Dietary vitamin E and pulmonary biochemical and morphological alterations of rats exposed to 0.1 ppm ozone

C. K. Chow, Charles Plopper, M. Chiu, D. L. Dungworth

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Three groups of 28 1-month-old male Sprague-Dawley rats each were fed a basal vitamin E-deficient diet and supplemented with either 0, 11, or 110 ppm vitamin E for 38 days, and were then exposed to 0 or 0.1 ppm ozone continuously for 7 days. Following ozone exposure, the level of reduced glutathione (GSH) and activities of GSH peroxidase, GSH reductase, glucose-6-phosphate dehydrogenase (G-6-PD), and lactate dehydrogenase (LDH), but not of malic dehydrogenase, were significantly elevated in the lungs of rats fed the vitamin E deficient diet. The level of GSH and activities of GSH peroxidase and G-6-PD were also significantly increased in the lungs of the animal group fed the 11 ppm vitamin E diet, while none of the biochemical measurements made was significantly altered by ozone in the 110-ppm vitamin E diet fed rats. Scanning electron microscope examination revealed that five out of six rats on the vitamin E-deficient diet and four out of six from the 11-ppm vitamin E diet had detectable lesions following ozone exposure, as compared with only one of the six exposed animals from the 110-ppm vitamin E diet. The lesion was restricted to bronchiolar epithelium and alveoli immediately adjacent to the bronchiole-alveolar duct junction. None of the control animals had detectable lesions. The results suggest that exposure to ozone at 0.1-ppm level can produce detectable pulmonary damage, and that dietary vitamin E alters pulmonary susceptibility to ozone exposure.

Original languageEnglish (US)
Pages (from-to)315-324
Number of pages10
JournalEnvironmental Research
Volume24
Issue number2
DOIs
StatePublished - Jan 1 1981

ASJC Scopus subject areas

  • Biochemistry
  • Environmental Science(all)

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