Dexamethasone enhancement of gene expression after direct hepatic DNA injection

Robert W. Malone, M. Anne Hickman, Karin Lehmann-Bruinsma, Tracey R. Sih, Rosemary Walzem, Don M. Carlson, Jerry S Powell

Research output: Contribution to journalArticlepeer-review

73 Scopus citations

Abstract

The critical physiological functions of the liver make hepatocytes important targets for therapeutic gene delivery. This study reports significant gene expression following direct injection of plasmid DNA into the livers of rats and cats. Transfection was characterized using luciferase and Lac Z expression from plasmids with the cytomegalovirus immediate early promoter/enhancer (CMV IE) or the Rous sarcoma virus long terminal repeat (RSV LTR). Dexamethasone treatment enhanced and prolonged transfected gene expression, possibly by activating gene expression. Southern analysis of total DNA extracted from liver at various times following injection detected persistent unintegrated plasmid DNA which maintained a prokaryotic methylation pattern. This study demonstrates the feasibility of direct DNA injection in the experimental analysis of hepatic gene expression in vivo.

Original languageEnglish (US)
Pages (from-to)29903-29907
Number of pages5
JournalJournal of Biological Chemistry
Volume269
Issue number47
StatePublished - Nov 25 1994

ASJC Scopus subject areas

  • Biochemistry

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