Differences in the expression of cytochromes P450 17α-hydroxylase and aromatase in Meishan and Yorkshire conceptuses at days 10.5-14.0 of gestation

M. A. Kaminski, S. P. Ford, Alan J Conley

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The expression of cytochromes P450 17α-hydroxylase (P450c17) and (P450arom) was compared between preimplantation Chinese Meishan and domestic Yorkshire conceptuses during the period encompassing maternal recognition of pregnancy. Individual conceptuses were recovered on days 10.5, 11.0, 11.5, 12.0, and 14.0 of gestation. Diameter (spherical blastocysts), length (elongated blastocysts), DNA, protein and oestradiol content, as well as the amounts of P450c17 and P450arom (western analysis) were determined in individual conceptuses. Comparisons were made only between conceptuses of similar diameters on each day which restricted analyses to blastocysts 6 mm or less in diameter on days 10.5-12.0. Nonetheless, both DNA and protein content were greater in Yorkshire than in Meishan conceptuses. Oestradiol content also tended to be greater in Yorkshire than in Meishan conceptuses across days. A significant effect of breed and breed by day interaction was detected for P450c17. Expression of P450c17 in Yorkshire conceptuses increased markedly above that in Meishan conceptuses by day 11, remained high until day 11.5 and returned to values similar to those of Meishans by day 12. The expression of P450arom was also greater in Yorkshire than in Meishan conceptuses, but no breed by day interaction was detected. These data suggest that differences in development between Meishan and Yorkshire conceptuses include trophoblastic differentiation during preattachment stages. The significance and impact of this divergence in development on subsequent growth and survival remains to be determined.

Original languageEnglish (US)
Pages (from-to)213-219
Number of pages7
JournalJournal of Reproduction and Fertility
Volume111
Issue number2
StatePublished - Nov 1997

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Aromatase
Blastocyst
Mixed Function Oxygenases
Cytochrome P-450 Enzyme System
Pregnancy
Estradiol
DNA
Proteins
Mothers
Growth

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Embryology
  • Developmental Biology
  • Molecular Biology
  • Physiology
  • Cell Biology
  • Endocrinology

Cite this

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title = "Differences in the expression of cytochromes P450 17α-hydroxylase and aromatase in Meishan and Yorkshire conceptuses at days 10.5-14.0 of gestation",
abstract = "The expression of cytochromes P450 17α-hydroxylase (P450c17) and (P450arom) was compared between preimplantation Chinese Meishan and domestic Yorkshire conceptuses during the period encompassing maternal recognition of pregnancy. Individual conceptuses were recovered on days 10.5, 11.0, 11.5, 12.0, and 14.0 of gestation. Diameter (spherical blastocysts), length (elongated blastocysts), DNA, protein and oestradiol content, as well as the amounts of P450c17 and P450arom (western analysis) were determined in individual conceptuses. Comparisons were made only between conceptuses of similar diameters on each day which restricted analyses to blastocysts 6 mm or less in diameter on days 10.5-12.0. Nonetheless, both DNA and protein content were greater in Yorkshire than in Meishan conceptuses. Oestradiol content also tended to be greater in Yorkshire than in Meishan conceptuses across days. A significant effect of breed and breed by day interaction was detected for P450c17. Expression of P450c17 in Yorkshire conceptuses increased markedly above that in Meishan conceptuses by day 11, remained high until day 11.5 and returned to values similar to those of Meishans by day 12. The expression of P450arom was also greater in Yorkshire than in Meishan conceptuses, but no breed by day interaction was detected. These data suggest that differences in development between Meishan and Yorkshire conceptuses include trophoblastic differentiation during preattachment stages. The significance and impact of this divergence in development on subsequent growth and survival remains to be determined.",
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T1 - Differences in the expression of cytochromes P450 17α-hydroxylase and aromatase in Meishan and Yorkshire conceptuses at days 10.5-14.0 of gestation

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AU - Ford, S. P.

AU - Conley, Alan J

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N2 - The expression of cytochromes P450 17α-hydroxylase (P450c17) and (P450arom) was compared between preimplantation Chinese Meishan and domestic Yorkshire conceptuses during the period encompassing maternal recognition of pregnancy. Individual conceptuses were recovered on days 10.5, 11.0, 11.5, 12.0, and 14.0 of gestation. Diameter (spherical blastocysts), length (elongated blastocysts), DNA, protein and oestradiol content, as well as the amounts of P450c17 and P450arom (western analysis) were determined in individual conceptuses. Comparisons were made only between conceptuses of similar diameters on each day which restricted analyses to blastocysts 6 mm or less in diameter on days 10.5-12.0. Nonetheless, both DNA and protein content were greater in Yorkshire than in Meishan conceptuses. Oestradiol content also tended to be greater in Yorkshire than in Meishan conceptuses across days. A significant effect of breed and breed by day interaction was detected for P450c17. Expression of P450c17 in Yorkshire conceptuses increased markedly above that in Meishan conceptuses by day 11, remained high until day 11.5 and returned to values similar to those of Meishans by day 12. The expression of P450arom was also greater in Yorkshire than in Meishan conceptuses, but no breed by day interaction was detected. These data suggest that differences in development between Meishan and Yorkshire conceptuses include trophoblastic differentiation during preattachment stages. The significance and impact of this divergence in development on subsequent growth and survival remains to be determined.

AB - The expression of cytochromes P450 17α-hydroxylase (P450c17) and (P450arom) was compared between preimplantation Chinese Meishan and domestic Yorkshire conceptuses during the period encompassing maternal recognition of pregnancy. Individual conceptuses were recovered on days 10.5, 11.0, 11.5, 12.0, and 14.0 of gestation. Diameter (spherical blastocysts), length (elongated blastocysts), DNA, protein and oestradiol content, as well as the amounts of P450c17 and P450arom (western analysis) were determined in individual conceptuses. Comparisons were made only between conceptuses of similar diameters on each day which restricted analyses to blastocysts 6 mm or less in diameter on days 10.5-12.0. Nonetheless, both DNA and protein content were greater in Yorkshire than in Meishan conceptuses. Oestradiol content also tended to be greater in Yorkshire than in Meishan conceptuses across days. A significant effect of breed and breed by day interaction was detected for P450c17. Expression of P450c17 in Yorkshire conceptuses increased markedly above that in Meishan conceptuses by day 11, remained high until day 11.5 and returned to values similar to those of Meishans by day 12. The expression of P450arom was also greater in Yorkshire than in Meishan conceptuses, but no breed by day interaction was detected. These data suggest that differences in development between Meishan and Yorkshire conceptuses include trophoblastic differentiation during preattachment stages. The significance and impact of this divergence in development on subsequent growth and survival remains to be determined.

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