edema, hemoglobinuria, and glomerulonephritis.

Original languageEnglish (US)
Pages (from-to)492-498
Number of pages7
JournalInfection and Immunity
Volume66
Issue number2
StatePublished - 1998

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Babesia
Inbred C3H Mouse
Infection
Hemoglobinuria
Parasitemia
Mortality
Pulmonary Edema
Glomerulonephritis
Inbred C57BL Mouse
Iron
Erythrocytes
Genotype
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Immunology

Cite this

Moro, M. H., David, C. S., Magera, J. M., Wettstein, P. J., Barthold, S. W., & Persing, D. H. (1998). Differential effects of infection with a Babesia-like piroplasm, WA1, in inbred mice. Infection and Immunity, 66(2), 492-498.

Differential effects of infection with a Babesia-like piroplasm, WA1, in inbred mice. / Moro, Manuel H.; David, Chella S.; Magera, Jenifer M.; Wettstein, Peter J.; Barthold, Stephen W; Persing, David H.

In: Infection and Immunity, Vol. 66, No. 2, 1998, p. 492-498.

Research output: Contribution to journalArticle

Moro, MH, David, CS, Magera, JM, Wettstein, PJ, Barthold, SW & Persing, DH 1998, 'Differential effects of infection with a Babesia-like piroplasm, WA1, in inbred mice', Infection and Immunity, vol. 66, no. 2, pp. 492-498.
Moro, Manuel H. ; David, Chella S. ; Magera, Jenifer M. ; Wettstein, Peter J. ; Barthold, Stephen W ; Persing, David H. / Differential effects of infection with a Babesia-like piroplasm, WA1, in inbred mice. In: Infection and Immunity. 1998 ; Vol. 66, No. 2. pp. 492-498.
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