Abstract
Glucocorticoids specifically increase Kv1.5 K+ channel mRNA in normal and clonal (GH3) rat pituitary cells. Here, we demonstrate that dexamethasone, a glucocorticoid agonist, rapidly induces Kv1.5 gene transcription, but does not affect Kv1.5 mRNA turnover t 1 2 ≈ 0.5 hr) in GH3 cells. Immunoblots indicate that the steroid also increases the expression of the 76 kd Kv1.5 protein 3-fold within 12 hr without altering its half-life (t 1 2 ≈ 4 hr). In contrast, Kv1.4 protein expression is unaffected. Finally, we find that the induction of Kv1.5 protein is associated with an increase in a noninactivating component of the voltage-gated K+ current. Our results indicate that hormones and neurotransmitters may act within hours to regulate excitability by controlling K+ channel gene expression.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 359-369 |
| Number of pages | 11 |
| Journal | Neuron |
| Volume | 11 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1993 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Neuroscience(all)
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Dexamethasone rapidly induces Kv1.5 K+ channel gene transcription and expression in clonal pituitary cells. / Takimoto, Koichi; Fomina, Alla F; Gealy, Robert; Trimmer, James; Levitan, Edwin S.
In: Neuron, Vol. 11, No. 2, 1993, p. 359-369.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Dexamethasone rapidly induces Kv1.5 K+ channel gene transcription and expression in clonal pituitary cells
AU - Takimoto, Koichi
AU - Fomina, Alla F
AU - Gealy, Robert
AU - Trimmer, James
AU - Levitan, Edwin S.
PY - 1993
Y1 - 1993
N2 - Glucocorticoids specifically increase Kv1.5 K+ channel mRNA in normal and clonal (GH3) rat pituitary cells. Here, we demonstrate that dexamethasone, a glucocorticoid agonist, rapidly induces Kv1.5 gene transcription, but does not affect Kv1.5 mRNA turnover t 1 2 ≈ 0.5 hr) in GH3 cells. Immunoblots indicate that the steroid also increases the expression of the 76 kd Kv1.5 protein 3-fold within 12 hr without altering its half-life (t 1 2 ≈ 4 hr). In contrast, Kv1.4 protein expression is unaffected. Finally, we find that the induction of Kv1.5 protein is associated with an increase in a noninactivating component of the voltage-gated K+ current. Our results indicate that hormones and neurotransmitters may act within hours to regulate excitability by controlling K+ channel gene expression.
AB - Glucocorticoids specifically increase Kv1.5 K+ channel mRNA in normal and clonal (GH3) rat pituitary cells. Here, we demonstrate that dexamethasone, a glucocorticoid agonist, rapidly induces Kv1.5 gene transcription, but does not affect Kv1.5 mRNA turnover t 1 2 ≈ 0.5 hr) in GH3 cells. Immunoblots indicate that the steroid also increases the expression of the 76 kd Kv1.5 protein 3-fold within 12 hr without altering its half-life (t 1 2 ≈ 4 hr). In contrast, Kv1.4 protein expression is unaffected. Finally, we find that the induction of Kv1.5 protein is associated with an increase in a noninactivating component of the voltage-gated K+ current. Our results indicate that hormones and neurotransmitters may act within hours to regulate excitability by controlling K+ channel gene expression.
UR - http://www.scopus.com/inward/record.url?scp=0027328430&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027328430&partnerID=8YFLogxK
U2 - 10.1016/0896-6273(93)90191-S
DO - 10.1016/0896-6273(93)90191-S
M3 - Article
C2 - 8352944
AN - SCOPUS:0027328430
VL - 11
SP - 359
EP - 369
JO - Neuron
JF - Neuron
SN - 0896-6273
IS - 2
ER -