Dietary vitamin E in an atherogenic hamster model

Dietrich Rein, Wallace H. Yokoyama, Ruping Xu, Rosemary L. Walzem, J. Bruce German

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Regular consumption of fruits, vegetables and cereals moderates development of coronary heart disease and atherosclerosis in humans, possibly as a result of antioxidants present in these foods. The aim of this study was to develop a hamster model sufficiently sensitive to test the effects of nutritional rather than pharmacological amounts of food components on the development of atherosclerosis. The effect of vitamin E on aortic lipid deposition was investigated in hamsters fed a hypercholesterolemic diet containing per kilogram 2 g cholesterol, 90 g butterfat, 35 g vitamin E- stripped corn oil, 25 g fish oil and either the minimum requirement of 3 international units (IU) vitamin E or 30 IU. After 30 weeks, lipoprotein cholesterol fractions did not differ between groups, and the 6:1 LDL- cholesterol: HDL-cholesterol ratio was atherogenic. Early atherosclerosis was measured by lipid staining of aortic arch sections with oil red O and quantified by a photomicroscopy color-scanning technique. The area of lipid deposits in the 30 IU vitamin E group (7.9 ± 1.3%, mean ± SEM) was 58% less than in the 3 IU vitamin E group (18.7 ± 4.4%, p < 0.03). Hamsters fed a mixture of saturated and n-6 and n-3 polyunsaturated fatty acids plus cholesterol became hypercholesterolemic and were sensitive to vitamin E with respect to development of atherosclerosis. This model provides a tool to test atheroprotective effects of individual food components in vivo.

Original languageEnglish (US)
Pages (from-to)567-579
Number of pages13
JournalNutrition Research
Volume18
Issue number3
DOIs
StatePublished - Mar 1998

Fingerprint

Vitamin E
Cricetinae
Atherosclerosis
Lipids
Food
Cholesterol
Corn Oil
Fish Oils
Omega-3 Fatty Acids
Thoracic Aorta
Vegetables
LDL Cholesterol
HDL Cholesterol
Coronary Disease
Coronary Artery Disease
Fruit
Color
Antioxidants
Pharmacology
Staining and Labeling

Keywords

  • Aortic lipid deposits
  • Atherosclerosis
  • Dietary vitamin E
  • Hamsters
  • Plasma lipoprotein cholesterol

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Rein, D., Yokoyama, W. H., Xu, R., Walzem, R. L., & German, J. B. (1998). Dietary vitamin E in an atherogenic hamster model. Nutrition Research, 18(3), 567-579. https://doi.org/10.1016/S0271-5317(98)00043-8

Dietary vitamin E in an atherogenic hamster model. / Rein, Dietrich; Yokoyama, Wallace H.; Xu, Ruping; Walzem, Rosemary L.; German, J. Bruce.

In: Nutrition Research, Vol. 18, No. 3, 03.1998, p. 567-579.

Research output: Contribution to journalArticle

Rein, D, Yokoyama, WH, Xu, R, Walzem, RL & German, JB 1998, 'Dietary vitamin E in an atherogenic hamster model', Nutrition Research, vol. 18, no. 3, pp. 567-579. https://doi.org/10.1016/S0271-5317(98)00043-8
Rein D, Yokoyama WH, Xu R, Walzem RL, German JB. Dietary vitamin E in an atherogenic hamster model. Nutrition Research. 1998 Mar;18(3):567-579. https://doi.org/10.1016/S0271-5317(98)00043-8
Rein, Dietrich ; Yokoyama, Wallace H. ; Xu, Ruping ; Walzem, Rosemary L. ; German, J. Bruce. / Dietary vitamin E in an atherogenic hamster model. In: Nutrition Research. 1998 ; Vol. 18, No. 3. pp. 567-579.
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