Developmental expression of P0 mRNA and P0 protein in the sciatic nerve and the spinal nerve roots of the rat

Pierluigi Baron, Michael Shy, Hitoshi Honda, Maria Sessa, John Kamholz, David E Pleasure

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Expression of myelin P0 protein by myelinating Schwann cells in vivo is dependent on axonal influences. This report describes P0 gene expression during development of rat sciatic nerve and spinal nerve roots using Northern blotting, in situ hybridization and immunohistochemistry. We demonstrate that: (1) the appearance of P0 mRNA and P0 protein in Schwann cells during nerve development in the rat begins prenatally, at day 18 post-fertilization (E18); (2) P0 mRNA and P0 protein have essentially identical developmental profiles, and are expressed in Schwann cells that are many days prior to myelin formation; (3) initial P0 gene expression is greatest in Schwann cells at the periphery of nerve bundles and in Schwann cells in contact with motor axons; (4) the decline in P0 expression with nerve maturation is accompanied by a sharp decline in P0 message levels in most Schwann cells, but a small subpopulation of these cells continue to synthesize very high levels of P0 mRNA. This study provides data on myelin P0 protein gene expression and distribution during PNS development and adds further insights into the axonal influences controlling Schwann cell behaviour during myelination of the rat PNS.

Original languageEnglish (US)
Pages (from-to)249-257
Number of pages9
JournalJournal of Neurocytology
Volume23
Issue number4
DOIs
StatePublished - Apr 1994
Externally publishedYes

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Myelin P0 Protein
Spinal Nerve Roots
Schwann Cells
Sciatic Nerve
Messenger RNA
Gene Expression
Myelin Sheath
Fertilization
Northern Blotting
In Situ Hybridization
Axons
Immunohistochemistry

ASJC Scopus subject areas

  • Neuroscience(all)
  • Histology
  • Anatomy
  • Cell Biology

Cite this

Developmental expression of P0 mRNA and P0 protein in the sciatic nerve and the spinal nerve roots of the rat. / Baron, Pierluigi; Shy, Michael; Honda, Hitoshi; Sessa, Maria; Kamholz, John; Pleasure, David E.

In: Journal of Neurocytology, Vol. 23, No. 4, 04.1994, p. 249-257.

Research output: Contribution to journalArticle

Baron, Pierluigi ; Shy, Michael ; Honda, Hitoshi ; Sessa, Maria ; Kamholz, John ; Pleasure, David E. / Developmental expression of P0 mRNA and P0 protein in the sciatic nerve and the spinal nerve roots of the rat. In: Journal of Neurocytology. 1994 ; Vol. 23, No. 4. pp. 249-257.
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