Developmental expression of N-methyl-d-aspartate (NMDA) receptor subunits in human white and gray matter: Potential mechanism of increased vulnerability in the immature brain

Lauren L. Jantzie, Delia M. Talos, Michele C. Jackson, Hyun Kyung Park, Dionne A. Graham, Mirna Lechpammer, Rebecca D. Folkerth, Joseph J. Volpe, Frances E. Jensen

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The pathophysiology of perinatal brain injury is multifactorial and involves hypoxia-ischemia (HI) and inflammation. N-methyl-d-aspartate receptors (NMDAR) are present on neurons and glia in immature rodents, and NMDAR antagonists are protective in HI models. To enhance clinical translation of rodent data, we examined protein expression of 6 NMDAR subunits in postmortem human brains without injury from 20 postconceptional weeks through adulthood and in cases of periventricular leukomalacia (PVL). We hypothesized that the developing brain is intrinsically vulnerable to excitotoxicity via maturation-specific NMDAR levels and subunit composition. In normal white matter, NR1 and NR2B levels were highest in the preterm period compared with adult. In gray matter, NR2A and NR3A expression were highest near term. NR2A was significantly elevated in PVL white matter, with reduced NR1 and NR3A in gray matter compared with uninjured controls. These data suggest increased NMDAR-mediated vulnerability during early brain development due to an overall upregulation of individual receptors subunits, in particular, the presence of highly calcium permeable NR2B-containing and magnesium-insensitive NR3A NMDARs. These data improve understanding of molecular diversity and heterogeneity of NMDAR subunit expression in human brain development and supports an intrinsic prenatal vulnerability to glutamate-mediated injury; validating NMDAR subunit-specific targeted therapies for PVL.

Original languageEnglish (US)
Pages (from-to)482-495
Number of pages14
JournalCerebral Cortex
Volume25
Issue number2
DOIs
StatePublished - 2015
Externally publishedYes

Fingerprint

Periventricular Leukomalacia
Brain
Brain Injuries
Rodentia
Ischemia
Human Development
Gray Matter
aspartic acid receptor
White Matter
Neuroglia
Magnesium
Glutamic Acid
Up-Regulation
Inflammation
Calcium
Neurons
Wounds and Injuries
Proteins
Hypoxia
Therapeutics

Keywords

  • excitotoxicity
  • glutamate
  • hypoxia-ischemia
  • N-methyl-d-aspartate
  • periventricular leukomalacia

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cognitive Neuroscience

Cite this

Developmental expression of N-methyl-d-aspartate (NMDA) receptor subunits in human white and gray matter : Potential mechanism of increased vulnerability in the immature brain. / Jantzie, Lauren L.; Talos, Delia M.; Jackson, Michele C.; Park, Hyun Kyung; Graham, Dionne A.; Lechpammer, Mirna; Folkerth, Rebecca D.; Volpe, Joseph J.; Jensen, Frances E.

In: Cerebral Cortex, Vol. 25, No. 2, 2015, p. 482-495.

Research output: Contribution to journalArticle

Jantzie, Lauren L. ; Talos, Delia M. ; Jackson, Michele C. ; Park, Hyun Kyung ; Graham, Dionne A. ; Lechpammer, Mirna ; Folkerth, Rebecca D. ; Volpe, Joseph J. ; Jensen, Frances E. / Developmental expression of N-methyl-d-aspartate (NMDA) receptor subunits in human white and gray matter : Potential mechanism of increased vulnerability in the immature brain. In: Cerebral Cortex. 2015 ; Vol. 25, No. 2. pp. 482-495.
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