Differences in virus receptor for type I and type II feline infectious peritonitis virus

T. Hohdatsu, Yoshihiro Izumiya, Y. Yokoyama, K. Kida, H. Koyama

Research output: Contribution to journalArticle

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Abstract

Feline infectious peritonitis viruses (FIPVs) are classified into type I and type II serogroups. Here, we report that feline aminopeptidase N (APN), a cell-surface metalloprotease on the intestinal, lung and kidney epithelial cells, is a receptor for type II FIPV but not for type I FIPV. A monoclonal antibody (MAb) R-G-4, which blocks infection of Fells cams whole fetus (fcwf-4) cells by type II FIPV, was obtained by immunizing mice with fcwf-4 cells, which are highly susceptible to FIPV. This MAb also blocked infection of fcwf-4 cells by type II feline enteric coronavirus (FECV), canine coronavirus (CCV), and transmissible gastroenteritis virus (TGEV). On the other hand, it did not block infection by type I FIPVs. MAb R-G-4 recognized a polypeptide of relative molecular mass 120-130 kDa in feline intestinal brush-border membrane (BBM) proteins. The polypeptide possessed aminopeptidase activity, and the first 15 N-terminal amino acid sequence was identical to that of the feline APN. Feline intestinal BBM proteins and the polypeptide reacted with MAb R-G-4 (feline APN) inhibited the infectivity of type II FIPV, type II FECV, CCV and TGEV to fcwf-4 cells, but did not inhibit the infectivity of type I FIPVs.

Original languageEnglish (US)
Pages (from-to)839-850
Number of pages12
JournalArchives of Virology
Volume143
Issue number5
DOIs
StatePublished - 1998
Externally publishedYes

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Feline Coronavirus
Virus Receptors
Felidae
CD13 Antigens
Canine Coronavirus
Transmissible gastroenteritis virus
Monoclonal Antibodies
Microvilli
Peptides
Membrane Proteins
Infection
Aminopeptidases
Metalloproteases
Amino Acid Sequence
Fetus

ASJC Scopus subject areas

  • Genetics
  • Applied Microbiology and Biotechnology

Cite this

Differences in virus receptor for type I and type II feline infectious peritonitis virus. / Hohdatsu, T.; Izumiya, Yoshihiro; Yokoyama, Y.; Kida, K.; Koyama, H.

In: Archives of Virology, Vol. 143, No. 5, 1998, p. 839-850.

Research output: Contribution to journalArticle

Hohdatsu, T. ; Izumiya, Yoshihiro ; Yokoyama, Y. ; Kida, K. ; Koyama, H. / Differences in virus receptor for type I and type II feline infectious peritonitis virus. In: Archives of Virology. 1998 ; Vol. 143, No. 5. pp. 839-850.
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