Abstract
Objectives: As interleukin (IL)-2 therapy increases CD4 cell counts in HIV infected subjects, it emerged as a candidate for the partial restoration of immune competence in this disease. Methods: We studied the frequencies of antigen-specific T cells using single cell resolution cytokine ELISPOT assays and titers of specific antibodies before and after immunization of HIV infected subjects who were treated with HAART or HAART plus IL-2. Results: Subjects seronegative to hepatitis A were vaccinated with hepatitis A antigen. In the non-IL-2 treated group, hepatitis A-specific T cells producing IL-2 and IL-4 along with specific antibodies were induced, showing that these subjects are immune competent and capable of mounting a primary immune response. Additional IL-2 treatment had no significant effect on this primary T cell response; however, booster immunizations with tetanus toxoid or the gp120 depleted HIV vaccine Remune induced higher frequencies of specific interferon (IFN)-γ producing T cells in IL-2 treated subjects. No impact of IL-2 treatment on these secondary B cell responses was seen. Conclusion: Overall, our study showed that IL-2 therapy had no immune enhancing effect on the induction of a primary response, but increased the frequency of IFN-γ producing memory cells after booster immunization.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1967-1974 |
| Number of pages | 8 |
| Journal | AIDS |
| Volume | 19 |
| Issue number | 17 |
| State | Published - Nov 2005 |
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Keywords
- AIDS vaccine
- B cells
- Cytokines
- ELISPOT
- HIV
- Th1 response
- Th2 response
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
Cite this
Differential effect of interleukin-2 treatment on primary and secondary immunizations in HIV infected individuals. / Kuekrek, Haydar; Schlingmann, Tobias; Valdez, Hernan; Boehm, Bernhard O.; Pollard, Richard B; Mitsuyasu, Ronald; Goebel, Frank Detlef; Lederman, Michael M.; Lehmann, Paul V.; Tary-Lehmann, Magdalena.
In: AIDS, Vol. 19, No. 17, 11.2005, p. 1967-1974.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Differential effect of interleukin-2 treatment on primary and secondary immunizations in HIV infected individuals
AU - Kuekrek, Haydar
AU - Schlingmann, Tobias
AU - Valdez, Hernan
AU - Boehm, Bernhard O.
AU - Pollard, Richard B
AU - Mitsuyasu, Ronald
AU - Goebel, Frank Detlef
AU - Lederman, Michael M.
AU - Lehmann, Paul V.
AU - Tary-Lehmann, Magdalena
PY - 2005/11
Y1 - 2005/11
N2 - Objectives: As interleukin (IL)-2 therapy increases CD4 cell counts in HIV infected subjects, it emerged as a candidate for the partial restoration of immune competence in this disease. Methods: We studied the frequencies of antigen-specific T cells using single cell resolution cytokine ELISPOT assays and titers of specific antibodies before and after immunization of HIV infected subjects who were treated with HAART or HAART plus IL-2. Results: Subjects seronegative to hepatitis A were vaccinated with hepatitis A antigen. In the non-IL-2 treated group, hepatitis A-specific T cells producing IL-2 and IL-4 along with specific antibodies were induced, showing that these subjects are immune competent and capable of mounting a primary immune response. Additional IL-2 treatment had no significant effect on this primary T cell response; however, booster immunizations with tetanus toxoid or the gp120 depleted HIV vaccine Remune induced higher frequencies of specific interferon (IFN)-γ producing T cells in IL-2 treated subjects. No impact of IL-2 treatment on these secondary B cell responses was seen. Conclusion: Overall, our study showed that IL-2 therapy had no immune enhancing effect on the induction of a primary response, but increased the frequency of IFN-γ producing memory cells after booster immunization.
AB - Objectives: As interleukin (IL)-2 therapy increases CD4 cell counts in HIV infected subjects, it emerged as a candidate for the partial restoration of immune competence in this disease. Methods: We studied the frequencies of antigen-specific T cells using single cell resolution cytokine ELISPOT assays and titers of specific antibodies before and after immunization of HIV infected subjects who were treated with HAART or HAART plus IL-2. Results: Subjects seronegative to hepatitis A were vaccinated with hepatitis A antigen. In the non-IL-2 treated group, hepatitis A-specific T cells producing IL-2 and IL-4 along with specific antibodies were induced, showing that these subjects are immune competent and capable of mounting a primary immune response. Additional IL-2 treatment had no significant effect on this primary T cell response; however, booster immunizations with tetanus toxoid or the gp120 depleted HIV vaccine Remune induced higher frequencies of specific interferon (IFN)-γ producing T cells in IL-2 treated subjects. No impact of IL-2 treatment on these secondary B cell responses was seen. Conclusion: Overall, our study showed that IL-2 therapy had no immune enhancing effect on the induction of a primary response, but increased the frequency of IFN-γ producing memory cells after booster immunization.
KW - AIDS vaccine
KW - B cells
KW - Cytokines
KW - ELISPOT
KW - HIV
KW - Th1 response
KW - Th2 response
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UR - http://www.scopus.com/inward/citedby.url?scp=27944487962&partnerID=8YFLogxK
M3 - Article
C2 - 16260902
AN - SCOPUS:27944487962
VL - 19
SP - 1967
EP - 1974
JO - AIDS
JF - AIDS
SN - 0269-9370
IS - 17
ER -