Abstract
To assess the possibility of standardization of a commonly used indirect immunofluorescent antibody (IFA) test for detection of Babesia microti antibody in human sera, the results from four reference laboratories were compared. Patients with babesiosis from southern New England (n = 25) and subjects with no history ofbabesiosis from southern New England (n = 55) and Iceland (n = 50) were enrolled in the study. Anti-Babesia antibody titers were determined in a blinded fashion by IFA test. The range of test results in the four laboratories was 88%-96% sensitivity, 90%-100% specificity, 69%-100% positive predictive value, and 96%-99% negative predictive value. Interlaboratory and intralaboratory concordance ranged from 84% to 85% and 94% to 100%, respectively. This B. microti IFA procedure is a sensitive, specific, and reproducible method for diagnosing babesiosis and is suitable for use as a standard in laboratories testing human sera for B. microti antibody.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 916-923 |
| Number of pages | 8 |
| Journal | Journal of Infectious Diseases |
| Volume | 169 |
| Issue number | 4 |
| DOIs | |
| State | Published - 1994 |
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ASJC Scopus subject areas
- Infectious Diseases
- Immunology and Allergy
Cite this
Diagnosis of babesiosis : Evaluation of a serologic test for the detection of babesia microti antibody. / Krause, Peter J.; Telford, Sam R.; Ryan, Raymond; Conrad, Patricia A; Wilson, Marianna; Thomford, John W.; Spielman, Andrew.
In: Journal of Infectious Diseases, Vol. 169, No. 4, 1994, p. 916-923.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Diagnosis of babesiosis
T2 - Evaluation of a serologic test for the detection of babesia microti antibody
AU - Krause, Peter J.
AU - Telford, Sam R.
AU - Ryan, Raymond
AU - Conrad, Patricia A
AU - Wilson, Marianna
AU - Thomford, John W.
AU - Spielman, Andrew
PY - 1994
Y1 - 1994
N2 - To assess the possibility of standardization of a commonly used indirect immunofluorescent antibody (IFA) test for detection of Babesia microti antibody in human sera, the results from four reference laboratories were compared. Patients with babesiosis from southern New England (n = 25) and subjects with no history ofbabesiosis from southern New England (n = 55) and Iceland (n = 50) were enrolled in the study. Anti-Babesia antibody titers were determined in a blinded fashion by IFA test. The range of test results in the four laboratories was 88%-96% sensitivity, 90%-100% specificity, 69%-100% positive predictive value, and 96%-99% negative predictive value. Interlaboratory and intralaboratory concordance ranged from 84% to 85% and 94% to 100%, respectively. This B. microti IFA procedure is a sensitive, specific, and reproducible method for diagnosing babesiosis and is suitable for use as a standard in laboratories testing human sera for B. microti antibody.
AB - To assess the possibility of standardization of a commonly used indirect immunofluorescent antibody (IFA) test for detection of Babesia microti antibody in human sera, the results from four reference laboratories were compared. Patients with babesiosis from southern New England (n = 25) and subjects with no history ofbabesiosis from southern New England (n = 55) and Iceland (n = 50) were enrolled in the study. Anti-Babesia antibody titers were determined in a blinded fashion by IFA test. The range of test results in the four laboratories was 88%-96% sensitivity, 90%-100% specificity, 69%-100% positive predictive value, and 96%-99% negative predictive value. Interlaboratory and intralaboratory concordance ranged from 84% to 85% and 94% to 100%, respectively. This B. microti IFA procedure is a sensitive, specific, and reproducible method for diagnosing babesiosis and is suitable for use as a standard in laboratories testing human sera for B. microti antibody.
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U2 - 10.1093/infdis/169.4.923
DO - 10.1093/infdis/169.4.923
M3 - Article
VL - 169
SP - 916
EP - 923
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 4
ER -