Dietary fat and aging modulate apoptotic signaling in liver of calorie-restricted mice

José Alberto López-Domínguez, Husam Khraiwesh, José Antonio González-Reyes, Guillermo López-Lluch, Plácido Navas, Jon J Ramsey, Rafael De Cabo, María Isabel Burón, José Manuel Villalba

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Imbalance between proliferation and cell death accounts for several age-linked diseases. Aging, calorie restriction (CR), and fat source are all factors that may influence apoptotic signaling in liver, an organ that plays a central metabolic role in the organism. Here, we have studied the combined effect of these factors on a number of apoptosis regulators and effectors. For this purpose, animals were fed diets containing different fat sources (lard, soybean oil, or fish oil) under CR for 6 or 18 months. An age-linked increase in the mitochondrial apoptotic pathway was detected with CR, including a decrease in Bcl-2/Bax ratio, an enhanced release of cytochrome c to the cytosol and higher caspase-9 activity. However, these changes were not fully transmitted to the effectors apoptosis-inducing factor and caspase-3. CR (which abated aging-related inflammatory responses) and dietary fat altered the activities of caspases-8, -9, and -3. Apoptotic index (DNA fragmentation) and mean nuclear area were increased in aged animals with the exception of calorie-restricted mice fed a lard-based fat source. These results suggest possible protective changes in hepatic homeostasis with aging in the calorie-restricted lard group.

Original languageEnglish (US)
Pages (from-to)399-409
Number of pages11
JournalJournals of Gerontology - Series A Biological Sciences and Medical Sciences
Volume70
Issue number4
DOIs
StatePublished - Apr 1 2015

Fingerprint

Dietary Fats
Caspase 9
Fats
Liver
Apoptosis Inducing Factor
Soybean Oil
Caspase 8
Fish Oils
DNA Fragmentation
Cytochromes c
Caspase 3
Cytosol
Homeostasis
Cell Death
Apoptosis
Diet
lard

Keywords

  • Apoptosis
  • Calorie restriction
  • Dietary fat-Liver

ASJC Scopus subject areas

  • Aging
  • Geriatrics and Gerontology

Cite this

López-Domínguez, J. A., Khraiwesh, H., González-Reyes, J. A., López-Lluch, G., Navas, P., Ramsey, J. J., ... Villalba, J. M. (2015). Dietary fat and aging modulate apoptotic signaling in liver of calorie-restricted mice. Journals of Gerontology - Series A Biological Sciences and Medical Sciences, 70(4), 399-409. https://doi.org/10.1093/gerona/glu045

Dietary fat and aging modulate apoptotic signaling in liver of calorie-restricted mice. / López-Domínguez, José Alberto; Khraiwesh, Husam; González-Reyes, José Antonio; López-Lluch, Guillermo; Navas, Plácido; Ramsey, Jon J; De Cabo, Rafael; Burón, María Isabel; Villalba, José Manuel.

In: Journals of Gerontology - Series A Biological Sciences and Medical Sciences, Vol. 70, No. 4, 01.04.2015, p. 399-409.

Research output: Contribution to journalArticle

López-Domínguez, JA, Khraiwesh, H, González-Reyes, JA, López-Lluch, G, Navas, P, Ramsey, JJ, De Cabo, R, Burón, MI & Villalba, JM 2015, 'Dietary fat and aging modulate apoptotic signaling in liver of calorie-restricted mice', Journals of Gerontology - Series A Biological Sciences and Medical Sciences, vol. 70, no. 4, pp. 399-409. https://doi.org/10.1093/gerona/glu045
López-Domínguez, José Alberto ; Khraiwesh, Husam ; González-Reyes, José Antonio ; López-Lluch, Guillermo ; Navas, Plácido ; Ramsey, Jon J ; De Cabo, Rafael ; Burón, María Isabel ; Villalba, José Manuel. / Dietary fat and aging modulate apoptotic signaling in liver of calorie-restricted mice. In: Journals of Gerontology - Series A Biological Sciences and Medical Sciences. 2015 ; Vol. 70, No. 4. pp. 399-409.
@article{7a5bc46d7e9f4ca8871aaa58bd492aa5,
title = "Dietary fat and aging modulate apoptotic signaling in liver of calorie-restricted mice",
abstract = "Imbalance between proliferation and cell death accounts for several age-linked diseases. Aging, calorie restriction (CR), and fat source are all factors that may influence apoptotic signaling in liver, an organ that plays a central metabolic role in the organism. Here, we have studied the combined effect of these factors on a number of apoptosis regulators and effectors. For this purpose, animals were fed diets containing different fat sources (lard, soybean oil, or fish oil) under CR for 6 or 18 months. An age-linked increase in the mitochondrial apoptotic pathway was detected with CR, including a decrease in Bcl-2/Bax ratio, an enhanced release of cytochrome c to the cytosol and higher caspase-9 activity. However, these changes were not fully transmitted to the effectors apoptosis-inducing factor and caspase-3. CR (which abated aging-related inflammatory responses) and dietary fat altered the activities of caspases-8, -9, and -3. Apoptotic index (DNA fragmentation) and mean nuclear area were increased in aged animals with the exception of calorie-restricted mice fed a lard-based fat source. These results suggest possible protective changes in hepatic homeostasis with aging in the calorie-restricted lard group.",
keywords = "Apoptosis, Calorie restriction, Dietary fat-Liver",
author = "L{\'o}pez-Dom{\'i}nguez, {Jos{\'e} Alberto} and Husam Khraiwesh and Gonz{\'a}lez-Reyes, {Jos{\'e} Antonio} and Guillermo L{\'o}pez-Lluch and Pl{\'a}cido Navas and Ramsey, {Jon J} and {De Cabo}, Rafael and Bur{\'o}n, {Mar{\'i}a Isabel} and Villalba, {Jos{\'e} Manuel}",
year = "2015",
month = "4",
day = "1",
doi = "10.1093/gerona/glu045",
language = "English (US)",
volume = "70",
pages = "399--409",
journal = "The journals of gerontology. Series A, Biological sciences and medical sciences",
issn = "1079-5006",
publisher = "Oxford University Press",
number = "4",

}

TY - JOUR

T1 - Dietary fat and aging modulate apoptotic signaling in liver of calorie-restricted mice

AU - López-Domínguez, José Alberto

AU - Khraiwesh, Husam

AU - González-Reyes, José Antonio

AU - López-Lluch, Guillermo

AU - Navas, Plácido

AU - Ramsey, Jon J

AU - De Cabo, Rafael

AU - Burón, María Isabel

AU - Villalba, José Manuel

PY - 2015/4/1

Y1 - 2015/4/1

N2 - Imbalance between proliferation and cell death accounts for several age-linked diseases. Aging, calorie restriction (CR), and fat source are all factors that may influence apoptotic signaling in liver, an organ that plays a central metabolic role in the organism. Here, we have studied the combined effect of these factors on a number of apoptosis regulators and effectors. For this purpose, animals were fed diets containing different fat sources (lard, soybean oil, or fish oil) under CR for 6 or 18 months. An age-linked increase in the mitochondrial apoptotic pathway was detected with CR, including a decrease in Bcl-2/Bax ratio, an enhanced release of cytochrome c to the cytosol and higher caspase-9 activity. However, these changes were not fully transmitted to the effectors apoptosis-inducing factor and caspase-3. CR (which abated aging-related inflammatory responses) and dietary fat altered the activities of caspases-8, -9, and -3. Apoptotic index (DNA fragmentation) and mean nuclear area were increased in aged animals with the exception of calorie-restricted mice fed a lard-based fat source. These results suggest possible protective changes in hepatic homeostasis with aging in the calorie-restricted lard group.

AB - Imbalance between proliferation and cell death accounts for several age-linked diseases. Aging, calorie restriction (CR), and fat source are all factors that may influence apoptotic signaling in liver, an organ that plays a central metabolic role in the organism. Here, we have studied the combined effect of these factors on a number of apoptosis regulators and effectors. For this purpose, animals were fed diets containing different fat sources (lard, soybean oil, or fish oil) under CR for 6 or 18 months. An age-linked increase in the mitochondrial apoptotic pathway was detected with CR, including a decrease in Bcl-2/Bax ratio, an enhanced release of cytochrome c to the cytosol and higher caspase-9 activity. However, these changes were not fully transmitted to the effectors apoptosis-inducing factor and caspase-3. CR (which abated aging-related inflammatory responses) and dietary fat altered the activities of caspases-8, -9, and -3. Apoptotic index (DNA fragmentation) and mean nuclear area were increased in aged animals with the exception of calorie-restricted mice fed a lard-based fat source. These results suggest possible protective changes in hepatic homeostasis with aging in the calorie-restricted lard group.

KW - Apoptosis

KW - Calorie restriction

KW - Dietary fat-Liver

UR - http://www.scopus.com/inward/record.url?scp=84926609154&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84926609154&partnerID=8YFLogxK

U2 - 10.1093/gerona/glu045

DO - 10.1093/gerona/glu045

M3 - Article

C2 - 24691092

AN - SCOPUS:84926609154

VL - 70

SP - 399

EP - 409

JO - The journals of gerontology. Series A, Biological sciences and medical sciences

JF - The journals of gerontology. Series A, Biological sciences and medical sciences

SN - 1079-5006

IS - 4

ER -