Differences in exon expression and alternatively spliced genes in blood of multiple sclerosis compared to healthy control subjects

Yingfang Tian; Michelle L Apperson; Bradley Ander; Da Liu; Boryana S. Stomova; Glen C. Jickling; Richelle Enriquez; Mark A. Agius; Frank R Sharp

doi:10.1016/j.jneuroim.2010.09.001

Differences in exon expression and alternatively spliced genes in blood of multiple sclerosis compared to healthy control subjects

Yingfang Tian, Michelle L Apperson, Bradley Ander, Da Liu, Boryana S. Stomova, Glen C. Jickling, Richelle Enriquez, Mark A. Agius, Frank R Sharp

Neurology

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Using whole genome exon microarrays 120 exons were differentially expressed between medication-free multiple sclerosis (MS) subjects in remission and healthy control subjects (HS) (p < 0.001, fold change > |1.2|). These exons differentiated MS from HS using cluster analyses, principal components analyses (PCAs) and cross-validation. In addition, 340 genes (transcripts) were predicted to be alternatively spliced in MS compared to HS. These findings may provide insight into the pathophysiology of MS and potentially provide prognostic and diagnostic biomarkers. However, given that multiple comparisons were performed on a very small sample, these preliminary findings require confirmation using a much larger independent cohort.

Original language	English (US)
Pages (from-to)	124-129
Number of pages	6
Journal	Journal of Neuroimmunology
Volume	230
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jneuroim.2010.09.001
State	Published - Jan 2011

Keywords

Alternative splicing
Blood biomarker
Exon expression profiles
Gene expression profiling
Multiple sclerosis

ASJC Scopus subject areas

Immunology
Clinical Neurology
Immunology and Allergy
Neurology

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10.1016/j.jneuroim.2010.09.001

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Differences in exon expression and alternatively spliced genes in blood of multiple sclerosis compared to healthy control subjects. / Tian, Yingfang; Apperson, Michelle L ; Ander, Bradley ; Liu, Da; Stomova, Boryana S.; Jickling, Glen C.; Enriquez, Richelle; Agius, Mark A.; Sharp, Frank R.

In: Journal of Neuroimmunology, Vol. 230, No. 1-2, 01.2011, p. 124-129.

Research output: Contribution to journal › Article › peer-review

Tian, Yingfang ; Apperson, Michelle L ; Ander, Bradley ; Liu, Da ; Stomova, Boryana S. ; Jickling, Glen C. ; Enriquez, Richelle ; Agius, Mark A. ; Sharp, Frank R. / Differences in exon expression and alternatively spliced genes in blood of multiple sclerosis compared to healthy control subjects. In: Journal of Neuroimmunology. 2011 ; Vol. 230, No. 1-2. pp. 124-129.

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abstract = "Using whole genome exon microarrays 120 exons were differentially expressed between medication-free multiple sclerosis (MS) subjects in remission and healthy control subjects (HS) (p < 0.001, fold change > |1.2|). These exons differentiated MS from HS using cluster analyses, principal components analyses (PCAs) and cross-validation. In addition, 340 genes (transcripts) were predicted to be alternatively spliced in MS compared to HS. These findings may provide insight into the pathophysiology of MS and potentially provide prognostic and diagnostic biomarkers. However, given that multiple comparisons were performed on a very small sample, these preliminary findings require confirmation using a much larger independent cohort.",

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