Abstract
Feline infectious peritonitis viruses (FIPVs) are classified into type I and type II serogroups. Here, we report that feline aminopeptidase N (APN), a cell-surface metalloprotease on the intestinal, lung and kidney epithelial cells, is a receptor for type II FIPV but not for type I FIPV. A monoclonal antibody (MAb) R-G-4, which blocks infection of Fells cams whole fetus (fcwf-4) cells by type II FIPV, was obtained by immunizing mice with fcwf-4 cells, which are highly susceptible to FIPV. This MAb also blocked infection of fcwf-4 cells by type II feline enteric coronavirus (FECV), canine coronavirus (CCV), and transmissible gastroenteritis virus (TGEV). On the other hand, it did not block infection by type I FIPVs. MAb R-G-4 recognized a polypeptide of relative molecular mass 120-130 kDa in feline intestinal brush-border membrane (BBM) proteins. The polypeptide possessed aminopeptidase activity, and the first 15 N-terminal amino acid sequence was identical to that of the feline APN. Feline intestinal BBM proteins and the polypeptide reacted with MAb R-G-4 (feline APN) inhibited the infectivity of type II FIPV, type II FECV, CCV and TGEV to fcwf-4 cells, but did not inhibit the infectivity of type I FIPVs.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 839-850 |
| Number of pages | 12 |
| Journal | Archives of Virology |
| Volume | 143 |
| Issue number | 5 |
| DOIs | |
| State | Published - 1998 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Genetics
- Applied Microbiology and Biotechnology
Cite this
Differences in virus receptor for type I and type II feline infectious peritonitis virus. / Hohdatsu, T.; Izumiya, Yoshihiro; Yokoyama, Y.; Kida, K.; Koyama, H.
In: Archives of Virology, Vol. 143, No. 5, 1998, p. 839-850.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Differences in virus receptor for type I and type II feline infectious peritonitis virus
AU - Hohdatsu, T.
AU - Izumiya, Yoshihiro
AU - Yokoyama, Y.
AU - Kida, K.
AU - Koyama, H.
PY - 1998
Y1 - 1998
N2 - Feline infectious peritonitis viruses (FIPVs) are classified into type I and type II serogroups. Here, we report that feline aminopeptidase N (APN), a cell-surface metalloprotease on the intestinal, lung and kidney epithelial cells, is a receptor for type II FIPV but not for type I FIPV. A monoclonal antibody (MAb) R-G-4, which blocks infection of Fells cams whole fetus (fcwf-4) cells by type II FIPV, was obtained by immunizing mice with fcwf-4 cells, which are highly susceptible to FIPV. This MAb also blocked infection of fcwf-4 cells by type II feline enteric coronavirus (FECV), canine coronavirus (CCV), and transmissible gastroenteritis virus (TGEV). On the other hand, it did not block infection by type I FIPVs. MAb R-G-4 recognized a polypeptide of relative molecular mass 120-130 kDa in feline intestinal brush-border membrane (BBM) proteins. The polypeptide possessed aminopeptidase activity, and the first 15 N-terminal amino acid sequence was identical to that of the feline APN. Feline intestinal BBM proteins and the polypeptide reacted with MAb R-G-4 (feline APN) inhibited the infectivity of type II FIPV, type II FECV, CCV and TGEV to fcwf-4 cells, but did not inhibit the infectivity of type I FIPVs.
AB - Feline infectious peritonitis viruses (FIPVs) are classified into type I and type II serogroups. Here, we report that feline aminopeptidase N (APN), a cell-surface metalloprotease on the intestinal, lung and kidney epithelial cells, is a receptor for type II FIPV but not for type I FIPV. A monoclonal antibody (MAb) R-G-4, which blocks infection of Fells cams whole fetus (fcwf-4) cells by type II FIPV, was obtained by immunizing mice with fcwf-4 cells, which are highly susceptible to FIPV. This MAb also blocked infection of fcwf-4 cells by type II feline enteric coronavirus (FECV), canine coronavirus (CCV), and transmissible gastroenteritis virus (TGEV). On the other hand, it did not block infection by type I FIPVs. MAb R-G-4 recognized a polypeptide of relative molecular mass 120-130 kDa in feline intestinal brush-border membrane (BBM) proteins. The polypeptide possessed aminopeptidase activity, and the first 15 N-terminal amino acid sequence was identical to that of the feline APN. Feline intestinal BBM proteins and the polypeptide reacted with MAb R-G-4 (feline APN) inhibited the infectivity of type II FIPV, type II FECV, CCV and TGEV to fcwf-4 cells, but did not inhibit the infectivity of type I FIPVs.
UR - http://www.scopus.com/inward/record.url?scp=0031832221&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031832221&partnerID=8YFLogxK
U2 - 10.1007/s007050050336
DO - 10.1007/s007050050336
M3 - Article
C2 - 9645192
AN - SCOPUS:0031832221
VL - 143
SP - 839
EP - 850
JO - Archives of Virology
JF - Archives of Virology
SN - 0304-8608
IS - 5
ER -