Diet-induced obesity leads to the development of leptin resistance in vagal afferent neurons

Guillaume de Lartigue, Claire Barbier de la Serre, Elvis Espero, Jennifer Lee, Helen E Raybould

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

Ingestion of high-fat, high-calorie diets is associated with hyperphagia, increased body fat, and obesity. The mechanisms responsible are currently unclear; however, altered leptin signaling may be an important factor. Vagal afferent neurons (VAN) integrate signals from the gut in response to ingestion of nutrients and express leptin receptors. Therefore, we tested the hypothesis that leptin resistance occurs in VAN in response to a high-fat diet. Sprague-Dawley rats, which exhibit a bimodal distribution of body weight gain, were used after ingestion of a high-fat diet for 8 wk. Body weight, food intake, and plasma leptin levels were measured. Leptin signaling was determined by immunohistochemical localization of phosphorylated STAT3 (pSTAT3) in cultured VAN and by quantifaction of pSTAT3 protein levels by Western blot analysis in nodose ganglia and arcuate nucleus in vivo. To determine the mechanism of leptin resistance in nodose ganglia, cultured VAN were stimulated with leptin alone or with lipopolysaccharide (LPS) and SOCS-3 expression measured. SOCS-3 protein levels in VAN were measured by Western blot following leptin administration in vivo. Leptin resulted in appearance of pSTAT3 in VAN of low-fat-fed rats and rats resistant to diet-induced obesity but not diet-induced obese (DIO) rats. However, leptin signaling was normal in arcuate neurons. SOCS-3 expression was increased in VAN of DIO rats. In cultured VAN, LPS increased SOCS-3 expression and inhibited leptin-induced pSTAT3 in vivo. We conclude that VAN of diet-induced obese rats become leptin resistant; LPS and SOCS-3 may play a role in the development of leptin resistance.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume301
Issue number1
DOIs
StatePublished - Jul 2011

Fingerprint

Afferent Neurons
Leptin
Obesity
Diet
High Fat Diet
Eating
Nodose Ganglion
Lipopolysaccharides
Suppressor of Cytokine Signaling Proteins
Western Blotting
Body Weight
Leptin Receptors
STAT3 Transcription Factor
Hyperphagia
Arcuate Nucleus of Hypothalamus
Weight Gain
Sprague Dawley Rats
Adipose Tissue

Keywords

  • High-fat diet
  • Lipopolysaccharide
  • Suppressor of cytokine signaling-3

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)
  • Endocrinology, Diabetes and Metabolism

Cite this

Diet-induced obesity leads to the development of leptin resistance in vagal afferent neurons. / de Lartigue, Guillaume; de la Serre, Claire Barbier; Espero, Elvis; Lee, Jennifer; Raybould, Helen E.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 301, No. 1, 07.2011.

Research output: Contribution to journalArticle

de Lartigue, Guillaume ; de la Serre, Claire Barbier ; Espero, Elvis ; Lee, Jennifer ; Raybould, Helen E. / Diet-induced obesity leads to the development of leptin resistance in vagal afferent neurons. In: American Journal of Physiology - Endocrinology and Metabolism. 2011 ; Vol. 301, No. 1.
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