Diagnosis of Equine Protozoal Myeloencephalitis Using Indirect Fluorescent Antibody Testing and Enzyme-Linked Immunosorbent Assay Titer Ratios for Sarcocystis neurona and Neospora hughesi

Anna C. Renier, Jennifer K. Morrow, Amy J. Graves, Carrie J Finno, Daniel K. Howe, Sean D Owens, Eva Tamez-Trevino, Andrea E. Packham, Patricia A Conrad, Nicola Pusterla

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The aim of this study was to compare two serologic tests used to support a diagnosis of equine protozoal myeloencephalitis (EPM). Serum and cerebrospinal fluid (CSF) samples were analyzed for antibodies to Sarcocystis neurona and Neospora hughesi by indirect fluorescent antibody testing (IFAT) and surface antigens of S. neurona and N. hughesi by enzyme-linked immunosorbent assay (ELISA). The samples originated from neurologic horses with confirmed and suspected EPM (nine S. neurona, three N. hughesi), from neurologic horses with confirmed neurologic diseases other than EPM (16 horses) and from healthy horses (10). The IFAT on CSF and ELISA titer ratios showed equal sensitivity in diagnosing EPM caused by S. neurona. The ELISA titer ratios showed slightly greater specificity in diagnosing EPM than the IFAT on CSF. Overall agreement between the IFAT on CSF and ELISA titer ratio was 90.9%. The IFAT on CSF and ELISA serum/CSF ratio are indicated to help support a laboratory diagnosis of EPM.

Original languageEnglish (US)
Pages (from-to)49-51
Number of pages3
JournalJournal of Equine Veterinary Science
Volume36
DOIs
StatePublished - Jan 1 2016

Fingerprint

Neospora hughesi
equine protozoal myeloencephalitis
Sarcocystis
Neospora
Sarcocystis neurona
Encephalomyelitis
cerebrospinal fluid
antibody detection
Horses
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
Antibodies
Cerebrospinal Fluid
horses
nervous system
nervous system diseases
surface antigens
Nervous System
immunologic techniques
Clinical Laboratory Techniques

Keywords

  • ELISA
  • EPM
  • IFAT
  • Neospora hughesi
  • Sarcocystis neurona

ASJC Scopus subject areas

  • Equine

Cite this

Diagnosis of Equine Protozoal Myeloencephalitis Using Indirect Fluorescent Antibody Testing and Enzyme-Linked Immunosorbent Assay Titer Ratios for Sarcocystis neurona and Neospora hughesi. / Renier, Anna C.; Morrow, Jennifer K.; Graves, Amy J.; Finno, Carrie J; Howe, Daniel K.; Owens, Sean D; Tamez-Trevino, Eva; Packham, Andrea E.; Conrad, Patricia A; Pusterla, Nicola.

In: Journal of Equine Veterinary Science, Vol. 36, 01.01.2016, p. 49-51.

Research output: Contribution to journalArticle

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