Developmental validation of DogFiler, a novel multiplex for canine DNA profiling in forensic casework

Elizabeth Wictum, Teri Kun, Christina Lindquist, Julia Malvick, Dianne Vankan, Benjamin Sacks

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

While the analysis of human DNA has been the focus of large-scale collaborative endeavors, non-human forensic DNA analysis has not benefited from the same funding streams and coordination of effort. Consequently, the development of standard marker panels, allelic ladders and allele-specific sequence data comparable to those established for human forensic genetics has lagged. To meet that need for domestic dogs, we investigated sequence data provided by the published 7.6X dog genome for novel short tandem repeat markers that met our criteria for sensitivity, stability, robustness, polymorphic information content, and ease of scoring. Fifteen unlinked tetranucleotide repeat markers were selected from a pool of 3113 candidate markers and assembled with a sex-linked marker into a multiplex capable of generating a full profile with as little as 60 pg of nuclear DNA. An accompanying allelic ladder was assembled and sequenced to obtain detailed repeat motif data. Validation was carried out according to SWGDAM guidelines, and the DogFiler panel has been integrated into forensic casework and accepted in courts across the U.S. Applying various formulae for calculating random match probabilities for inbred populations, estimates for this panel of markers have proven to be comparable to those obtained in human forensic genetics. The DogFiler panel and the associated allelic ladder represent the first published non-human profiling system to fully address all SWGDAM recommendations.

Original languageEnglish (US)
Pages (from-to)82-91
Number of pages10
JournalForensic Science International: Genetics
Volume7
Issue number1
DOIs
StatePublished - Jan 1 2013

Fingerprint

DNA Fingerprinting
Forensic Genetics
Canidae
Medical Genetics
Microsatellite Repeats
DNA
Dogs
Alleles
Genome
Guidelines
Population

Keywords

  • Canine
  • Dog
  • DogFiler
  • Non-human DNA
  • Validation

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Genetics

Cite this

Developmental validation of DogFiler, a novel multiplex for canine DNA profiling in forensic casework. / Wictum, Elizabeth; Kun, Teri; Lindquist, Christina; Malvick, Julia; Vankan, Dianne; Sacks, Benjamin.

In: Forensic Science International: Genetics, Vol. 7, No. 1, 01.01.2013, p. 82-91.

Research output: Contribution to journalArticle

Wictum, Elizabeth ; Kun, Teri ; Lindquist, Christina ; Malvick, Julia ; Vankan, Dianne ; Sacks, Benjamin. / Developmental validation of DogFiler, a novel multiplex for canine DNA profiling in forensic casework. In: Forensic Science International: Genetics. 2013 ; Vol. 7, No. 1. pp. 82-91.
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