Differential effects of docosahexaenoic acid and eicosapentaenoic acid on suppression of lipoxygenase pathway in peritoneal macrophages

B. R. Lokesh; B. German; J. E. Kinsella

doi:10.1016/0005-2760(88)90250-0

Differential effects of docosahexaenoic acid and eicosapentaenoic acid on suppression of lipoxygenase pathway in peritoneal macrophages

B. R. Lokesh, B. German, J. E. Kinsella

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

Docosahexaenoic acid (DHA) or eicosapentaenoic acid (EPA) was facilely incorporated into phospholipids of mouse peritoneal macrophages following incubation with pure fatty acids complexed to bovine serum albumin. Following stimulation with calcium ionophore A23187, the DHA-enriched cells synthesized significantly smaller amounts of leukotriene C₄ and leukotriene B₄ compared to control or EPA-enriched cells. The EPA-enriched cells synthesized lower amounts of leukotriene C₄ and leukotriene B₄ compared to control cells. The stimulated macrophages utilized endogenously released arachidonic acid for leukotriene B₄ and leukotriene C₄ synthesis. Exogenous arachidonic acid increased the formation of 12-hydroxyeico-satetraenoic acid (12-HETE) and 15-HETE and macrophages enriched with DHA or EPA produced similar amounts of 12-HETE and 15-HETE compared to control cells. These studies demonstrated that the synthesis of leukotriene C₄, leukotriene B₄ and HETE in macrophages is differentially affected by DHA and EPA.

Original language	English (US)
Pages (from-to)	99-107
Number of pages	9
Journal	Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume	958
Issue number	1
DOIs	https://doi.org/10.1016/0005-2760(88)90250-0
State	Published - Jan 19 1988
Externally published	Yes

Keywords

(Mouse macrophage)
Docosahexaenoic acid
Icosapentaenoic acid
Leukotriene synthesis

ASJC Scopus subject areas

Biochemistry
Biophysics
Endocrinology

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10.1016/0005-2760(88)90250-0

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abstract = "Docosahexaenoic acid (DHA) or eicosapentaenoic acid (EPA) was facilely incorporated into phospholipids of mouse peritoneal macrophages following incubation with pure fatty acids complexed to bovine serum albumin. Following stimulation with calcium ionophore A23187, the DHA-enriched cells synthesized significantly smaller amounts of leukotriene C4 and leukotriene B4 compared to control or EPA-enriched cells. The EPA-enriched cells synthesized lower amounts of leukotriene C4 and leukotriene B4 compared to control cells. The stimulated macrophages utilized endogenously released arachidonic acid for leukotriene B4 and leukotriene C4 synthesis. Exogenous arachidonic acid increased the formation of 12-hydroxyeico-satetraenoic acid (12-HETE) and 15-HETE and macrophages enriched with DHA or EPA produced similar amounts of 12-HETE and 15-HETE compared to control cells. These studies demonstrated that the synthesis of leukotriene C4, leukotriene B4 and HETE in macrophages is differentially affected by DHA and EPA.",

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year = "1988",

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AU - German, B.

AU - Kinsella, J. E.

PY - 1988/1/19

Y1 - 1988/1/19

N2 - Docosahexaenoic acid (DHA) or eicosapentaenoic acid (EPA) was facilely incorporated into phospholipids of mouse peritoneal macrophages following incubation with pure fatty acids complexed to bovine serum albumin. Following stimulation with calcium ionophore A23187, the DHA-enriched cells synthesized significantly smaller amounts of leukotriene C4 and leukotriene B4 compared to control or EPA-enriched cells. The EPA-enriched cells synthesized lower amounts of leukotriene C4 and leukotriene B4 compared to control cells. The stimulated macrophages utilized endogenously released arachidonic acid for leukotriene B4 and leukotriene C4 synthesis. Exogenous arachidonic acid increased the formation of 12-hydroxyeico-satetraenoic acid (12-HETE) and 15-HETE and macrophages enriched with DHA or EPA produced similar amounts of 12-HETE and 15-HETE compared to control cells. These studies demonstrated that the synthesis of leukotriene C4, leukotriene B4 and HETE in macrophages is differentially affected by DHA and EPA.

AB - Docosahexaenoic acid (DHA) or eicosapentaenoic acid (EPA) was facilely incorporated into phospholipids of mouse peritoneal macrophages following incubation with pure fatty acids complexed to bovine serum albumin. Following stimulation with calcium ionophore A23187, the DHA-enriched cells synthesized significantly smaller amounts of leukotriene C4 and leukotriene B4 compared to control or EPA-enriched cells. The EPA-enriched cells synthesized lower amounts of leukotriene C4 and leukotriene B4 compared to control cells. The stimulated macrophages utilized endogenously released arachidonic acid for leukotriene B4 and leukotriene C4 synthesis. Exogenous arachidonic acid increased the formation of 12-hydroxyeico-satetraenoic acid (12-HETE) and 15-HETE and macrophages enriched with DHA or EPA produced similar amounts of 12-HETE and 15-HETE compared to control cells. These studies demonstrated that the synthesis of leukotriene C4, leukotriene B4 and HETE in macrophages is differentially affected by DHA and EPA.

KW - (Mouse macrophage)

KW - Docosahexaenoic acid

KW - Icosapentaenoic acid

KW - Leukotriene synthesis

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Differential effects of docosahexaenoic acid and eicosapentaenoic acid on suppression of lipoxygenase pathway in peritoneal macrophages

Abstract

Keywords

ASJC Scopus subject areas

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