Abstract
Objective-To determine diagnostic accuracy of using erythrocyte indices and polychromasia to identify regenerative anemia in dogs. Design-Retrospective and prospective cross-sectional study. Animals-4,521 anemic dogs. Procedures-CBC results obtained between July 2002 and July 2008 by use of an automated laser-based flow cytometric hematology analyzer from dogs with Hct values ≤ 35% were retrieved. Sensitivity, specificity, accuracy, and predictive values of using erythrocyte indices and polychromasia to identify regeneration were determined, with a reticulocyte count > 65,000 reticulocytes/μL considered the gold standard. Similarly, 134 blood samples from anemic dogs were analyzed prospectively with an in-house electrical impedance analyzer. Results-Of 4,387 dogs with samples analyzed retrospectively, 1,426 (32.5%) had regenerative anemia. Of these, 168 (11.8%) had macrocytic hypochromic anemia. High mean cell volume and low mean cell hemoglobin concentration had low sensitivity (11%), high specificity (98%), and moderate accuracy (70%) when used to identify regenerative anemia. Use of polychromasia alone had an accuracy of 77%, and use of polychromasia combined with a high RBC distribution width (RDW) had an accuracy of 79%. Results obtained with the in-house analyzer were similar. Conclusions and Clinical Relevance-Results suggested that most regenerative anemias in dogs were not macrocytic hypochromic. Polychromasia, with or without high RDW, was a more accurate indicator than other erythrocyte indices of regenerative anemia. To avoid a false diagnosis of nonregenerative anemia, a blood smear should be evaluated in anemic dogs when a reticulocyte count is not available.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1452-1458 |
| Number of pages | 7 |
| Journal | Journal of the American Veterinary Medical Association |
| Volume | 238 |
| Issue number | 11 |
| DOIs | |
| State | Published - Jun 1 2011 |
Fingerprint
ASJC Scopus subject areas
- veterinary(all)
Cite this
Diagnostic accuracy of using erythrocyte indices and polychromasia to identify regenerative anemia in dogs. / Hodges, Joanne; Christopher, Mary M.
In: Journal of the American Veterinary Medical Association, Vol. 238, No. 11, 01.06.2011, p. 1452-1458.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Diagnostic accuracy of using erythrocyte indices and polychromasia to identify regenerative anemia in dogs
AU - Hodges, Joanne
AU - Christopher, Mary M
PY - 2011/6/1
Y1 - 2011/6/1
N2 - Objective-To determine diagnostic accuracy of using erythrocyte indices and polychromasia to identify regenerative anemia in dogs. Design-Retrospective and prospective cross-sectional study. Animals-4,521 anemic dogs. Procedures-CBC results obtained between July 2002 and July 2008 by use of an automated laser-based flow cytometric hematology analyzer from dogs with Hct values ≤ 35% were retrieved. Sensitivity, specificity, accuracy, and predictive values of using erythrocyte indices and polychromasia to identify regeneration were determined, with a reticulocyte count > 65,000 reticulocytes/μL considered the gold standard. Similarly, 134 blood samples from anemic dogs were analyzed prospectively with an in-house electrical impedance analyzer. Results-Of 4,387 dogs with samples analyzed retrospectively, 1,426 (32.5%) had regenerative anemia. Of these, 168 (11.8%) had macrocytic hypochromic anemia. High mean cell volume and low mean cell hemoglobin concentration had low sensitivity (11%), high specificity (98%), and moderate accuracy (70%) when used to identify regenerative anemia. Use of polychromasia alone had an accuracy of 77%, and use of polychromasia combined with a high RBC distribution width (RDW) had an accuracy of 79%. Results obtained with the in-house analyzer were similar. Conclusions and Clinical Relevance-Results suggested that most regenerative anemias in dogs were not macrocytic hypochromic. Polychromasia, with or without high RDW, was a more accurate indicator than other erythrocyte indices of regenerative anemia. To avoid a false diagnosis of nonregenerative anemia, a blood smear should be evaluated in anemic dogs when a reticulocyte count is not available.
AB - Objective-To determine diagnostic accuracy of using erythrocyte indices and polychromasia to identify regenerative anemia in dogs. Design-Retrospective and prospective cross-sectional study. Animals-4,521 anemic dogs. Procedures-CBC results obtained between July 2002 and July 2008 by use of an automated laser-based flow cytometric hematology analyzer from dogs with Hct values ≤ 35% were retrieved. Sensitivity, specificity, accuracy, and predictive values of using erythrocyte indices and polychromasia to identify regeneration were determined, with a reticulocyte count > 65,000 reticulocytes/μL considered the gold standard. Similarly, 134 blood samples from anemic dogs were analyzed prospectively with an in-house electrical impedance analyzer. Results-Of 4,387 dogs with samples analyzed retrospectively, 1,426 (32.5%) had regenerative anemia. Of these, 168 (11.8%) had macrocytic hypochromic anemia. High mean cell volume and low mean cell hemoglobin concentration had low sensitivity (11%), high specificity (98%), and moderate accuracy (70%) when used to identify regenerative anemia. Use of polychromasia alone had an accuracy of 77%, and use of polychromasia combined with a high RBC distribution width (RDW) had an accuracy of 79%. Results obtained with the in-house analyzer were similar. Conclusions and Clinical Relevance-Results suggested that most regenerative anemias in dogs were not macrocytic hypochromic. Polychromasia, with or without high RDW, was a more accurate indicator than other erythrocyte indices of regenerative anemia. To avoid a false diagnosis of nonregenerative anemia, a blood smear should be evaluated in anemic dogs when a reticulocyte count is not available.
UR - http://www.scopus.com/inward/record.url?scp=79958030667&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79958030667&partnerID=8YFLogxK
U2 - 10.2460/javma.238.11.1452
DO - 10.2460/javma.238.11.1452
M3 - Article
C2 - 21627508
AN - SCOPUS:79958030667
VL - 238
SP - 1452
EP - 1458
JO - Journal of the American Veterinary Medical Association
JF - Journal of the American Veterinary Medical Association
SN - 0003-1488
IS - 11
ER -