Different physicochemical, structural and digestibility characteristics of myofibrillar protein from PSE and normal pork before and after oxidation

Lin Chen, Chenyi Li, Niamat Ullah, Yan Guo, Xuchun Sun, Xuejiao Wang, Xinglian Xu, Robert M. Hackman, Guanghong Zhou, Xianchao Feng

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

PSE pork has a weaker texture than normal pork due to the denaturation by endogenous protease. Changes in the nutritional quality and characteristics of myofibrillar protein (MP) from PSE pork have been scarcely documented. MPs were isolated from both PSE (MPP) and normal pork (MPN) and were oxidized for 12 h at 4 °C by a hydroxyl radical generating system (10 μM FeCl3, 100 μM ascorbic acid, 0.1, 1, and 10 mM H2O2). The MPP had less aggregation and looser structure with higher surface hydrophobicity. The MPP was also less polymeric, as evidenced by intenser bands on SDS-PAGE and fewer carbonyl–NH2 interactions. The MPP particles were of smaller size, caused by the endogenous protease and oxidation, and reached the critical concentration to enhance the solubility. The altered characteristics of MPP enhanced its in vitro digestion rate and overall digestibility. In addition, the in vitro digestion rate of MPN can be enhanced if the oxidative stress is strong enough (10 mM H2O2).

Original languageEnglish (US)
Pages (from-to)228-237
Number of pages10
JournalMeat Science
Volume121
DOIs
StatePublished - Nov 1 2016
Externally publishedYes

Fingerprint

myofibrillar proteins
pork
digestibility
oxidation
in vitro digestion
Proteins
Digestion
Peptide Hydrolases
proteinases
nutritive value
Nutritive Value
hydroxyl radicals
hydrophobicity
denaturation
Hydrophobic and Hydrophilic Interactions
Hydroxyl Radical
Solubility
Ascorbic Acid
solubility
Polyacrylamide Gel Electrophoresis

Keywords

  • In vitro digestion
  • Myofibrillar proteins
  • Nutritional quality
  • Oxidation
  • PSE

ASJC Scopus subject areas

  • Food Science

Cite this

Different physicochemical, structural and digestibility characteristics of myofibrillar protein from PSE and normal pork before and after oxidation. / Chen, Lin; Li, Chenyi; Ullah, Niamat; Guo, Yan; Sun, Xuchun; Wang, Xuejiao; Xu, Xinglian; Hackman, Robert M.; Zhou, Guanghong; Feng, Xianchao.

In: Meat Science, Vol. 121, 01.11.2016, p. 228-237.

Research output: Contribution to journalArticle

Chen, Lin ; Li, Chenyi ; Ullah, Niamat ; Guo, Yan ; Sun, Xuchun ; Wang, Xuejiao ; Xu, Xinglian ; Hackman, Robert M. ; Zhou, Guanghong ; Feng, Xianchao. / Different physicochemical, structural and digestibility characteristics of myofibrillar protein from PSE and normal pork before and after oxidation. In: Meat Science. 2016 ; Vol. 121. pp. 228-237.
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