Different apolipoproteins impact nanolipoprotein particle formation

Brett A. Chromy, Erin Arroyo, Craig D. Blanchette, Graham Bench, Henry Benner, Jenny A. Cappuccio, Matthew A Coleman, Paul Henderson, Angie K. Hinz, Edward A. Kuhn, Joseph B. Pesavento, Brent W. Segelke, Todd A. Sulchek, Ted Tarasow, Vicki L. Walsworth, Paul D. Hoeprich

Research output: Contribution to journalArticle

65 Scopus citations

Abstract

Spontaneous interaction of purified apolipoproteins and phospholipids results in formation of lipoprotein particles with nanometer-sized dimensions; we refer to these assemblies as nanolipoprotein particles or NLPs. These bilayer constructs can serve as suitable mimetics of biological membranes and are fully soluble in aqueous environments. We made NLPs from dimyristoylphospatidylcholine (DMPC) in combination with each of four different apolipoproteins: apoA-I, Δ-apoA-I fragment, apoE4 fragment, and apolipophorin III (apoLp-III) from the silk moth B. mori. Predominately discoidal in shape, these particles have diameters between 10 and 20 nm, share uniform heights between 4.5 and 5 nm, and can be produced in yields ranging between 40 and 60%. The particular lipoprotein, the lipid to lipoprotein ratio, and the assembly parameters determine the size and homogeneity of nanolipoprotein particles and indicate that apoA-I NLP preparations are smaller than the larger apoE422K and apoLp-III NLP preparations.

Original languageEnglish (US)
Pages (from-to)14348-14354
Number of pages7
JournalJournal of the American Chemical Society
Volume129
Issue number46
DOIs
StatePublished - Nov 21 2007
Externally publishedYes

ASJC Scopus subject areas

  • Chemistry(all)

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    Chromy, B. A., Arroyo, E., Blanchette, C. D., Bench, G., Benner, H., Cappuccio, J. A., Coleman, M. A., Henderson, P., Hinz, A. K., Kuhn, E. A., Pesavento, J. B., Segelke, B. W., Sulchek, T. A., Tarasow, T., Walsworth, V. L., & Hoeprich, P. D. (2007). Different apolipoproteins impact nanolipoprotein particle formation. Journal of the American Chemical Society, 129(46), 14348-14354. https://doi.org/10.1021/ja074753y