Diagnosis of babesiosis: Evaluation of a serologic test for the detection of babesia microti antibody

Peter J. Krause, Sam R. Telford, Raymond Ryan, Patricia A Conrad, Marianna Wilson, John W. Thomford, Andrew Spielman

Research output: Contribution to journalArticlepeer-review

118 Scopus citations


To assess the possibility of standardization of a commonly used indirect immunofluorescent antibody (IFA) test for detection of Babesia microti antibody in human sera, the results from four reference laboratories were compared. Patients with babesiosis from southern New England (n = 25) and subjects with no history ofbabesiosis from southern New England (n = 55) and Iceland (n = 50) were enrolled in the study. Anti-Babesia antibody titers were determined in a blinded fashion by IFA test. The range of test results in the four laboratories was 88%-96% sensitivity, 90%-100% specificity, 69%-100% positive predictive value, and 96%-99% negative predictive value. Interlaboratory and intralaboratory concordance ranged from 84% to 85% and 94% to 100%, respectively. This B. microti IFA procedure is a sensitive, specific, and reproducible method for diagnosing babesiosis and is suitable for use as a standard in laboratories testing human sera for B. microti antibody.

Original languageEnglish (US)
Pages (from-to)916-923
Number of pages8
JournalJournal of Infectious Diseases
Issue number4
StatePublished - 1994

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy


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