Diagnosis of Equine Protozoal Myeloencephalitis Using Indirect Fluorescent Antibody Testing and Enzyme-Linked Immunosorbent Assay Titer Ratios for Sarcocystis neurona and Neospora hughesi

Anna C. Renier, Jennifer K. Morrow, Amy J. Graves, Carrie J Finno, Daniel K. Howe, Sean D Owens, Eva Tamez-Trevino, Andrea E. Packham, Patricia A Conrad, Nicola Pusterla

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2 Scopus citations

Abstract

The aim of this study was to compare two serologic tests used to support a diagnosis of equine protozoal myeloencephalitis (EPM). Serum and cerebrospinal fluid (CSF) samples were analyzed for antibodies to Sarcocystis neurona and Neospora hughesi by indirect fluorescent antibody testing (IFAT) and surface antigens of S. neurona and N. hughesi by enzyme-linked immunosorbent assay (ELISA). The samples originated from neurologic horses with confirmed and suspected EPM (nine S. neurona, three N. hughesi), from neurologic horses with confirmed neurologic diseases other than EPM (16 horses) and from healthy horses (10). The IFAT on CSF and ELISA titer ratios showed equal sensitivity in diagnosing EPM caused by S. neurona. The ELISA titer ratios showed slightly greater specificity in diagnosing EPM than the IFAT on CSF. Overall agreement between the IFAT on CSF and ELISA titer ratio was 90.9%. The IFAT on CSF and ELISA serum/CSF ratio are indicated to help support a laboratory diagnosis of EPM.

Original languageEnglish (US)
Pages (from-to)49-51
Number of pages3
JournalJournal of Equine Veterinary Science
Volume36
DOIs
StatePublished - Jan 1 2016

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Keywords

  • ELISA
  • EPM
  • IFAT
  • Neospora hughesi
  • Sarcocystis neurona

ASJC Scopus subject areas

  • Equine

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