Dietary long-chain omega-3 fatty acids do not diminish eosinophilic pulmonary inflammation in mice

Gertrud U. Schuster, Jennifer M. Bratt, Xiaowen Jiang, Theresa L. Pedersen, Dmitry Grapov, Yuriko Adkins, Darshan S. Kelley, John W. Newman, Nicholas Kenyon, Charles B. Stephensen

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Although the effects of fish oil supplements on airway inflammation in asthma have been studied with varying results, the independent effects of the fish oil components, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), administered separately, are untested. Here, we investigated airway inflammation and hyperresponsiveness using a mouse ovalbumin exposure model of asthma assessing the effects of consuming EPA (1.5% wt/wt), DHA (1.5% wt/wt), EPA plus DHA (0.75% each), or a control diet with no added omega-3 polyunsaturated fatty acids. Consuming these diets for ± weeks resulted in erythrocyte membrane EPA contents (molar %) of 9.0 (±0.6), 3.2 (±0.2), 6.8 (±0.5), and 0.01 (±0.0)%; DHA contents were 6.8 (±0.1), 15.6 (±0.5), 12.3 (±0.3), and 3.8 (±0.2)%, respectively. The DHA group had the highest bronchoalveolar lavage (BAL) fluid eosinophil and IL-6 levels (P < 0.05). Similar trends were seen for macrophages, IL-4, and IL-13, whereas TNF-α was lower in omega-3 polyunsaturated fatty acid groups than the control (P < 0.05). The DHA group also had the highest airway resistance, which differed significantly from the EPA plus DHA group (P < 0.05), which had the lowest. Oxylipins were measured in plasma and BAL fluid, with DHA and EPA suppressing arachidonic acid-derived oxylipin production. DHA-derived oxylipins from the cytochrome P450 and 15-lipoxygenase pathways correlated significantly with BAL eosinophil levels. The proinflammatory effects of DHA suggest that the adverse effects of individual fatty acid formulations should be thoroughly considered before any use as therapeutic agents in asthma.

Original languageEnglish (US)
Pages (from-to)626-636
Number of pages11
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume50
Issue number3
DOIs
StatePublished - 2014

Fingerprint

Docosahexaenoic Acids
Omega-3 Fatty Acids
Pneumonia
Eicosapentaenoic Acid
Oxylipins
Asthma
Fish Oils
Bronchoalveolar Lavage Fluid
Nutrition
Unsaturated Fatty Acids
Eosinophils
Arachidonate 15-Lipoxygenase
Diet
Inflammation
Airway Resistance
Fluids
Interleukin-13
Macrophages
Ovalbumin
Erythrocyte Membrane

Keywords

  • Eosinophils
  • Inflammation
  • Lipid mediators
  • Rodents

ASJC Scopus subject areas

  • Cell Biology
  • Pulmonary and Respiratory Medicine
  • Molecular Biology
  • Clinical Biochemistry

Cite this

Schuster, G. U., Bratt, J. M., Jiang, X., Pedersen, T. L., Grapov, D., Adkins, Y., ... Stephensen, C. B. (2014). Dietary long-chain omega-3 fatty acids do not diminish eosinophilic pulmonary inflammation in mice. American Journal of Respiratory Cell and Molecular Biology, 50(3), 626-636. https://doi.org/10.1165/rcmb.2013-0136OC

Dietary long-chain omega-3 fatty acids do not diminish eosinophilic pulmonary inflammation in mice. / Schuster, Gertrud U.; Bratt, Jennifer M.; Jiang, Xiaowen; Pedersen, Theresa L.; Grapov, Dmitry; Adkins, Yuriko; Kelley, Darshan S.; Newman, John W.; Kenyon, Nicholas; Stephensen, Charles B.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 50, No. 3, 2014, p. 626-636.

Research output: Contribution to journalArticle

Schuster, GU, Bratt, JM, Jiang, X, Pedersen, TL, Grapov, D, Adkins, Y, Kelley, DS, Newman, JW, Kenyon, N & Stephensen, CB 2014, 'Dietary long-chain omega-3 fatty acids do not diminish eosinophilic pulmonary inflammation in mice', American Journal of Respiratory Cell and Molecular Biology, vol. 50, no. 3, pp. 626-636. https://doi.org/10.1165/rcmb.2013-0136OC
Schuster, Gertrud U. ; Bratt, Jennifer M. ; Jiang, Xiaowen ; Pedersen, Theresa L. ; Grapov, Dmitry ; Adkins, Yuriko ; Kelley, Darshan S. ; Newman, John W. ; Kenyon, Nicholas ; Stephensen, Charles B. / Dietary long-chain omega-3 fatty acids do not diminish eosinophilic pulmonary inflammation in mice. In: American Journal of Respiratory Cell and Molecular Biology. 2014 ; Vol. 50, No. 3. pp. 626-636.
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