Abstract
While the analysis of human DNA has been the focus of large-scale collaborative endeavors, non-human forensic DNA analysis has not benefited from the same funding streams and coordination of effort. Consequently, the development of standard marker panels, allelic ladders and allele-specific sequence data comparable to those established for human forensic genetics has lagged. To meet that need for domestic dogs, we investigated sequence data provided by the published 7.6X dog genome for novel short tandem repeat markers that met our criteria for sensitivity, stability, robustness, polymorphic information content, and ease of scoring. Fifteen unlinked tetranucleotide repeat markers were selected from a pool of 3113 candidate markers and assembled with a sex-linked marker into a multiplex capable of generating a full profile with as little as 60 pg of nuclear DNA. An accompanying allelic ladder was assembled and sequenced to obtain detailed repeat motif data. Validation was carried out according to SWGDAM guidelines, and the DogFiler panel has been integrated into forensic casework and accepted in courts across the U.S. Applying various formulae for calculating random match probabilities for inbred populations, estimates for this panel of markers have proven to be comparable to those obtained in human forensic genetics. The DogFiler panel and the associated allelic ladder represent the first published non-human profiling system to fully address all SWGDAM recommendations.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 82-91 |
| Number of pages | 10 |
| Journal | Forensic Science International: Genetics |
| Volume | 7 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 2013 |
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Keywords
- Canine
- Dog
- DogFiler
- Non-human DNA
- Validation
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Genetics
Cite this
Developmental validation of DogFiler, a novel multiplex for canine DNA profiling in forensic casework. / Wictum, Elizabeth; Kun, Teri; Lindquist, Christina; Malvick, Julia; Vankan, Dianne; Sacks, Benjamin.
In: Forensic Science International: Genetics, Vol. 7, No. 1, 01.01.2013, p. 82-91.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Developmental validation of DogFiler, a novel multiplex for canine DNA profiling in forensic casework
AU - Wictum, Elizabeth
AU - Kun, Teri
AU - Lindquist, Christina
AU - Malvick, Julia
AU - Vankan, Dianne
AU - Sacks, Benjamin
PY - 2013/1/1
Y1 - 2013/1/1
N2 - While the analysis of human DNA has been the focus of large-scale collaborative endeavors, non-human forensic DNA analysis has not benefited from the same funding streams and coordination of effort. Consequently, the development of standard marker panels, allelic ladders and allele-specific sequence data comparable to those established for human forensic genetics has lagged. To meet that need for domestic dogs, we investigated sequence data provided by the published 7.6X dog genome for novel short tandem repeat markers that met our criteria for sensitivity, stability, robustness, polymorphic information content, and ease of scoring. Fifteen unlinked tetranucleotide repeat markers were selected from a pool of 3113 candidate markers and assembled with a sex-linked marker into a multiplex capable of generating a full profile with as little as 60 pg of nuclear DNA. An accompanying allelic ladder was assembled and sequenced to obtain detailed repeat motif data. Validation was carried out according to SWGDAM guidelines, and the DogFiler panel has been integrated into forensic casework and accepted in courts across the U.S. Applying various formulae for calculating random match probabilities for inbred populations, estimates for this panel of markers have proven to be comparable to those obtained in human forensic genetics. The DogFiler panel and the associated allelic ladder represent the first published non-human profiling system to fully address all SWGDAM recommendations.
AB - While the analysis of human DNA has been the focus of large-scale collaborative endeavors, non-human forensic DNA analysis has not benefited from the same funding streams and coordination of effort. Consequently, the development of standard marker panels, allelic ladders and allele-specific sequence data comparable to those established for human forensic genetics has lagged. To meet that need for domestic dogs, we investigated sequence data provided by the published 7.6X dog genome for novel short tandem repeat markers that met our criteria for sensitivity, stability, robustness, polymorphic information content, and ease of scoring. Fifteen unlinked tetranucleotide repeat markers were selected from a pool of 3113 candidate markers and assembled with a sex-linked marker into a multiplex capable of generating a full profile with as little as 60 pg of nuclear DNA. An accompanying allelic ladder was assembled and sequenced to obtain detailed repeat motif data. Validation was carried out according to SWGDAM guidelines, and the DogFiler panel has been integrated into forensic casework and accepted in courts across the U.S. Applying various formulae for calculating random match probabilities for inbred populations, estimates for this panel of markers have proven to be comparable to those obtained in human forensic genetics. The DogFiler panel and the associated allelic ladder represent the first published non-human profiling system to fully address all SWGDAM recommendations.
KW - Canine
KW - Dog
KW - DogFiler
KW - Non-human DNA
KW - Validation
UR - http://www.scopus.com/inward/record.url?scp=84869493178&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84869493178&partnerID=8YFLogxK
U2 - 10.1016/j.fsigen.2012.07.001
DO - 10.1016/j.fsigen.2012.07.001
M3 - Article
C2 - 22832398
AN - SCOPUS:84869493178
VL - 7
SP - 82
EP - 91
JO - Forensic Science International: Genetics
JF - Forensic Science International: Genetics
SN - 1872-4973
IS - 1
ER -