Dexamethasone rapidly increases calcium channel subunit messenger RNA expression and high voltage-activated calcium current in clonal pituitary cells

Alla F Fomina, E. S. Levitan, K. Takimoto

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Glucocorticoid hormones increase voltage-gated Ca2+ current density in clonal pituitary cells. To test whether these steroids might stimulate expression of Ca2+ channel genes, messenger RNase protection assays were used to measure α(1C) and α(1D) RNAs that encode pore-forming subunits of L-type Ca2+ channels. We show here that dexamethasone rapidly increases α(1C) messenger RNA expression without affecting α(1D) messenger RNA level. This up-regulation of channel messenger RNA is also produced by natural glucocorticoids and is blocked by the glucocorticoid antagonist Ru48386. The up-regulation of the channel subunit messenger RNA expression is associated with an increase in high voltage-activated Ca2+ current density. Thus, glucocorticoids may produce a long-term effect on Ca2+ homeostasis in clonal pituitary cells by differentially regulating expression of Ca2+ channel subunit genes.

Original languageEnglish (US)
Pages (from-to)857-862
Number of pages6
JournalNeuroscience
Volume72
Issue number3
DOIs
StatePublished - Jun 1996
Externally publishedYes

Fingerprint

Calcium Channels
Dexamethasone
Glucocorticoids
Calcium
Messenger RNA
Up-Regulation
Ribonucleases
Genes
Homeostasis
Steroids
Hormones
RNA

Keywords

  • calcium channel subunits
  • calcium currents
  • glucocorticoids

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Dexamethasone rapidly increases calcium channel subunit messenger RNA expression and high voltage-activated calcium current in clonal pituitary cells. / Fomina, Alla F; Levitan, E. S.; Takimoto, K.

In: Neuroscience, Vol. 72, No. 3, 06.1996, p. 857-862.

Research output: Contribution to journalArticle

@article{e9e54b83135f4e08a4db8e69656cb3a4,
title = "Dexamethasone rapidly increases calcium channel subunit messenger RNA expression and high voltage-activated calcium current in clonal pituitary cells",
abstract = "Glucocorticoid hormones increase voltage-gated Ca2+ current density in clonal pituitary cells. To test whether these steroids might stimulate expression of Ca2+ channel genes, messenger RNase protection assays were used to measure α(1C) and α(1D) RNAs that encode pore-forming subunits of L-type Ca2+ channels. We show here that dexamethasone rapidly increases α(1C) messenger RNA expression without affecting α(1D) messenger RNA level. This up-regulation of channel messenger RNA is also produced by natural glucocorticoids and is blocked by the glucocorticoid antagonist Ru48386. The up-regulation of the channel subunit messenger RNA expression is associated with an increase in high voltage-activated Ca2+ current density. Thus, glucocorticoids may produce a long-term effect on Ca2+ homeostasis in clonal pituitary cells by differentially regulating expression of Ca2+ channel subunit genes.",
keywords = "calcium channel subunits, calcium currents, glucocorticoids",
author = "Fomina, {Alla F} and Levitan, {E. S.} and K. Takimoto",
year = "1996",
month = "6",
doi = "10.1016/0306-4522(95)00580-3",
language = "English (US)",
volume = "72",
pages = "857--862",
journal = "Neuroscience",
issn = "0306-4522",
publisher = "Elsevier Limited",
number = "3",

}

TY - JOUR

T1 - Dexamethasone rapidly increases calcium channel subunit messenger RNA expression and high voltage-activated calcium current in clonal pituitary cells

AU - Fomina, Alla F

AU - Levitan, E. S.

AU - Takimoto, K.

PY - 1996/6

Y1 - 1996/6

N2 - Glucocorticoid hormones increase voltage-gated Ca2+ current density in clonal pituitary cells. To test whether these steroids might stimulate expression of Ca2+ channel genes, messenger RNase protection assays were used to measure α(1C) and α(1D) RNAs that encode pore-forming subunits of L-type Ca2+ channels. We show here that dexamethasone rapidly increases α(1C) messenger RNA expression without affecting α(1D) messenger RNA level. This up-regulation of channel messenger RNA is also produced by natural glucocorticoids and is blocked by the glucocorticoid antagonist Ru48386. The up-regulation of the channel subunit messenger RNA expression is associated with an increase in high voltage-activated Ca2+ current density. Thus, glucocorticoids may produce a long-term effect on Ca2+ homeostasis in clonal pituitary cells by differentially regulating expression of Ca2+ channel subunit genes.

AB - Glucocorticoid hormones increase voltage-gated Ca2+ current density in clonal pituitary cells. To test whether these steroids might stimulate expression of Ca2+ channel genes, messenger RNase protection assays were used to measure α(1C) and α(1D) RNAs that encode pore-forming subunits of L-type Ca2+ channels. We show here that dexamethasone rapidly increases α(1C) messenger RNA expression without affecting α(1D) messenger RNA level. This up-regulation of channel messenger RNA is also produced by natural glucocorticoids and is blocked by the glucocorticoid antagonist Ru48386. The up-regulation of the channel subunit messenger RNA expression is associated with an increase in high voltage-activated Ca2+ current density. Thus, glucocorticoids may produce a long-term effect on Ca2+ homeostasis in clonal pituitary cells by differentially regulating expression of Ca2+ channel subunit genes.

KW - calcium channel subunits

KW - calcium currents

KW - glucocorticoids

UR - http://www.scopus.com/inward/record.url?scp=0029983265&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029983265&partnerID=8YFLogxK

U2 - 10.1016/0306-4522(95)00580-3

DO - 10.1016/0306-4522(95)00580-3

M3 - Article

C2 - 9157331

AN - SCOPUS:0029983265

VL - 72

SP - 857

EP - 862

JO - Neuroscience

JF - Neuroscience

SN - 0306-4522

IS - 3

ER -