Differences in Ca2+-handling and sarcoplasmic reticulum Ca2+-content in isolated rat and rabbit myocardium

Lars S. Maier, Donald M Bers, Burkert Pieske

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

We made novel measurements of the influence of rest intervals and stimulation frequency on twitch contractions and on sarcoplasmic reticulum (SR) Ca2--content (using rapid cooling contractures, RCCs) in isolated ventricular muscle strips from rat and rabbit hearts at a physiological temperature of 37 °C. In addition, the frequency-dependent relative contribution of SR Ca2+-uptake and Na+/Ca2+-exchange for cytosolic Ca2+-removal was assessed by paired RCCs. With increasing rest intervals (1-240 s) post-rest twitch force and RCC amplitude decreased monotonically in rabbit myocardium (after 240 s by 45 ± 10% and 61 ± 11%, respectively: P<0.05, n = 14). In contrast, rat myocardium (n = 11) exhibited a parallel increase in post-rest twitch force (by 67 ± 16% at 240 s; P<0.05) and RCC amplitude (by 20 ± 14%: P<0.05). In rabbit myocardium (n=11), increasing stimulation frequency from 0.25 to 3 Hz increased twitch force by 295 ± 50% (P<0.05) and RCC amplitude by 305 ± 80% (P<0.05). In contrast, in rat myocardium (n = 6), twitch force declined by 43 ± 7% (P<0.05), while RCC amplitude decreased only insignificantly (by 16 ± 7%). The SR Ca2+-uptake relative to Na+/Ca2+-exchange (based on paired RCCs) increased progressively with frequency in rabbit, but not in rat myocardium (∼66 ± 2% at all frequencies). We conclude that increased SR Ca2+-load contributes to the positive force-frequency relationship in rabbits and post-rest potentiation of twitch force in rats. Decreased SR Ca2+-load contributes to post-rest decay of twitch force in rabbits, but may play only a minor role in the negative force-frequency relationship in rats. SR Ca2+-release channel refractoriness may contribute importantly to the negative force-frequency relationship in rat and recovery from refractoriness may contribute to post-rest potentiation.

Original languageEnglish (US)
Pages (from-to)2249-2258
Number of pages10
JournalJournal of Molecular and Cellular Cardiology
Volume32
Issue number12
DOIs
StatePublished - 2000
Externally publishedYes

Fingerprint

Sarcoplasmic Reticulum
Contracture
Myocardium
Rabbits
Muscles
Temperature

Keywords

  • Force-frequency relationship
  • Post-rest behavior
  • Rapid cooling contractures
  • SR Ca-content

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this

Differences in Ca2+-handling and sarcoplasmic reticulum Ca2+-content in isolated rat and rabbit myocardium. / Maier, Lars S.; Bers, Donald M; Pieske, Burkert.

In: Journal of Molecular and Cellular Cardiology, Vol. 32, No. 12, 2000, p. 2249-2258.

Research output: Contribution to journalArticle

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abstract = "We made novel measurements of the influence of rest intervals and stimulation frequency on twitch contractions and on sarcoplasmic reticulum (SR) Ca2--content (using rapid cooling contractures, RCCs) in isolated ventricular muscle strips from rat and rabbit hearts at a physiological temperature of 37 °C. In addition, the frequency-dependent relative contribution of SR Ca2+-uptake and Na+/Ca2+-exchange for cytosolic Ca2+-removal was assessed by paired RCCs. With increasing rest intervals (1-240 s) post-rest twitch force and RCC amplitude decreased monotonically in rabbit myocardium (after 240 s by 45 ± 10{\%} and 61 ± 11{\%}, respectively: P<0.05, n = 14). In contrast, rat myocardium (n = 11) exhibited a parallel increase in post-rest twitch force (by 67 ± 16{\%} at 240 s; P<0.05) and RCC amplitude (by 20 ± 14{\%}: P<0.05). In rabbit myocardium (n=11), increasing stimulation frequency from 0.25 to 3 Hz increased twitch force by 295 ± 50{\%} (P<0.05) and RCC amplitude by 305 ± 80{\%} (P<0.05). In contrast, in rat myocardium (n = 6), twitch force declined by 43 ± 7{\%} (P<0.05), while RCC amplitude decreased only insignificantly (by 16 ± 7{\%}). The SR Ca2+-uptake relative to Na+/Ca2+-exchange (based on paired RCCs) increased progressively with frequency in rabbit, but not in rat myocardium (∼66 ± 2{\%} at all frequencies). We conclude that increased SR Ca2+-load contributes to the positive force-frequency relationship in rabbits and post-rest potentiation of twitch force in rats. Decreased SR Ca2+-load contributes to post-rest decay of twitch force in rabbits, but may play only a minor role in the negative force-frequency relationship in rats. SR Ca2+-release channel refractoriness may contribute importantly to the negative force-frequency relationship in rat and recovery from refractoriness may contribute to post-rest potentiation.",
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AB - We made novel measurements of the influence of rest intervals and stimulation frequency on twitch contractions and on sarcoplasmic reticulum (SR) Ca2--content (using rapid cooling contractures, RCCs) in isolated ventricular muscle strips from rat and rabbit hearts at a physiological temperature of 37 °C. In addition, the frequency-dependent relative contribution of SR Ca2+-uptake and Na+/Ca2+-exchange for cytosolic Ca2+-removal was assessed by paired RCCs. With increasing rest intervals (1-240 s) post-rest twitch force and RCC amplitude decreased monotonically in rabbit myocardium (after 240 s by 45 ± 10% and 61 ± 11%, respectively: P<0.05, n = 14). In contrast, rat myocardium (n = 11) exhibited a parallel increase in post-rest twitch force (by 67 ± 16% at 240 s; P<0.05) and RCC amplitude (by 20 ± 14%: P<0.05). In rabbit myocardium (n=11), increasing stimulation frequency from 0.25 to 3 Hz increased twitch force by 295 ± 50% (P<0.05) and RCC amplitude by 305 ± 80% (P<0.05). In contrast, in rat myocardium (n = 6), twitch force declined by 43 ± 7% (P<0.05), while RCC amplitude decreased only insignificantly (by 16 ± 7%). The SR Ca2+-uptake relative to Na+/Ca2+-exchange (based on paired RCCs) increased progressively with frequency in rabbit, but not in rat myocardium (∼66 ± 2% at all frequencies). We conclude that increased SR Ca2+-load contributes to the positive force-frequency relationship in rabbits and post-rest potentiation of twitch force in rats. Decreased SR Ca2+-load contributes to post-rest decay of twitch force in rabbits, but may play only a minor role in the negative force-frequency relationship in rats. SR Ca2+-release channel refractoriness may contribute importantly to the negative force-frequency relationship in rat and recovery from refractoriness may contribute to post-rest potentiation.

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