Developmental effects of fipronil on Japanese Medaka (Oryzias latipes) embryos

Scott D. Wagner, Tomofumi Kurobe, Bruce G. Hammock, Chelsea H. Lam, Gary Wu, Natalia Vasylieva, Shirley J. Gee, Bruce D. Hammock, Swee J Teh

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Pesticides in urban runoff are a major source of pollutants in aquatic ecosystems. Fipronil, a phenylpyrazole insecticide, found in structural pest control products, turf grass control, and home pet flea medication, has recently increased in use and is commonly detected in urban runoff. However, little is known about the effects of fipronil on aquatic organisms at early developmental stages. Here, we evaluated toxicity of fipronil to embryos of Japanese Medaka (Oryzias latipes, Qurt strain) using a high-throughput 96-well plate toxicity test. Male and female embryos (<6 h post fertilization) were exposed to concentrations of fipronil ranging from 0.1 to 910 μg L−1 for 14 days or until hatching. Embryos were subjected to gross and microscopic examinations of developmental adverse effects as well as transcriptome analysis using RNA-seq. Results indicated a positive dose-response in reduced hatching success, increased gross deformity (tail curvature) at a lowest-observed-effect concentration (LOEC) of 200 μg L−1 and delayed hatching (∼1 day at the highest concentration, LOEC = 600 μg L−1). The transcriptome analysis indicated that fipronil exposure enhanced expression of titin and telethonin, which are responsible for muscle development. It is therefore possible that the formation of a tail curvature is due to asymmetrical overgrowth of muscle. Our results indicate that sub-lethal effects occur in embryonic stages of an aquatic vertebrate following exposure to high concentrations of fipronil, although no adverse effects at the highest published environmentally relevant concentration (6.3 μg L−1) were observed.

Original languageEnglish (US)
Pages (from-to)511-520
Number of pages10
JournalChemosphere
Volume166
DOIs
StatePublished - Jan 1 2017

Fingerprint

Oryzias
Runoff
Toxicity
Muscle
embryo
Embryonic Structures
Pest control
Aquatic organisms
Aquatic ecosystems
Insecticides
Pesticides
RNA
hatching
Microscopic examination
Throughput
Gene Expression Profiling
curvature
Tail
muscle
Connectin

Keywords

  • Developmental deformity
  • ELISA
  • Multi-model inference
  • RNA-seq

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry

Cite this

Developmental effects of fipronil on Japanese Medaka (Oryzias latipes) embryos. / Wagner, Scott D.; Kurobe, Tomofumi; Hammock, Bruce G.; Lam, Chelsea H.; Wu, Gary; Vasylieva, Natalia; Gee, Shirley J.; Hammock, Bruce D.; Teh, Swee J.

In: Chemosphere, Vol. 166, 01.01.2017, p. 511-520.

Research output: Contribution to journalArticle

Wagner, SD, Kurobe, T, Hammock, BG, Lam, CH, Wu, G, Vasylieva, N, Gee, SJ, Hammock, BD & Teh, SJ 2017, 'Developmental effects of fipronil on Japanese Medaka (Oryzias latipes) embryos', Chemosphere, vol. 166, pp. 511-520. https://doi.org/10.1016/j.chemosphere.2016.09.069
Wagner, Scott D. ; Kurobe, Tomofumi ; Hammock, Bruce G. ; Lam, Chelsea H. ; Wu, Gary ; Vasylieva, Natalia ; Gee, Shirley J. ; Hammock, Bruce D. ; Teh, Swee J. / Developmental effects of fipronil on Japanese Medaka (Oryzias latipes) embryos. In: Chemosphere. 2017 ; Vol. 166. pp. 511-520.
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AU - Wu, Gary

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